SORD Human, His

SORD Human, His

Dehydrogenase

Name

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  • Name :

    SORD Human, His

    Description:

    Sorbitol Dehydrogenase Human Recombinant, His Tag

    EC 1.1.1.14, SORD1,SORD, L-iditol 2-dehydrogenase, DHSO, Sorbitol Dehydrogenase.

    Product # :

    ENZ-520

    Price :

    Quantity :

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

SORD Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 377 amino acids (1-357 a.a.) and having a molecular mass of 40.4 kDa. SORD protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

Source

Escherichia Coli.

Formulation

SORD protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl pH-8, 0.2M NaCl, 5mM DTT & 20% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Biological Activity

Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 25°C.

More Info

  • Introduction

    SORD enzyme is part of of the zinc-containing alcohol dehydrogenase family that is broadly expressed in kidney and in the lens eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses.

  • Synonyms

    EC 1.1.1.14, SORD1,SORD, L-iditol 2-dehydrogenase, DHSO, Sorbitol Dehydrogenase.

  • Physical Appearance

    Sterile filtered colorless solution.

  • Stability

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

  • Amino Acid Sequence

    MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Sord Human

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