IL6 Canine

IL6 Canine

Cytokines

Name

Description

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  • Name :

    IL6 Canine

    Description:

    Canine IL-6 Recombinant

    IL6, IL-6, Interleukin-6.

    Product # :

    CYT-1006

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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  • description
  • source
  • formulation
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  • More Info
  • SDS-PAGE

Description

IL6 Canine Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 195 amino acids (21-207a.a.) and having a molecular mass of 22.0kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).IL6 is expressed with an 8 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Formulation

IL6 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

SDS-PAGE

More Info

  • Introduction

    Il-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.

  • Synonyms

    IL6, IL-6, Interleukin-6.

  • Physical Appearance

    Sterile Filtered colorless solution.

  • Stability

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

  • Amino Acid Sequence

    FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEC VKHTTIHLIL RSLEDFLQFS LRAVRIMLEH HHHHH.

  • Background

    Canine IL-6 Recombinant: Implications for Immunotherapy and Veterinary Medicine

    Abstract:

    Interleukin-6 (IL-6) plays a pivotal role in the immune response and inflammation regulation in various species, including canines. The advent of recombinant DNA technology has enabled the production of Canine IL-6 Recombinant (cIL-6r), opening new avenues for research in immunotherapy and veterinary medicine. This paper delves into the significance of cIL-6r, its production methods, and its potential applications in the treatment of inflammatory and autoimmune diseases in dogs.

    Introduction:

    Interleukin-6 is a multifunctional cytokine that exerts its effects on a wide range of physiological processes, including immune responses, hematopoiesis, and inflammation. In the canine immune system, IL-6 plays a crucial role in coordinating immune cell activation, antibody production, and acute phase responses. Recombinant IL-6 production has emerged as a promising strategy to harness its therapeutic potential.

    Methods:

    The production of cIL-6r involves recombinant DNA technology, where the canine IL-6 gene is inserted into an expression vector and transfected into a suitable host cell line, typically bacterial or mammalian cells. The recombinant protein is then purified using various chromatographic techniques to ensure high purity and biological activity.

    Applications:

    Canine IL-6 Recombinant holds immense promise in various applications within veterinary medicine. Its immunomodulatory properties make it a potential candidate for treating conditions such as immune-mediated diseases, inflammatory disorders, and certain types of cancer in dogs. Additionally, cIL-6r can be utilized to stimulate immune responses in vaccines, thereby enhancing their efficacy.

    Challenges and Future Directions:

    While cIL-6r shows great potential, its therapeutic use requires comprehensive studies to establish optimal dosages, safety profiles, and potential side effects. Long-term effects and potential interactions with existing treatments must also be explored.

    Conclusion:

    The advent of Canine IL-6 Recombinant marks a significant advancement in veterinary medicine, offering new avenues for immunotherapy and disease management in dogs. With further research and development, cIL-6r could become an invaluable tool in treating various conditions, ultimately enhancing the health and well-being of our canine companions.

    What is the molecular weight/Mw of IL6 CANINE Protein?
    IL6 CANINE Protein has a total Mw of 22kDa.

    What is the source or expression system of IL6 CANINE Protein?
    Sf9, Baculovirus cells.
    What is the Purity of IL6 CANINE Protein?
    IL6 CANINE Protein is >95% pure as determined by SDS-PAGE.

    What is the Biological Activity of IL6 CANINE Protein?
    The biological functionality of IL6 CANINE Protein will be determined in the future.

    What is the amino acid sequence of IL6 CANINE Protein?
    FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEC VKHTTIHLIL RSLEDFLQFS LRAVRIMLEH HHHHH.

    What applications can IL6 CANINE Protein be used in?
    IL6 CANINE Protein can probably be used in western blot, ELISA and Lateral Flow.

    What is the endotoxin level for IL6 CANINE Protein?
    The endotoxin level is minimal, IL6 CANINE Protein was purified using conventional chromatography techniques.

    Who supplies recombinant IL6 CANINE that is in stock and available to order?
    ProSpec has the protein in stock and available for immediate delivery.

    Where can I order bulk mg quantities of IL6 CANINE?
    ProSpec supplies bulk mg quantities at affordable prices that meets research and academic budgets.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Il6 Canine

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Frequently asked questions

1. Which company offers Recombinant cytokines at competitive prices?

ProSpec offers vast array of cytokines such as Interferon-alpha | Interleukin-2 | TNF-alpha | BMP-2 and much more at affordable descent prices to meet academic budgets.
ProSpec provides High Purity cytokines for 30 years, purity exhibits 95% or more by SDS-PAGE gel.
Cytokines are in stock for immediate delivery via FedEx which enables scientists and researchers to perform immediate experiments.
Cytokines are shipped globally using ProSpec’s custom tailored Styrofoam insulated box and premium ice packs that ensure maximum protein stability during transit to your laboratory.

2. I would like to produce a custom cytokine, who can produce it for me?

ProSpec custom produces recombinant cytokines starting from Gene Design, Gene cloning, Expression Screening, Fermentation + protein expression, Cell harvesting + disruption, Protein recovery and purification to Q.C. formulation and filling, lyophilization is offered as an option.

3. What is the production time for a custom cytokine?

The production time line for a custom recombinant cytokine protein may vary between 5-7 weeks.

4. Where can i find biologically active cytokines?

ProSpec offers biologically functional cytokines such as Adiponectin | Cardiotrophin | Beta Defensin which are rigorously tested in-vitro to provide researchers world wide BioActive Cytokines.

5. What is the activity validation method for Cytokines?

ProSpec uses cell-based bioassays that measures the biological activity, for example:
Adiponectin is validated using AMPK phosphorylation assay, glucose uptake assay.
Angiopoietin is validated using Endothelial tube formation assay, Tie2 phosphorylation assay.
Apolipoprotein is validated using Lipid-binding assay, receptor-binding assay.
Beta Defensin is validated using Antimicrobial assay.
Betacellulin is validated using Cell proliferation assay (EGFR-positive cells).
Bone Morphogenetic Protein is validated using Alkaline phosphatase induction assay.

6. Is the cytokine full length or partial?

The cytokine proteins are both full length and partial while majority of them are active mature proteins rather than a full-length precursor. ProSpec active mature proteins exhibits higher expression yields, better stability, Easier purification and Equivalent biological activity as full length precursor.

7. Is the cytokine monomeric or dimeric form?

This depends on the cytokine. ProSpec cytokines are supplied in the native biologically active oligomeric state, which can be monomeric, dimeric, trimeric, or even multimeric since because receptor binding and biological activity often depend on correct oligomerization.
The interleukin and interferon protein family are usually provided as monomer proteins though adiponectin and TNFSF8 are supplied as homodimers.

8. What is the cytokine purity and how was it determined?

The cytokine purity ranges from high purity of ≥95% to premium grade ≥97%.
ProSpec uses SDS-PAGE Coomassie Blue Staining as the primary purity method which Visualizes protein bands. Some SDS-PAGE gels are stained with Silver Stain. Other analytical methods include RP-HPLC [ High-resolution purity determination ] , SEC-HPLC for the detections of aggregates and fragments and Western Blot to confirm cytokine identity.

9. Is the cytokine sequence verified?

The amino acid sequence verification is performed at DNA & protein level.
Sanger DNA Sequencing verifies the expression plasmid having correct coding.
LC-MS is used to verify the molecular weight of the final purified cytokine
Prospec also uses Edman N-terminal Sequencing to determine the amino-terminal sequence.

10. What is the endotoxin level in cytokines and how do you test LPS ?

ProSpec cytokines are tested for endotoxin also named LPS [ lipopolysaccharide ] which is found in the outer membrane of Gram-negative bacteria which can affect cytokine secretion, cell proliferation and stem cell.
ProSpec measures endotoxin using the Limulus Amebocyte Lysate [ LAL ] assay such as Gel-Clot.
ProSpec standard Endotoxin level for cytokines is usually <1 EU/ug.
What Does "EU" Mean? EU = Endotoxin Units

11. Is there host cell protein contamination?

There might be a minor existence of host-cell proteins post chromatography purification and quality-control procedures that greatly minimize these impurities. ProSpec tests host-cell protein levels by SDS-PAGE silver staining, or mass spectrometry.

12. Is the protein tested in vitro, in-vivo cell culture, stem cells, T cells, ELISA, western blot?

PoSpec Growth Factors proteins are usually tested for biological activity and identity, while some proteins are not though supported by literature and customer experience. Interleukins are tested in T-cell, NK-cell, PBMC, stem cell culture. Interferons are tested in Antiviral assays, immune-cell activation, TNFA proteins are tested in Apoptosis, inflammation and immune studies. ProSpec does not conduct in-vivo or animal injections experiments in house or our source.