U6 Small Nuclear RNA

U6 Small Nuclear RNA

U6 Small Nuclear RNA

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  • View Data Sheet

    Description:

    LSM5 Homolog, U6 Small Nuclear RNA Associated Human Recombinant

    LSM5 homolog, U6 small nuclear RNA associated (S.cerevisiae), YER146W, U6 snRNA-associated Sm-like protein LSm5.

    Product # :

    PRO-795

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    Description

    LSM5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids (1-91 a.a) and having a molecular mass of 12.3kDa.LSM5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    LSM5 protein solution (1mg/ml) containing 20mM Tris-HCl (pH 8.5), 10% glycerol, 0.15M NaCl and 1mM DTT.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

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    • Introduction

      LSM5 also known as LSM5 Homolog, U6 Small Nuclear RNA Associated belongs to the Sm protein family. This family is identified in a variety of organisms based on sequence homology. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length which folds as a loop. In addition, the Sm-like proteins are thought to create a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. Among the diseases associated with LSM5 are spinal muscular atrophy, and muscular atrophy.

    • Synonyms

      LSM5 homolog, U6 small nuclear RNA associated (S.cerevisiae), YER146W, U6 snRNA-associated Sm-like protein LSm5.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMAANATT NPSQLLPLEL VDKCIGSRIH IVMKSDKEIV GTLLGFDDFV NMVLEDVTEF EITPEGRRIT KLDQILLNGN NITMLVPGGE GPEV

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lsm5 Human
  • View Data Sheet

    Description:

    LSM4 Homolog, U6 Small Nuclear RNA Associated Human Recombinant

    U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4, YER112W.

    Product # :

    PRO-065

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    Description

    LSM4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids (1-139 a.a.) and having a molecular mass of 17.5kDa. The LSM4 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The LSM4 solution (0.5 mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

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    • Introduction

      LSM4 (U6 snRNA-associated Sm-like protein) forms donut-shaped heptameric complexes which are involved in various steps of RNA metabolism. LSM4 facilitates RNA protein interactions and structural changes which are essential during ribosomal subunit assembly. LSM4 binds specifically to the 3'-terminal U-tract of U6 snRNA.

    • Synonyms

      U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4, YER112W.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lsm4 Human
  • View Data Sheet

    Description:

    LSM3 Homolog, U6 Small Nuclear RNA Associated Human Recombinant

    LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae), U6 snRNA-associated Sm-like protein LSm3, SMX4, USS2, YLR438C.

    Product # :

    PRO-1060

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    Description

    LSM3 Human Recombinant produced in E. coli is a single polypeptide chain containing 125 amino acids (1-102) and having a molecular mass of 14.3kDa.LSM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The LSM3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 2mM DTT and 20% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      LSM3 is a member of snRNP Sm proteins family which were identified in a large number of organisms based on sequence homology. Sm-like proteins have a Sm sequence motif that holds 2 regions detached by a linker of variable length that fold as a loop. The Sm-like proteins form a stable heteromer found in tri-snRNP particles that are vital for pre-mRNA splicing.

    • Synonyms

      LSM3 homolog U6 small nuclear RNA associated (S. cerevisiae), U6 snRNA-associated Sm-like protein LSm3, SMX4, USS2, YLR438C.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMADDVDQ QQTTNTVEEP LDLIRLSLDE RIYVKMRNDR ELRGRLHAYD QHLNMILGDV EETVTTIEID EETYEEIYKS TKRNIPMLFV RGDGVVLVAP PLRVG

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lsm3 Human
  • View Data Sheet

    Description:

    LSM2 Homolog, U6 Small Nuclear RNA Associated Human Recombinant

    LSM2 homolog U6 small nuclear RNA associated (S. cerevisiae), Small nuclear ribonuclear protein D homolog, chromosome 6 open reading frame 28, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W, Protein G7b, snRNP.

    Product # :

    PRO-1069

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    Description

    LSM2 Human Recombinant produced in E. coli is a single polypeptide chain containing 119 amino acids (1-95) and having a molecular mass of 13.4kDa.LSM2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The LSM2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM Nacl, 5mM DTT and 40% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      LSM2 is a member of the snRNP Sm proteins family. Sm-like proteins are branded in several organisms based on sequence homology with the Sm protein family. Sm-like proteins hold the Sm sequence motif that is made up of 2 regions separated by a linker of variable length which folds as a loop. The Sm-like proteins are believed to create a steady heteromer present in tri-snRNP particles, which are vital for pre-mRNA splicing. LSM2 binds specifically to the 3'-terminal U-tract of U6 snRNA nad takes part in pre-mRNA splicing.

    • Synonyms

      LSM2 homolog U6 small nuclear RNA associated (S. cerevisiae), Small nuclear ribonuclear protein D homolog, chromosome 6 open reading frame 28, snRNP core Sm-like protein Sm-x5, C6orf28, YBL026W, Protein G7b, snRNP.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMLFYSF FKSLVGKDVV VELKNDLSIC GTLHSVDQYL NIKLTDISVT DPEKYPHMLS VKNCFIRGSV VRYVQLPADE VDTQLLQDAA RKEALQQKQ

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lsm2 Human
  • View Data Sheet

    Description:

    LSM12 Homolog, U6 Small Nuclear RNA Associated Human Recombinant

    PNAS-135, Protein LSM12 homolog, LSM12.

    Product # :

    PRO-1329

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    Description

    LSM12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (1-195 a.a.) and having a molecular mass of 24.1kDa.LSM12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    LSM12 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl, 10% glycerol and 1mM DTT.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      LSM12 Homolog, U6 Small Nuclear RNA Associated (LSM12) is a member of the snRNP Sm proteins family. Sm-like proteins were detected in various organisms based on sequence homology with the Sm protein family. Sm-like proteins comprise the Sm sequence motif, which consists of two regions parted by a linker of variable length which folds as a loop. The Sm-like proteins are assumed to create a stable heteromer present in tri-snRNP particles, which are significant for pre-mRNA splicing.

    • Synonyms

      PNAS-135, Protein LSM12 homolog, LSM12.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMAAPPGE YFSVGSQVSC RTCQEQRLQG EVVAFDYQSK MLALKCPSSS GKPNHADILL INLQYVSEVE IINDRTETPP PLASLNVSKL ASKARTEKEE KLSQAYAISA GVSLEGQQLF QTIHKTIKDC KWQEKNIVVM EEVVITPPYQ VENCKGKEGS ALSHVRKIVE KHFRDVESQK ILQRSQAQQP QKEAALSS.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lsm12 Human
  • View Data Sheet

    Description:

    LSM1 Homolog, U6 Small Nuclear RNA Associated Human Recombinant

    U6 snRNA-associated Sm-like protein LSm1, Cancer-associated Sm-like, Small nuclear ribonuclear CaSm, LSM1, CASM, YJL124C.

    Product # :

    PRO-259

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    Description

    LSM1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 153 amino acids (1-133 a.a.) and having a molecular mass of 17.3kDa. The LSM1 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The LSM1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 10% glycerol and 0.1M NaCl.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      U6 snRNA-associated Sm-like protein LSm1 (LSM1) has a role in replication-dependent histone mRNA degradation and binds specifically to the 3''-terminal U-tract of U6 snRNA. Sm-like proteins include the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop. The LSM1 protein facilitates RNA protein interactions and structural modifications which are required during ribosomal subunit assembly. The Sm-like proteins are believed to form a stable heteromer present in tri-snRNP particles, which are vital for pre-mRNA splicing. LSM1 is overexpressed in pancreatic cancer as well as in several breast cancer cell lines.

    • Synonyms

      U6 snRNA-associated Sm-like protein LSm1, Cancer-associated Sm-like, Small nuclear ribonuclear CaSm, LSM1, CASM, YJL124C.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MNYMPGTASL IEDIDKKHLV LLRDGRTLIG FLRSIDQFAN LVLHQTVERI HVGKKYGDIP RGIFVVRGEN VVLLGEIDLE KESDTPLQQV SIEEILEEQR VEQQTKLEAE KLKVQALKDR GLSIPRADTL DEY.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lsm1 Human

About LSM / U6 Small Nuclear RNA:

Small Nuclear RNA is a category of Small RNA molecules located within the splicing speckles and the Cajal bodies of the cell nucleus in all eukaryotic animals. On the other hand, the U6 Small Nuclear RNA is the nucleolar snRNA element of small nuclear ribonucleoprotein. The U6 snRNA, also known as LSM, is an RNA- Protein compound that combines with other small nuclear ribonucleoproteins, unmodified precursor messenger RNA, also known as pre-mRNA, plus other proteins to gather a spliceosome. A spliceosome is a big RNA- protein molecular compound that plays a significant role in catalyzing the excision of introns from pre-mRNA.

LSM Structure
The U6 small nuclear RNA is made of many protein elements. And apart from that, this spliceosome has five uridine-rich small nuclear RNA that go through an elaborate series of conformational transitions to accurately identify the splice sites and catalyze intron removal.
In the mammalian cells, there are six main molecular species that are filled with proteins, creating small nuclear ribonucleoprotein molecules in precursor messenger RNA splicing as part of the spliceosome.

U6 Small Nuclear RNA Interactions
According to many years of genetic and biochemical data, this protein forms most of the catalytic core of the spliceosome and is highly dynamic. It interacts with three small nuclear RNAs, namely the precursor messenger RNA substrate and more than twenty-five more protein partners during the splicing cycle.

LSM Function
The U6 small nuclear RNA is vital for and might participate in the catalysis of precursor messenger RNA splicing. Some nucleotides needed for U6 function and spicing have been identified in numerous mutation analyses in many systems.