Vascular cell adhesion molecule

Vascular cell adhesion molecule

Vascular cell adhesion molecule

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  • View Data Sheet

    Description:

    Vascular cell adhesion molecule 1 Mouse Recombinant

    V-CAM 1, VCAM-1, CD106, Vcam1, Vcam-1, Vascular cell adhesion protein 1.

    Product # :

    PRO-2257

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    Description

    VCAM1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 682 amino acids (25-698 a.a) and having a molecular mass of 75.4kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions). VCAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    VCAM1 protein solution (0.5mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).

    • Synonyms

      V-CAM 1, VCAM-1, CD106, Vcam1, Vcam-1, Vascular cell adhesion protein 1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      FKIEISPEYK TIAQIGDSMA LTCSTTGCES PLFSWRTQID SPLNAKVRTE GSKSVLTMEP VSFENEHSYL CTATCGSGKL ERSIHVDIYS FPKDPEIQFS GPLEVGKPVT VKCLAPDIYP VYRLEIDLFK GDQLMNRQEF SSEEMTKSLE TKSLEVTFTP VIEDIGKALV CRAKLHIDQI DSTLKERETV KELQVYISPR NTTISVHPST RLQEGGAVTM TCSSEGLPAP EIFWGRKLDN EVLQLLSGNA TLTLIAMRME DSGVYVCEGV NLIGRDKAEV ELVVQEKPFI VDISPGSQVA AQVGDSVVLT CAAIGCDSPS FSWRTQTDSP LNGVVRNEGA KSTLVLSSVG FEDEHSYLCA VTCLQRTLEK RTQVEVYSFP EDPVIKMSGP LVHGRPVTVN CTVPNVYPFD HLEIELLKGE TTLMKKYFLE EMGIKSLETK ILETTFIPTI EDTGKSLVCL ARLHSGEMES EPKQRQSVQP LYVNVAPKET TIWVSPSPIL EEGSPVNLTC SSDGIPAPKI LWSRQLNNGE LQPLSENTTL TFMSTKRDDS GIYVCEGINE AGISRKSVEL IIQVSPKDIQ LTVFPSKSVK EGDTVIISCT CGNVPETWII LKKKAKTGDM VLKSVDGSYT IRQAQLQDAG IYECESKTEV GSQLRSLTLD VKGKEHNKNY FSPELEHHHH HH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Vcam1 Mouse
  • View Data Sheet

    Description:

    Vascular cell adhesion molecule 1 Human Recombinant, sf9

    Vascular Cell Adhesion Molecule 1, CD106 Antigen, INCAM-100 , Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, L1CAM.

    Product # :

    PRO-2367

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    • description
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    • More Info

    Description

    VCAM1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 685 amino acids (25-698 a.a.) and having a molecular mass of 75.6kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). VCAM1 is expressed with a 11 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    VCAM1 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).

    • Synonyms

      Vascular Cell Adhesion Molecule 1, CD106 Antigen, INCAM-100 , Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, CD106, L1CAM.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADPEFFKIET TPESRYLAQI GDSVSLTCST TGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDP EIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDI GKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWS KKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGD SVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVE LYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMT FIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGF PAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFP SESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLR SLTLDVQGRE NNKDYFSPEH HHHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Vcam1 Human Sf9
  • View Data Sheet

    Description:

    Vascular cell adhesion molecule 1 Human Recombinant, HEK

    Vascular cell adhesion protein 1, V-CAM 1, INCAM-100, CD106, VCAM1, L1CAM, MGC99561, DKFZp779G2333.

    Product # :

    PRO-1641

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    Shipped at Room temp

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    • description
    • source
    • formulation
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    • More Info

    Description

    VCAM1 Human Recombinant produced by mammalian expression system in human cells is a single polypeptide chain containing 682 amino acids (25-698). VCAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

    Source

    HEK293 cells.

    Formulation

    VCAM1 was lyophilized from a 0.2 µM filtered solution of 20mM PB, 150mM NaCl, 2mM CaCl2, 2mM MgCl2 and 5%Threhalose, pH 7.2.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).

    • Synonyms

      Vascular cell adhesion protein 1, V-CAM 1, INCAM-100, CD106, VCAM1, L1CAM, MGC99561, DKFZp779G2333.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized VCAM1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VCAM1 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized VCAM1 in 1xPBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    • Amino Acid Sequence

      FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTS
      TLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKP
      ITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIE
      DIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVT
      MTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEV
      ELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTN
      STLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKV
      PSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHID
      DMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGE
      LQPLSENATLTLISTKMEDSGVYLCEGINQIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRE
      NNKDYFSPEVDHHHHHHAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETW
      IILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE
      VDHHHHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Vcam1 Human Hek
  • View Data Sheet

    Description:

    Vascular Cell Adhesion Molecule 1 Human Recombinant

    Vascular cell adhesion protein 1, V-CAM 1, INCAM-100, CD106, VCAM1, L1CAM, MGC99561, DKFZp779G2333.

    Product # :

    PRO-386

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    Quantity :

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    • description
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    • formulation
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    • More Info

    Description

    VCAM1 Human Recombinant is expressed in E. coli containing 674 amino acids (a.a. 25-698) fused to an amino terminal hexahistidine tag, having a total MW of 78.8kDa.

    Source

    Escherichia Coli.

    Formulation

    VCAM1 is supplied in 10mM Tris-HCl (pH 8), 250mM NaCl, 0.5mM DTT, 1.5mM Cysteine, and 50% Glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      VCAM1 is a member of the Ig superfamily. It is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. VCAM-1 contains 6 or 7 immunoglobulin domains, and is expressed on both large and small vessels only after the endothelial cells are stimulated by cytokines. The protein has a number of functions including the regulation of leukocyte migration, leukocyte endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases (artherosclerosis and rheumatoid arthritis).

    • Synonyms

      Vascular cell adhesion protein 1, V-CAM 1, INCAM-100, CD106, VCAM1, L1CAM, MGC99561, DKFZp779G2333.

    • Stability

      Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

    • Applications

      VCAM1 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments.
      The biological activity of this product has not yet been tested.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Vcam1 Human

About VCAM / Vascular Cell Adhesion Molecule:

The vascular cell adhesion molecule is a protein, and it is encoded in humans by a gene known as VCAM1. This functions as a cell adhesion molecule, and proves to be an extremely important process in many forms of life and physiological systems around the world.

VCAM Structure
Looking at the structure of the VCAM-1 gene, we can see six or seven immunoglobulin domains, and the expression on both large and small blood vessels, however this is only after the endothelial cells have been stimulated by cytokines. The molecule is alternatively spliced into two known RNA transcripts, and these transcripts are known to encode different isoforms in humans. The gene product is called a cell surface sialoglycoprotein, which is a type I membrane protein.

Vascular Cell Adhesion Molecule Function
The primary function of the vascular cell adhesion molecule is to mediate the adhesion of lymphocytes, monocytes, eosinophils, and basophils to vascular endothelium. At the same time, the molecule is known to function in leukocyte-endothelial cell signal transduction. There is also a chance, although it is as yet unproven, that it may play a role in the development of atherosclerosis and therefore rheumatoid arthritis.
Similarly, the VCAM-1 protein is an endothelial ligand for VLA-4 (Very Late Antigen-4 or integrin α4β1) of the β1 subfamily of integrins. This type of expression, which we would call VCAM-1 expression, is also known to occur in other cell types such as smooth muscle cells. It has also been shown to interact with EZR and Moesin, and it could exist on the surface of some subpopulations of mesenchymal stem cells (MSC).