HIV-1 TAT Clade-D

HIV-1 TAT Clade-D

HIV

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  • Name :

    HIV-1 TAT Clade-D

    Description:

    HIV-1 TAT Clade-D Recombinant

    Product # :

    HIV-157

    Price :

    Quantity :

    Shipping Method :

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    Shipped at Room temp

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Description

HIV-1 TAT Clade-D Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain having the Accession number: AY194029.

Source

Escherichia Coli.

Formulation

Lyophilized with 10% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

More Info

  • Introduction

    Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication (Jones KA, 1994) and infectivity (Arya SK, 1985; Fisher AG, 1986). In addition, during acute infection, Tat is released extracellularly by infected cells (Chang HC, 1997; Ensoli B, 1990) and is taken up by neighboring cells where it transactivates viral replication (Ensoli B, 1993) and increases virus infectivity.
    HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.

  • Physical Appearance

    Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.

  • Stability

    Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 TAT should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Hiv 1 Tat Clade D

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