BMPR1A Human, IgG-His

BMPR1A Human, IgG-His

Bone Morphogenetic Protein

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  • Name :

    BMPR1A Human, IgG-His

    Description:

    Bone Morphogenetic protein Receptor-1A Human Recombinant, IgG-His

    BMPR1A, 10q23del, ACVRLK3, ALK3, CD292, SKR5, Bone Morphogenetic Protein Receptor Type 1A, Bone Morphogenetic Protein Receptor, Type IA, Serine/Threonine-Protein Kinase Receptor R5, Activin Receptor-Like Kinase 3, BMP Type-1A Receptor, EC 2.7.11.30, ALK-3, Bone Morphogenetic Protein Receptor Type-1A, Activin A Receptor, Type II-Like Kinase 3, CD292 Antigen, EC 2.7.1.

    Product # :

    CYT-1005

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  • formulation
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  • More Info

Description

BMPR1A Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 371 amino acids (24-152a.a.) and having a molecular mass of 41.4kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).BMPR1A is fused with a 242 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Formulation

BMPR1A protein solution (1mg/ml) contains Phosphate Buffered Saline (pH7.4) and 10% glycerol.

Purity

Greater than 95.0% as determined by SDS-PAGE.

More Info

  • Introduction

    The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

  • Synonyms

    BMPR1A, 10q23del, ACVRLK3, ALK3, CD292, SKR5, Bone Morphogenetic Protein Receptor Type 1A, Bone Morphogenetic Protein Receptor, Type IA, Serine/Threonine-Protein Kinase Receptor R5, Activin Receptor-Like Kinase 3, BMP Type-1A Receptor, EC 2.7.11.30, ALK-3, Bone Morphogenetic Protein Receptor Type-1A, Activin A Receptor, Type II-Like Kinase 3, CD292 Antigen, EC 2.7.1.

  • Physical Appearance

    Sterile Filtered colorless solution.

  • Stability

    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

  • Amino Acid Sequence

    ADPQNLDSML HGTGMKSDSD QKKSENGVTL APEDTLPFLK CYCSGHCPDD AINNTCITNG HCFAIIEEDD QGETTLASGC MKYEGSDFQC KDSPKAQLRR TIECCRTNLC NQYLQPTLPP VVIGPFFDGS IRLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.

  • Background

    Research Paper on Bone Morphogenetic Protein Receptor-1A Human Recombinant, IgG-His, Monomer, HEK

    Abstract:

    Welcome to the fascinating world of Bone Morphogenetic Protein Receptor-1A Human Recombinant, IgG-His, Monomer (BMPR-1A HR) in Human Embryonic Kidney Cells (HEK). This paper explores the vital role of BMPR-1A HR in cellular responses. As a critical receptor in the transforming growth factor-beta (TGF-β) superfamily, BMPR-1A HR guides cellular differentiation and tissue development. Join us as we uncover the molecular mechanisms behind BMPR-1A HR signaling in HEK cells and explore its synonyms, along with its interactions with key cytokines, including Tumor Necrosis Factor-alpha (TNF-α) and Tumor Necrosis Factor-alpha Superfamily Member 2 (TNFα SF2 or TNFSF2).

    Introduction:

    Step into the captivating realm of BMPR-1A HR! This paper introduces the remarkable BMPR-1A HR and its crucial role in shaping cellular responses. As researchers, we are driven by curiosity to understand how BMPR-1A HR influences cellular behavior and contributes to tissue growth.

    BMPR-1A HR Signaling in HEK Cells:

    Unravel the intricate dance of BMPR-1A HR signaling within HEK cells! We explore the complex process of ligand-receptor binding, which initiates both the canonical SMAD-dependent and non-canonical SMAD-independent pathways, regulating gene transcription, cell proliferation, and differentiation.

    Extensive Description of BMPR-1A HR:

    As researchers, we provide a comprehensive overview of BMPR-1A HR's structural characteristics and unique attributes. With its Monomeric nature and the IgG-His tag, crucial for detection and purification, we delve into the intricacies of BMPR-1A HR.

    Influential Role in Cellular Responses:

    Marvel at BMPR-1A HR's influential role as a critical mediator of cellular responses within HEK cells! Witness how it skillfully modulates cellular differentiation, driving the expression of key differentiation markers like DIF, impacting diverse cellular pathways.

    Interplay with Key Cytokines:

    Uncover the intriguing interactions between BMPR-1A HR and key cytokines like TNF-α and TNFSF2, fostering efficient cellular responses.

    Therapeutic Implications and Tissue Development:

    Explore the potential therapeutic implications of BMPR-1A HR in tissue development. We envision exciting possibilities of utilizing BMPR-1A HR in regenerative medicine, offering hope for enhanced tissue development and repair.

    Conclusion:

    As we conclude our exploration of BMPR-1A HR in HEK cells, we anticipate a promising future where BMPR-1A HR with an IgG-His tag becomes an invaluable tool in biomedical research, promoting human well-being.

    What is the molecular weight/Mw of BMPR1A Protein?
    BMPR1A Protein has a total Mw of 41.4kDa.

    What is the source or expression system of BMPR1A Protein?
    Sf9, Baculovirus cells.

    What is the Purity of BMPR1A Protein?
    BMPR1A Protein is >95% pure as determined by SDS-PAGE.

    What is the Biological Activity of BMPR1A Protein?
    The biological functionality of BMPR1A Protein will be determined in the future.

    What is the amino acid sequence of BMPR1A Protein?
    ADPQNLDSML HGTGMKSDSD QKKSENGVTL APEDTLPFLK CYCSGHCPDD AINNTCITNG HCFAIIEEDD QGETTLASGC MKYEGSDFQC KDSPKAQLRR TIECCRTNLC NQYLQPTLPP VVIGPFFDGS IRLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.

    What applications can BMPR1A Protein be used in?
    BMPR1A Protein can probably be used in western blot, ELISA and Lateral Flow.

    What is the endotoxin level for BMPR1A Protein?
    The endotoxin level is minimal, BMPR1A Protein was purified using conventional chromatography techniques.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Bmpr1A Human Igg His

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Frequently asked questions

1. Which company offers Recombinant cytokines at competitive prices?

ProSpec offers vast array of cytokines such as Interferon-alpha | Interleukin-2 | TNF-alpha | BMP-2 and much more at affordable descent prices to meet academic budgets.
ProSpec provides High Purity cytokines for 30 years, purity exhibits 95% or more by SDS-PAGE gel.
Cytokines are in stock for immediate delivery via FedEx which enables scientists and researchers to perform immediate experiments.
Cytokines are shipped globally using ProSpec’s custom tailored Styrofoam insulated box and premium ice packs that ensure maximum protein stability during transit to your laboratory.

2. Where can i find biologically active cytokines?

ProSpec offers biologically functional cytokines such as Adiponectin | Cardiotrophin | Beta Defensin which are rigorously tested in-vitro to provide researchers world wide BioActive Cytokines.

3. What is the activity validation method for Cytokines?

ProSpec uses cell-based bioassays that measures the biological activity, for example:
Adiponectin is validated using AMPK phosphorylation assay, glucose uptake assay.
Angiopoietin is validated using Endothelial tube formation assay, Tie2 phosphorylation assay.
Apolipoprotein is validated using Lipid-binding assay, receptor-binding assay.
Beta Defensin is validated using Antimicrobial assay.
Betacellulin is validated using Cell proliferation assay (EGFR-positive cells).
Bone Morphogenetic Protein is validated using Alkaline phosphatase induction assay.

4. Is the cytokine full length or partial?

The cytokine proteins are both full length and partial while majority of them are active mature proteins rather than a full-length precursor. ProSpec active mature proteins exhibits higher expression yields, better stability, Easier purification and Equivalent biological activity as full length precursor.

5. Is the cytokine monomeric or dimeric form?

This depends on the cytokine. ProSpec cytokines are supplied in the native biologically active oligomeric state, which can be monomeric, dimeric, trimeric, or even multimeric since because receptor binding and biological activity often depend on correct oligomerization.
The interleukin and interferon protein family are usually provided as monomer proteins though adiponectin and TNFSF8 are supplied as homodimers.

6. What is the cytokine purity and how was it determined?

The cytokine purity ranges from high purity of ≥95% to premium grade ≥97%.
ProSpec uses SDS-PAGE Coomassie Blue Staining as the primary purity method which Visualizes protein bands. Some SDS-PAGE gels are stained with Silver Stain. Other analytical methods include RP-HPLC [ High-resolution purity determination ] , SEC-HPLC for the detections of aggregates and fragments and Western Blot to confirm cytokine identity.

7. Is the cytokine sequence verified?

The amino acid sequence verification is performed at DNA & protein level.
Sanger DNA Sequencing verifies the expression plasmid having correct coding.
LC-MS is used to verify the molecular weight of the final purified cytokine
Prospec also uses Edman N-terminal Sequencing to determine the amino-terminal sequence.

8. What is the endotoxin level in cytokines and how do you test LPS ?

ProSpec cytokines are tested for endotoxin also named LPS [ lipopolysaccharide ] which is found in the outer membrane of Gram-negative bacteria which can affect cytokine secretion, cell proliferation and stem cell.
ProSpec measures endotoxin using the Limulus Amebocyte Lysate [ LAL ] assay such as Gel-Clot.
ProSpec standard Endotoxin level for cytokines is usually <1 EU/ug.
What Does "EU" Mean? EU = Endotoxin Units

9. Is there host cell protein contamination?

There might be a minor existence of host-cell proteins post chromatography purification and quality-control procedures that greatly minimize these impurities. ProSpec tests host-cell protein levels by SDS-PAGE silver staining, or mass spectrometry.

10. Is the protein tested in vitro, in-vivo cell culture, stem cells, T cells, ELISA, western blot?

PoSpec Growth Factors proteins are usually tested for biological activity and identity, while some proteins are not though supported by literature and customer experience. Interleukins are tested in T-cell, NK-cell, PBMC, stem cell culture. Interferons are tested in Antiviral assays, immune-cell activation, TNFA proteins are tested in Apoptosis, inflammation and immune studies. ProSpec does not conduct in-vivo or animal injections experiments in house or our source.