TRPT1 Human

TRPT1 Human

Enzymes

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Description

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  • Name :

    TRPT1 Human

    Description:

    tRNA Phosphotransferase 1 Human Recombinant

    tRNA 2'-phosphotransferase 1, TRPT1.

    Product # :

    ENZ-208

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  • More Info

Description

TRPT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 277 amino acids (1-253) and having a molecular mass of 30.3kDa.TRPT1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

The TRPT1 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

Purity

Greater than 95.0% as determined by SDS-PAGE.

More Info

  • Introduction

    tRNA 2'-phosphotransferase 1 (TRPT1) is a member of the KptA/TPT1 family. TRPT1 catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. TRPT1 is broadly expressed; it is weakly or not expressed in the lung, spleen, small intestine and peripheral blood leukocytes.

  • Synonyms

    tRNA 2'-phosphotransferase 1, TRPT1.

  • Physical Appearance

    Sterile Filtered colorless solution.

  • Stability

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

  • Amino Acid Sequence

    MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Trpt1 Human

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