Centromere Protein

Centromere Protein

Centromere Protein

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  • View Data Sheet

    Description:

    Centromere Protein-U Human Recombinant

    Centromere Protein U, MLF1IP, Polo-Box-Interacting Protein 1, MLF1 Interacting Protein, Centromere Protein Of 50 KDa, Interphase Centromere Complex Protein 24, KLIP1, PBIP1, KSHV Latent Nuclear Antigen-Interacting Protein 1, MLF1-Interacting Protein, CENP50, CENPU50, KSHV Latent Nuclear Antigen Interacting Protein 1, CENP-50, CENP-U, ICEN24.

    Product # :

    PRO-1824

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    Description

    CENPU Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 295 amino acids (147-418a.a) and having a molecular mass of 33.7kDa.CENPU is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CENPU protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.

    Purity

    Greater than 80.0% as determined by SDS-PAGE.

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    • Introduction

      Centromere Protein U, also known as CENPU is a specialized chromatin domain, present throughout the cell cycle. CENPU performs as a platform on which the transient assembly of the kinetochore takes place during mitosis. CENPU plays a major role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein is responsible for the first recruitment and maintenance of the kinetochore PLK1 population until its degradation. CENPU is also implicated in transcriptional repression.

    • Synonyms

      Centromere Protein U, MLF1IP, Polo-Box-Interacting Protein 1, MLF1 Interacting Protein, Centromere Protein Of 50 KDa, Interphase Centromere Complex Protein 24, KLIP1, PBIP1, KSHV Latent Nuclear Antigen-Interacting Protein 1, MLF1-Interacting Protein, CENP50, CENPU50, KSHV Latent Nuclear Antigen Interacting Protein 1, CENP-50, CENP-U, ICEN24.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenpu Human
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    Description:

    Centromere Protein-Q Human Recombinant

    Centromere protein Q, CENPQ, CENP-Q, C6orf139, Centromere Protein-Q.

    Product # :

    PRO-1815

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    Description

    CENPQ Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 291 amino acids (1-268a.a) and having a molecular mass of 33kDa.CENPQ is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CENPQ protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      CENPQ is a Component of a CENPH-CENPI-associated centromeric complex which targets CENPA to centromeres and is necessary for proper kinetochore function and mitotic progression. CENPQ takes part in incorporation of newly synthesized CENPA into centromeres using its interaction with the CENPA-NAC complex.

    • Synonyms

      Centromere protein Q, CENPQ, CENP-Q, C6orf139, Centromere Protein-Q.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSGKANA SKKNAQQLKR NPKRKKDNEE VVLSENKVRN TVKKNKNHLK DLSSEGQTKH TNLKHGKTAA SKRKTWQPLS KSTRDHLQTM MESVIMTILS NSIKEKEEIQ YHLNFLKKRL LQQCETLKVP PKKMEDLTNV SSLLNMERAR DKANEEGLAL LQEEIDKMVE TTELMTGNIQ SLKNKIQILA SEVEEEEERV KQMHQINSSG VLSLPELSQK TLKAPTLQKE ILALIPNQNA LLKDLDILHN SSQMKSMSTF IEEAYKKLDA S.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenpq Human
  • View Data Sheet

    Description:

    Centromere Protein-P Human Recombinant

    Centromere protein P, CENP-P, CENPP.

    Product # :

    PRO-1784

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    Description

    CENPP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-288 a.a) and having a molecular mass of 35.3kDa.CENPP is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CENPP protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      Centromere protein P (CENPP) is component of the CENPA-CAD (nucleosome distal) complex, which is a complex recruited to centromeres and involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPP protein is involved in incorporation of newly synthesized CENPA into centromeres by way of its interaction with the CENPA-NAC complex.

    • Synonyms

      Centromere protein P, CENP-P, CENPP.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MDAELAEVRA LQAEIAALRR ACEDPPAPWE EKSRVQKSFQ AIHQFNLEGW KSSKDLKNQL GHLESELSFL STLTGINIRN HSKQTEDLTS TEMTEKSIRK VLQRHRLSGN CHMVTFQLEF QILEIQNKER LSSAVTDLNI IMEPTECSEL SEFVSRAEER KDLFMFFRSL HFFVEWFEYR KRTFKHLKEK YPDAVYLSEG PSSCSMGIRS ASRPGFELVI VWRIQIDEDG KVFPKLDLLT KVPQRALELD KNRAIETAPL SFRTLVGLLG IEAALESLIK SLCAEENN.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenpp Human
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    Description:

    Centromere Protein-M Human Recombinant

    Centromere Protein M, Interphase Centromere Complex Protein 39, Chromosome 22 Open Reading Frame 18, Proliferation Associated Nuclear Element 1, bK250D10.2, CENP-M, ICEN39, C22orf18, PANE1.

    Product # :

    PRO-1626

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    Description

    CENPM Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 203 amino acids (1-180) and having a molecular mass of 22.0kDa.CENPM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The CENPM solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.

    • Synonyms

      Centromere Protein M, Interphase Centromere Complex Protein 39, Chromosome 22 Open Reading Frame 18, Proliferation Associated Nuclear Element 1, bK250D10.2, CENP-M, ICEN39, C22orf18, PANE1.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSVLRPL DKLPGLNTAT ILLVGTEDAL LQQLADSMLK EDCASELKVH LAKSLPLPSS VNRPRIDLIV FVVNLHSKYS LQNTEESLRH VDASFFLGKV CFLATGAGRE SHCSIHRHTV VKLAHTYQSP LLYCDLEVEG FRATMAQRLV RVLQICAGHV PGVSALNLLS LLRSSEGPSL EDL

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenpm Human
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    Description:

    Centromere Protein-H Human Recombinant

    Centromere protein H, Interphase centromere complex protein 35, CENP-H, NNF1, PMF1, ICEN35, Kinetochore protein CENP-H.

    Product # :

    PRO-966

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    Description

    CENPH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (136-247) and having a molecular mass of 15.5 kDa.The CENPH is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The CENPH solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      CENPH is a member of the centromere protein H family. CENPH protein is a component of the CENPA-NAC (nucleosome-associated) complex which has a vital part in assembly of kinetochore proteins. The CENPA-NAC complex utilizes the CENPA-CAD (nucleosome distal) complex and is involved in integration of freshly synthesized CENPA into centromeres.

    • Synonyms

      Centromere protein H, Interphase centromere complex protein 35, CENP-H, NNF1, PMF1, ICEN35, Kinetochore protein CENP-H.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenph Human
  • View Data Sheet

    Description:

    Centromere Protein B Human Recombinant

    Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.

    Product # :

    PRO-390

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    Description

    CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9 insect cells.

    Formulation

    CENPB is supplied at a concentration of in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
      CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.

    • Synonyms

      Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.

    • Physical Appearance

      Sterile Filtered liquid formulation.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

    • coating concentration

      0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenpb Human
  • View Data Sheet

    Description:

    Centromere Protein A Human Recombinant

    Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.

    Product # :

    PRO-389

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    Description

    CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

    Source

    Sf9 insect cells.

    Formulation

    CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
      CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients.

    • Synonyms

      Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.

    • Physical Appearance

      Sterile Filtered liquid formulation.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

    • Immunological Functions

      1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

    • coating concentration

      0.15-0.4 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

    • Applications

      Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cenpa Human

About CENP / Centromere Protein:

Centromere proteins are components of the nucleosome distal complex, required by centromeres to assemble kinetochore proteins, chromosome segregation and mitotic progressions. Throughout its history and discovery, centromere protein has had many different names, including Cse4p in yeast, Cid in fruit flies, and Cnplp in fission yeast.

CENP Function
Centromere protein A (CENP-A) is a centromeric variant of the regular histone H3, found mainly in the centromeric region during periods of replication. It plays a vital role in the regulation of centromere formation, genome stability and the constriction of the kinetochore. De novo centromere assembly is dependent on RNA interference and heterochromatic pathways in yeast.

Centromere Protein Structure
This histone variant is different from the H2A histone because it contains a macro domain at the C-terminal end. The protein is only found in vertebrates and is 60 percent similar to its canonical counterpart.
CENP-A is a histone variant, but essential for survival. The fold domain of the protein appears to be evolutionarily conserved, thanks to the high percentage similarity to the H3 histone. In terms of evolution, it appears to be one of the fastest-evolving histones, similar to H3.3.

CENP Mechanism
CENP-A is interesting from a scientific perspective because of its ability to create split nucleosomes in fruit flies. Being able to do this is something that has challenged the chromatin field, but it now appears to be possible.
It has also been shown that recombinant CENP-A and the complementary core histone counterpart may be able to reconstitute full nucleosomes in vitro, representing a significant methodological and experimental breakthrough.