FGF2 Human, His

FGF2 Human, His

Fibroblast Growth Factor

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  • Name :

    FGF2 Human, His

    Description:

    Fibroblast Growth Factor-Basic Human Recombinant, His

    Prostatropin, FGF-basic, fgf2, Basic FGF, HBGF-2, FGF-2, FGF-b.

    Product # :

    cyt-1270

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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  • description
  • source
  • formulation
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  • More Info

Description

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids (134-288 a.a) and having a molecular mass of 18.1kDa.
The FGF2 is conjugated to 6xHis Tag at N-terminus and is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

FGF2 Human His protein is provided at a concentration of 1mg/ml in PBS Ph-7.2.

Purity

Greater than 90.0% as determined by SDS-PAGE.

More Info

  • Introduction

    FGF2 Human has an important role in cell growth and tissue regeneration. Recent findings show that FGF2 and IGF-1 improve muscle stem cell growth, muscle regeneration, and functional recovery in an animal model of severe muscle loss. FGF2 accelerates wound closure, collagen deposition, and blood vessel formation in diabetic mice. FGF2 forms condensates inside the nucleus thus plays an important role in critical functions in cellular signaling and gene transcription.

  • Synonyms

    Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

  • Physical Appearance

    Sterile Filtered colorless clear solution.

  • Stability

    FGF-2 although stable at 4°C for 1 month, should be stored below -18°C.
    Please prevent freeze thaw cycles.

  • Background

    What is the major role of FGF2 Human His Tag?

    FGF2 Human His Tag enables detection with anti-His antibodies in W.B. or suitable ELISA assays, without the need for an FGF2-specific antibody. FGF2 Human His Tag allows capture on metal-chelate plates or beads for binding studies in assay immobilization.
    FGF2 stimulates growth of embryonic stem cells and induced pluripotent stem cells as well as stimulation of fibroblast and endothelial-cell growth.

    What is the molecular weight / Mw of FGF2 Human His tag?
    FGF2 Human His has a total Mw of 18.1kDa.

    What is the source or expression system of FGF2 Human His?
    E.Coli.

    What is the Purity of FGF2 Human His?
    FGF2 Human His is >90% pure as determined by SDS-PAGE.

    What is the Biological Activity of FGF2 Human His?
    The biological functionality of FGF2 Human His will be determined in the future.

    What is the amino acid sequence of FGF2 Human His?
    FGF2 Human His is composed from 134-288 amino acids.

    What applications can FGF2 Human His be used in?
    FGF2 Human His protein may be suitable for use in Western blot (WB) and ELISA, depending on the assay design.

    What is the endotoxin level for FGF2 Human His?
    The endotoxin level is minimal, FGF2 Human His was purified using conventional chromatography techniques.

    Who supplies recombinant FGF2 Human His that is in stock and available to order?
    ProSpec has the protein in stock and available for immediate delivery.

    Where can i order bulk mg quantities of FGF2 Human His?
    ProSpec supplies bulk mg quantities at affordable prices that meets research and academic budgets.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

FGF2 Human, His

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Frequently asked questions

1. Which company offers Recombinant Growth Factors at competitive prices?

ProSpec offers vast array of Growth Factors such as EGF| IGF1 | Leptin| VEGF and much more at affordable descent prices to meet academic budgets
ProSpec provides High Purity Growth Factors for 30 years, purity exhibits 95% and more as visualized by SDS-PAGE gel.
Growth Factors are in stock for immediate delivery via FedEx which enables scientists and researchers to perform immediate experiments.
Growth Factors are shipped globally using ProSpec’s custom tailored Styrofoam insulated box and premium ice packs that ensure maximum protein stability during transit to your laboratory.

2. Who can produce custom Growth Factor for me?

ProSpec offers custom production of recombinant Growth Factors, managing the entire process from gene design and cloning to protein expression and fermentation. Harvest and cell breakage. Purification using standard chromatography, quality control testing, and formulation. Freeze-drying (lyophilization) is available as an option. For more information contact us at info@prospecbio.com

3. How long does it take to produce a custom Growth Factor

The production time line for a custom recombinant growth factor can take about 5-7 weeks.

4. Where can i find biologically active Growth Factors?

ProSpec offers biologically functional Growth Factors such as Prolactin | Erythropoietin | PDGF-BB which are rigorously tested in-vitro to provide researchers world wide BioActive Growth Factors.

5. What is the activity validation method for Growth Factors?

ProSpec uses cell-based bioassays that measures the biological activity, for example:
Activin-A is validated by Activin-responsive luciferase assay, SMAD2/3 phosphorylation.
CSF, G-CSF, GM-CSF, M-CSF are validated by Cytokine-dependent cell proliferation assay.
CTGF is validated by Fibroblast proliferation or migration assay.
EGF is validated by NIH3T3 or Balb/3T3 proliferation assay.
Epigen (EPGN) is validated by EGFR activation assay, cell proliferation.
Erythropoietin (EPO) is validated by TF-1 or UT-7 erythroid cell proliferation assay.
Fibroblast Growth Factors is validated by NIH3T3 proliferation assay.
IGF-1 and IGF-2 are validated by Cell proliferation assay (MCF-7, Saos-2, BALB/3T3).

6. Is the Growth Factor full length or partial?

The Growth Factor proteins are both full length and partial while majority of them are active mature proteins rather than a full-length precursor. ProSpec active mature proteins exhibits higher expression yields, better stability, Easier purification and equivalent biological activity as full length precursor.

7. Is the Growth Factor monomeric or dimeric form?

This depends on the Growth Factor. ProSpec Growth Factors are supplied in the native biologically active oligomeric state, which can be monomeric, dimeric, trimeric, or even multimeric since because receptor binding and biological activity often depend on correct oligomerization.
The EGF, Epigen, Fibroblast Growth Factors (FGF1, FGF2, FGF7/KGF)
HDGF, IGFBPs (IGFBP-1 to IGFBP-7), IGF2 are usually provided as monomer proteins though Activin-A and myostatin are supplied as homodimers.

8. What is the Growth Factor purity and how was it determined?

The Growth Factors purity ranges from high purity of ≥95% to premium grade ≥97%.
ProSpec uses SDS-PAGE Coomassie Blue Staining as the primary purity method which Visualizes protein bands. Some SDS-PAGE gels are stained with Silver Stain. Other analytical methods include RP-HPLC [ High-resolution purity determination ] , SEC-HPLC for the detections of aggregates and fragments and Western Blot to confirm Growth Factors identity.

9. Is the Growth Factor sequence verified?

The amino acid sequence verification is performed at DNA & protein level.
Sanger DNA Sequencing verifies the expression plasmid having correct coding.
LC-MS is used to verify the molecular weight of the final purified Growth Factor
Prospec also uses Edman N-terminal Sequencing to determine the amino-terminal sequence.

10. What is the endotoxin level in Growth Factors and how do you test LPS ?

ProSpec Growth Factors are tested for endotoxin also named LPS [ lipopolysaccharide ] which is found in the outer membrane of Gram-negative bacteria which can affect cytokine secretion, cell proliferation and stem cell.
ProSpec measures endotoxin using the Limulus Amebocyte Lysate [ LAL ] assay such as Gel-Clot.
ProSpec standard Endotoxin level for cytokines is usually <1 EU/ug.
What Does "EU" Mean? EU = Endotoxin Units

11. Is there host cell protein contamination?

There might be a minor existence of host-cell proteins post chromatography purification and quality-control procedures that greatly minimize these impurities. ProSpec tests host-cell protein levels by SDS-PAGE silver staining, or mass spectrometry.

12. Is the protein tested in vitro, in-vivo cell culture, stem cells, T cells, ELISA, western blot?

PoSpec Growth Factors proteins are usually tested for biological activity and identity, while some proteins are not though supported by literature and customer experience. Interleukins are tested in T-cell, NK-cell, PBMC, stem cell culture. Interferons are tested in Antiviral assays, immune-cell activation, TNFA proteins are tested in Apoptosis, inflammation and immune studies. ProSpec does not conduct in-vivo or animal injections experiments in house or our source.