CKMM Human Active

CKMM Human Active

Creatine Kinases

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  • Name :

    CKMM Human Active

    Description:

    Creatine Kinase MB Muscle Human Recombinant

    CKMB, CK-MB 

     

    Product # :

    CKI-201

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

CKMM Human Recombinant produced in E. coli is a non-glycosylated disulfide-linked homodimer containing 2 x 381 amino acids and having a total molecular mass of approximately 86.2kDa. CKMM is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

The protein was Lyophilized from a 0.2µm filtered concentrated solution in 20mM Tris, 150mM NaCl and 1mM TCEP, pH 8.0.

Purity

Greater than 95.0% as determined by Analysis by SDS-PAGE.

Biological Activity

The specific activity is > 1,900 pmol/min/μg and was measured by its ability to phosphorylate creatine at room temperature.

More Info

  • Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

  • Stability

    Lyophilized CKMM Active although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CKMM Active should be stored at 4°C between 2-7 days and for future use below -18°C.
    Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized CKMM in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

  • Amino Acid Sequence

    MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K.

  • Background

    CKMM is the muscle-specific form enzyme which catalyzes the reversible transfer of phosphate between ATP and creatine, thus keeping rapid cellular energy homeostasis in skeletal and cardiac muscle. Recombinant CKMM protein is used in studies of muscle metabolism, exercise physiology, muscular dystrophies, biomarker assay development, enzyme kinetics, and the evaluation of compounds affecting energy metabolism and muscle function.

    What is the molecular weight / Mw of CKMM Protein?
    CKMM Protein is a non covalently homodimer having a total Mw of 86.2kDa

    What is the source or expression system of CKMM Protein?
    Escherichia Coli.

    What is the Purity of CKMM Protein?
    CKMM Protein is >95% pure as determined by SDS-PAGE.

    What is the Biological Activity of CKMM Protein?
    The enzymatic activity was determined by its ability to phosphorylate creatine at room temperature. The specific activity is > 1,900 pmol/min/μg.

    What is the amino acid sequence of CKMM Protein?
    MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ K

    What applications can CKMM Protein be used in?
    CKMM Protein can probably be used in western blot, ELISA and Lateral Flow.

    What is the endotoxin level for CKMM Protein?
    The endotoxin level is minimal, CKMM Protein was purified using conventional chromatography techniques.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

CKMM Human Active

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