T7 RNAP

T7 RNAP

Enzymes

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Description

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  • Name :

    T7 RNAP

    Description:

    T7 RNA Polymerase Recombinant

    T7 RNAP.

    Product # :

    ENZ-1180

    Price :

    Quantity :

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Description

T7 RNA polymerase Recombinant protein is produced by bacteriophage T7 DNA which is expressed in recombinant E. coli bacterial system T7 RNA Polymerase is a DNA-dependent 5'→ 3' RNA polymerase which specifically recognizes T7 promoter sequences.

Source

T7 Bacteriophage RNA Polymerase gene

Formulation

Transcription Buffer 40mM Tris-HCl (25°C, pH-8), 20mM MgCl2, 2.5mM TCEP & 2mM spermidine.

Purity

Greater than 95% as visualized by SDS-PAGE

More Info

  • Introduction

    T7 RNA polymerase active enzyme synthesizes RNA at an increasing degree of that of E. coli RNA polymerase and it terminates transcription often. T7 RNA polymerase is very selective for initiation at its own promoter sequences and is resistant to antibiotics that inhibit E. coli RNA polymerase. In-vitro transcription of mRNA is achieved via bacteriophage T7 RNA polymerase using its ability to produce full-length RNA transcripts with high reliability, thoughT7 RNAP can manufacture as well immunostimulatory by products for example dsRNA which affect protein expression.

  • Synonyms

    T7 RNAP.

  • Physical Appearance

    Sterile Filtered clear solution.

  • Stability

    Two years when stored at -20°C, 2 weeks at 4°C. DO NOT STORE AT -70C.

  • Applications

    Synthesis of :

    • ssRNAs.
    • Labeled or unlabeled highly specific RNA probes.
    • Capped mRNA using cap analogues.
  • Unit Definition

    1U is defined as the amount of enzyme required to incorporate 1nmol of [3H] ATP into acid-insoluble precipitates within 1 hour at 37℃, pH-8.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

T7 Rnap

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