CKMT1 Human

CKMT1 Human

Creatine Kinases

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  • Name :

    CKMT1 Human

    Description:

    Creatine Kinase Muscle Type-1 Human Recombinant

    Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.

    Product # :

    CKI-271

    Price :

    Quantity :

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.

Source

Pichia Pastoris.

Formulation

CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.

Purity

Greater than 95.0% as determined by
a) Analysis by RP-HPLC
b) Analysis by SDS-PAGE

Biological Activity

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 µmole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.

More Info

  • Introduction

    Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

  • Synonyms

    Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.

  • Physical Appearance

    Sterile Filtered colourless liquid formulation.

  • Stability

    CKMT1 although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Ckmmiti Human

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