SODA E.Coli

SODA E.Coli

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  • Name :

    SODA E.Coli

    Description:

    Superoxide Dismutase E.Coli Recombinant

    Superoxide dismutase [Mn], MnSOD, soda, b3908, JW3879.

    Product # :

    PRO-208

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

SODA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 226 amino acids (1-206 a.a.) and having a molecular mass of 25.2kDa.SODA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

SODA E.coli solution (1mg/ml) containining 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.

Purity

Greater than 95% as determined by SDS-PAGE.

Biological Activity

Specific activity is > 350 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume.

More Info

  • Introduction

    Superoxide dismutase Mn (soda) belongs to the iron/manganese superoxide dismutase family. SodA destroys radicals that are typically produced within the cells and which are toxic to biological systems. SodA works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase.

  • Synonyms

    Superoxide dismutase [Mn], MnSOD, soda, b3908, JW3879.

  • Physical Appearance

    Sterile filtered colorless solution.

  • Stability

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

  • Amino Acid Sequence

    MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS
    GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Soda Ecoli

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