T4 DNA

T4 DNA

Enzymes

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  • Name :

    T4 DNA

    Description:

    T4 DNA Ligase Recombinant

    DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.

    Product # :

    ENZ-286

    Price :

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  • More Info

Description

T4 DNA Ligase 55.3kDa protein catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Source

E.Coli, cloned gene-30, bacteriophage T4.

Formulation

50% glycerol, 20mM Tris-HCl (pH-7.5), 50mM KCl, 1mM DTT and 0.1mM EDTA.

More Info

  • Synonyms

    DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.

  • Physical Appearance

    400U/ul solution.

  • Stability

    Store T4 DNA Ligase at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

  • Applications

    Cloning of restriction enzyme generated DNA fragments.

    Cloning of PCR products.

    Joining of double-stranded oligonucleotide linkers or adaptors to DNA.

    Site-directed mutagenesis.

    Amplified fragment length polymorphism (AFLP).

    Ligase-mediated RNA detection.

    Nick repair in duplex DNA, RNA or DNA/RNA hybrids.

    Self-circularization of linear DNA

  • Reaction Conditions

    50mM Tris-HCl, pH7.5 at 25°C, 10mM MgCl2, 10mM DTT and 1mM ATP. Incubate at 16 °C

  • Inactivation

    T4 DNA Ligase can be inhibited by NaCl/KCl at a concentrations >than 200mM or by heating at 65 °C for 10 min or at 70 °C for 5 min.

  • Unit Definition

    1U is the amount of T4 DNA Ligase required to ligate > than 50% DNA fragments in a 20μl ligation reaction system, 6μg of λDNA-Hind III decomposition product reacts at 16°C for 30 minutes.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

T4 Dna Ligase

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