HaeIII

HaeIII

Enzymes

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Description

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  • Name :

    HaeIII

    Description:

    HaeIII Recombinant

    site-specific DNA-methyltransferase, HaeIVRM.

    Product # :

    ENZ-1203

    Price :

    Quantity :

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  • More Info

Description

HaeIII is a prokaryotic DNA that protects organisms from an unknown DNA. HaeIII’s recognition site is the GGCC nucleotide sequence which means it cleaves DNA at the site 5′-GG/CC-3.

Source

E. coli that carries the HaeIII gene from Haemophilus aegypticus

Purity

Great than 95 % as estimated by SDS-PAGE analyses.

More Info

  • Synonyms

    site-specific DNA-methyltransferase, HaeIVRM.

  • Physical Appearance

    Sterile Filtered colorless solution.

  • Stability

    HaeIII although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles.

  • Background

    HaeIII cleaves the DNA at the positions where the GGCC sequence is found. The cleavage happens between the 2nd and the third nucleotides -G and C. The resulting DNA fragments are known as restriction fragments. HaeIII cuts both strands of DNA in the same location, creating restriction fragments with blunt ends.

  • Storage Buffer

    10mM Tris-HCl (pH 7.4), 1 mM DTT, 50% (v/v) glycerol, 0.1mM EDTA, 50 mM KCl and 200µg/ml BSA.

  • Unit Definition

    1 unit is defined as the amount of HaeIII required to digest 1 µg of lambda DNA in 1 hour at 37°C in 50 µL of recommended reaction buffer.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Haeiii Enzyme

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