Carboxypeptidase B Rat

Carboxypeptidase B Rat

Enzymes

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  • Name :

    Carboxypeptidase B Rat

    Description:

    Carboxypeptidase-B Rat Recombinant

    Carboxypeptidase B, Cpb1, Cpb.

    Product # :

    ENZ-475

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    Quantity :

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 31kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.

Source

Escherichia Coli.

Formulation

The protein was lyophilized with 100mM NaCl, mannitol and 20mM Tris pH-7.5.

Purity

Greater than 90% as determined by SDS-PAGE.

Biological Activity

170 units/mg protein.

More Info

  • Introduction

    Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kDa, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.

  • Synonyms

    Carboxypeptidase B, Cpb1, Cpb.

  • Physical Appearance

    Sterile Filtered lyophilized powder.

  • Stability

    Store the lyophilized Carboxypeptidase-B at 4°C. Upon reconstitute the protein should be stored at 4°C for 2 weeks and for future use below -18°C.Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18MΩ-cm H2O or 25mM Tris-HCl pH 7.65 not less than 100µg/ml , which can then be further diluted to other aqueous solutions.

  • Unit Definition

    One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Carboxypeptidase B Rat

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