CENPA Human

CENPA Human

Centromere Protein

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  • Name :

    CENPA Human

    Description:

    Centromere Protein A Human Recombinant

    Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.

    Product # :

    PRO-389

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    Quantity :

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  • formulation
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Description

CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques.

Source

Sf9 insect cells.

Formulation

CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.

Purity

Greater than 90% as determined by SDS-PAGE.

More Info

  • Introduction

    Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
    CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients.

  • Synonyms

    Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.

  • Physical Appearance

    Sterile Filtered liquid formulation.

  • Stability

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

  • Immunological Functions

    1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

  • coating concentration

    0.15-0.4 µg/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

  • Applications

    Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Cenpa Human

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