Taq Plus DNA

Taq Plus DNA

DNA Polymerase

Name

Description

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  • Name :

    Taq Plus DNA

    Description:

    Taq Plus DNA Polymerase Recombinant

    Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA.

    Product # :

    ENZ-309

    Price :

    Quantity :

    Shipping Method :

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  • description
  • source
  • formulation
  • More Info

Description

Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C.

Source

Recombinant E.coli contains Thermus aquaticus polymerase gene.

Formulation

5U/µl.

More Info

  • Synonyms

    Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA.

  • Stability

    Stable for 5 days at 10°C, for longer period of time store at -20°C.

  • Optimization of DNA Synthesis

    It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus.

  • Reaction Conditions

    DNA synthesis is performed in 100?l of mixture containing 20-200μm dNTPs, 0.3-1μm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA.

  • Storage Buffer

    20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.

  • Note

    All reagents, including Taq Plus, should be mixed immediately before use.

  • Unit Definition

    One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Taq Plus Dna

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