CCL23 Human

CCL23 Human

Chemokines

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Description

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  • Name :

    CCL23 Human

    Description:

    Macrophage Inflammatory Protein-3 Human Recombinant (CCL23)

    C-C motif chemokine 23, Small-inducible cytokine A23, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, CK-beta-8, MIP-3, MPIF-1, CKB-8, CCL23, MIP3, MPIF1, SCYA23, CKb8, Ckb-8-1.

    Product # :

    CHM-358

    Price :

    Quantity :

    Shipping Method :

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    Shipped at Room temp

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

MIP-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 99 amino acids and having a molecular mass of 11.3kDa. The MIP-3 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

Filtered (0.2µm) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

Determined by its ability to chemoattract human T cell population using a concentration range of 10-50ng/ml corresponding to a Specific Activity of 20,000-100,000IU/mg.

More Info

  • Introduction

    CCL23 (MIP-3) is a ligand for the CCR1chemokine receptor. CCL23 is one of several cytokine genes clustered on the q-arm of chromosome 17, in a locus containing several other CC chemokines. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but not activated lymphocytes. Furthermore, it was shown that MIP-3 inhibits colony formation of bone marrow myeloid immature progenitors. MIP-3 is mainly expressed in lung and liver tissue, but can be also found in bone marrow and placenta, as well as in some cell lines of myeloid origin.
    Alternative splicing of the CCL23 gene produces 2 mRNAs which encode a short (CK?8) and a long (CK?81) isoform of the MIP-3. CK?8 cDNA encodes a 120 amino acid residue precursor protein with a putative 21 a.a. residue signal peptide which is cleaved to generate a 99 a.a. residue mature CK?8 (a.a. 22-120). Further N-terminal processing of the 99 a.a. residue variant can produce a 75 a.a. residue CK?8 (a.a. 46-120) which is considerably more active than the 99 a.a. residue variant.
    MIP-3 may be involved in the malignant progression of certain human cancer cells which overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. MIP-3 may also be involved in angiogenesis via upregulation of matrix metalloproteinase MMP-2 expression.

  • Synonyms

    C-C motif chemokine 23, Small-inducible cytokine A23, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, CK-beta-8, MIP-3, MPIF-1, CKB-8, CCL23, MIP3, MPIF1, SCYA23, CKb8, Ckb-8-1.

  • Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

  • Stability

    Lyophilized MIP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL23 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized MIP-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

  • Amino Acid Sequence

    RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.

  • Background

    What is the molecular weight/Mw of CCL23 HUMAN Protein?

    CCL23 HUMAN Protein has a total Mw of 11.3kDa.

     

    What is the source or expression system of CCL23 HUMAN
    Protein?

    Escherichia Coli.

     

    What is the Purity of CCL23 HUMAN Protein?

    CCL23 HUMAN Protein is > 97% pure as determined by SDS-PAGE.

     

    What is the Biological Activity of CCL23 HUMAN Protein?

    Determined by its ability to chemoattract human T cell
    population using a concentration range of 10-50ng/ml corresponding to a
    Specific Activity of 20,000-100,000IU/mg.

     

    What is the amino acid sequence of CCL23 HUMAN Protein?

    RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR
    SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.

     

    What applications can CCL23 HUMAN Protein be used in?

    CCL23 HUMAN Protein can probably be used in western blot,
    ELISA and Lateral Flow.

     

    What is the endotoxin level for CCL23 HUMAN Protein?

    The endotoxin level is minimal, CCL23 HUMAN Protein was
    purified using conventional chromatography techniques.

     

    Who supplies recombinant CCL23 HUMAN that is in stock and
    available to order?

    ProSpec has the protein in stock and available for immediate
    delivery.

     

    Where can I order bulk mg quantities of CCL23 HUMAN?

    ProSpec supplies bulk mg quantities at affordable prices
    that meets research and academic budgets.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Mip 3 Human

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Frequently asked questions

1. Which company offers Recombinant Chemokines at competitive prices?

ProSpec offers vast array of Chemokines such as Eotaxin | Fractalkine | IL-8 | Rantes and much more at affordable descent prices to meet academic budgets
ProSpec provides High Purity Chemokines for 30 years, purity exhibits 95% and more as visualized by SDS-PAGE gel.
Chemokines are in stock for immediate delivery via FedEx which enables scientists and researchers to perform immediate experiments.
Chemokines are shipped globally using ProSpec’s custom tailored Styrofoam insulated box and premium ice packs that ensure maximum protein stability during transit to your laboratory.

2. Who can produce a custom Chemokine ?

ProSpec custom produces recombinant chemokines starting from Gene Design, Gene cloning, Expression Screening, Fermentation + protein expression, Cell harvesting + disruption, Protein recovery and purification to Q.C. formulation and filling, lyophilization is offered as an option.

3. What is the production time for a custom Chemokine?

The production time for a custom recombinant chemokine protein may vary between 5-7 weeks.

4. Where can i find biologically active Chemokines?

ProSpec offers biologically functional Chemokines such as SDF1A | Platelet Factor-4 | MCP1 which are rigorously tested in-vitro to provide researchers world wide BioActive Chemokines.

5. What is the difference between CC, CXC, CX3C, and XC chemokines?

Chemokines are a superfamily of small, secreted signaling proteins that direct the migration of immune cells, they are classified into 4 subfamilies based on the spacing of the cysteine residues near their N-terminus. CC and CXC are the largest families, while CX3C and XC contain fewer members. You can find CXC chemokines and CC chemokines at ProSpecBio such as cat# chm-348 Recombinant Human BCA-1/ BLC (CXCL13), cat# chm-001, Recombinant Human BRAK (CXCL14), cat#
chm-029, Recombinant Human CXCL16, cat# chm-331 Recombinant Human ENA78 (CXCL5) and more....

6. Which receptors bind to a specific chemokine?

Chemokines mainly bind G-protein-coupled receptors ( GPCR ), located on the surfaces of target cells. They match the structural classification of their ligands. Chemokines bind more than one receptor. For instance, CCL2 primarily binds CCR2, while CXCL12 binds CXCR4 and ACKR3. You can find CCL2 chemokine at ProSpecBio cat# chm-271, MCP1.