Chromatin Modifying Protein

Chromatin Modifying Protein

Chromatin Modifying Protein

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  • View Data Sheet

    Description:

    Chromatin Modifying Protein 4A Human Recombinant

    Charged multivesicular body protein 4A, C14orf123, HSPC134, VPS32A, Vacuolar protein sorting-associated protein 32-1, chromatin modifying protein 4A, SHAX2, SNF7-1, SNF7 homolog associated with Alix-2, chromosome 14 open reading frame 123, CHMP4B, CHMP4a.

    Product # :

    PRO-1024

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    Description

    CHMP4A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-265) and having a molecular mass of 32.0kDa.CHMP4A is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The CHMP4A solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 0.1mM PMSF, 1mM EDTA, 2mM DTT and 50% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

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    • Introduction

      CHMP4A is a member of the SNF7 family and operates as chromatin-modifying protein. CHMP4A is a key component of the endosomal sorting vital for transport complex III (ESCRT-III) which takes part in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. Additionally, during HIV-1 infection, the virus utilizes the ESCRT-III complex to facilitate budding and exocytosis of viral proteins through the connection of CHMP4 and a protein engaged by HIV-1 p6, which exists in viral Gag assembly and budding. CHMP4A is expressed in higher quantities in skeletal muscle, kidney, liver and heart.

    • Synonyms

      Charged multivesicular body protein 4A, C14orf123, HSPC134, VPS32A, Vacuolar protein sorting-associated protein 32-1, chromatin modifying protein 4A, SHAX2, SNF7-1, SNF7 homolog associated with Alix-2, chromosome 14 open reading frame 123, CHMP4B, CHMP4a.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Chmp4A Human
  • View Data Sheet

    Description:

    Chromatin Modifying Protein 2B Human Recombinant

    Chromatin modifying protein 2B, CHMP2.5, VPS2B, Vacuolar protein sorting-associated protein 2-2, hVps2-2, DMT1, DKFZp564O123, VPS2 homolog B.

    Product # :

    PRO-877

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    Description

    CHMP2B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-213) and having a molecular mass of 26.1 kDa.The CHMP2B is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CHMP2B protein 1mg/ml is supplied in 20mM Tris-HCL, pH-8, 0.1M NaCl, 2mM DTT and 20% Glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      CHMP2B is a member of the vacuolar sorting protein family. CHMP2B is a component of the ESCRT-III complex which is essential for sorting endosomal articles into multivesicular bodies (MVBs), and are also obligatory for the formation of these bodies. CHMP2B is usually found in brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta and leukocytes.

    • Synonyms

      Chromatin modifying protein 2B, CHMP2.5, VPS2B, Vacuolar protein sorting-associated protein 2-2, hVps2-2, DMT1, DKFZp564O123, VPS2 homolog B.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD EEIERQLKAL GVD

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Chmp2B Human
  • View Data Sheet

    Description:

    Chromatin Modifying Protein 2A Human Recombinant

    Charged multivesicular body protein 2a, Chromatin-modifying protein 2a, Vacuolar protein-sorting-associated protein 2-1, Putative breast adenocarcinoma marker BC-2, Vps2-1, hVps2-1, CHMP2A, BC2, CHMP2, BC-2, VPS2, VPS2A.

    Product # :

    PRO-503

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    Description

    CHMP2A Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 242 amino acids (1-222 a.a.) and having a molecular mass of 27.2 kDa.The CHMP2A is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The CHMP2A solution contains 20mM Tris-HCl buffer (pH8.0) , 0.1M NaCl 1mM DTT, and 30% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Chromatin modifying protein 2A (CHMP2A) is a member of the SNF7 family and it is a component of the ESCRT III complex, which is required for MVBs (multivesicular bodies) formation and sorting of endosomal cargo proteins into MVBs.
      The MVB pathway mediates release of transmembrane proteins into the lumen of the lysosome for degradation. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins generally detach from the invaginating membrane before the ILV (intraluminal vesicles) is released. ESCRT-III proteins are thought to mediate the basic vesicle extrusion and/or membrane fission activities, probably in conjunction with the AAA ATPase VPS4.

    • Synonyms

      Charged multivesicular body protein 2a, Chromatin-modifying protein 2a, Vacuolar protein-sorting-associated protein 2-1, Putative breast adenocarcinoma marker BC-2, Vps2-1, hVps2-1, CHMP2A, BC2, CHMP2, BC-2, VPS2, VPS2A.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Chmp2A Human
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    Description:

    Chromatin Modifying Protein 1A Human Recombinant

    Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1, Vps46-1, hVps46-1, CHMP1A, CHMP1, KIAA0047, PCOLN3, PRSM1, PCH8, VPS46A.

    Product # :

    PRO-1308

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    Description

    CHMP1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (1-196 a.a.) and having a molecular mass of 24.1kDa (Molecular size on SDS-PAGE will appear higher).CHMP1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CHMP1A protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 20% glycerol and 1mM DTT.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Charged multivesicular body protein 1a (CHMP1A) is a member of the vacuolar sorting protein family and functions as chromatin-modifying protein. These complexes are essential for sorting endosomal articles into multivesicular bodies (MVBs), as well as vital for the formation of these bodies. The MVBs pathway mediates distribution of transmembrane proteins into the lumen of the lysosome for degradation. CHMP1 interacts with VPS4B and localizes to early endosomes.

    • Synonyms

      Charged multivesicular body protein 1a, Chromatin-modifying protein 1a, Vacuolar protein sorting-associated protein 46-1, Vps46-1, hVps46-1, CHMP1A, CHMP1, KIAA0047, PCOLN3, PRSM1, PCH8, VPS46A.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMDDTLFQ LKFTAKQLEK LAKKAEKDSK AEQAKVKKAL LQKNVECARV YAENAIRKKN EGVNWLRMAS RVDAVASKVQ TAVTMKGVTK NMAQVTKALD KALSTMDLQK VSSVMDRFEQ QVQNLDVHTS VMEDSMSSAT TLTTPQEQVD SLIMQIAEEN GLEVLDQLSQ LPEGASAVGE SSVRSQEDQL SRRLAALRN.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Chmp1A Human

About CHMP / Chromatin Modifying Protein:

There are many different types of proteins, and Chromatin Modifying Proteins represent a varied and big range of proteins that carry out a number of different epigenetic functions.

CHMP Function
They are able to influence gene expression because they have the ability to modify histones, adjust chromatin conformation, and bind DNA. This means that Chromatin Modifying Proteins are powerful targets for research into epigenome and possible therapeutic targets.

Chromatin Modifying Protein Mechanism
There are a number of different types of Chromatin Modifying Proteins. One example is PcG, which stands for polycomb group proteins. These proteins have the responsibility of differentiating cells throughout their development by the use of transcriptional repression.
There has been an intense amount of study regarding this type of Chromatin Modifying Protein. This is because it is evident that they play a critical role in identifying cell-type, differentiation, and disease by the creation and maintenance of environments that are repressive chromatin by forming one of the two major PRC (polycomb repressive complexes), which are PRC2 and PRC1.
Another type of Chromatin Modifying Protein that we will mention is the HDAC class, which stands for histone deacetylases. These proteins have the responsibility of removing the acetyl groups put on histones by HATs, otherwise known as histone acetyltransferases. This process is a critical element of epigenetic gene expression regulation. In more general terms, it is also imperative in terms of cellular stability control.
Other types of Chromatin Modifying Protein s include zinc finger proteins, heterochromatin protein 1, histone acetyltransferases, and much more.