TNF receptor-Associated Factor

TNF receptor-Associated Factor

TNF receptor-Associated Factor

Name

Description

Product #

Price

Quantity

Shipping Method

  • Name :

    TRAF1 Human

    Description:

    TNF receptor-Associated Factor 1 Human Recombinant

    TRAF-1, EBI6, Epstein-Barr virus-induced protein 6, TNF Receptor-Associated Factor 1, MGC:10353.

    Product # :

    PRO-834

    Price :

    Quantity :

    Shipping Method :

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    • description
    • source
    • formulation
    • purity
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    Description

    TRAF1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids (266-416 a.a.) and having a molecular mass of 19.5 kDa. TRAF1 protein is fused to a 21 amino acid His tag at N-terminus and is purified by standard chromatography.

    Source

    Escherichia Coli.

    Formulation

    TRAF1 Human solution containing 20mM Tris-HCl pH-8, 0.1M NaCl, 1mM DTT & 20% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      TRAF1 is an adapter protein and signal transducer that connects members of the TNFR family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. TRAF1 mediates activation of NF-kappa-B and JNK and participates in apoptosis. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.

    • Synonyms

      TRAF-1, EBI6, Epstein-Barr virus-induced protein 6, TNF Receptor-Associated Factor 1, MGC:10353.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MDGTFLWKIT NVTRRCHESA CGRTVSLFSP AFYTAKYGYK LCLRLYLNGD GTGKRTHLSL FIVIMRGEYD ALLPWPFRNK VTFMLLDQNN REHAIDAFRP DLSSASFQRP QSETNVASGC PLFFPLSKLQ SPKHAYVKDD TMFLKCIVET ST.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Traf1 Human

  • Name :

    TANK Human (1-119)

    Description:

    TRAF Family Member-Associated NFKB Activator Human Recombinant (1-119 a.a.)

    TRAF2, I-TRAF, TANK, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, ITRAF.

    Product # :

    PRO-685

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    TANK produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids (1-119 a.a.) and having a molecular mass of 13.6 kDa.TANK is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The TANK protein solution contains 20mM Tris, pH-8.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TRAF2 protein is related with transduce signals from members of the TNFR superfamily. TRAF2 is located in the cytoplasm binds to TRAF1, TRAF2, or TRAF3, thus inhibiting TRAF function by sequestering the TRAFs in a suppressed state in the cytoplasm. Overexpression of TANK inhibits TRAF2-mediated NF-Kappa-B activation signaled by CD40 and both TNF receptors and inhibits LMP1-mediated NF-kappa-B activation by blocking the association of TRAF2 with LMP1. TRAF2 is necessary for the cellular response to TNF-alpha by connecting upstream signalling molecules to the IKKs and p65.

    • Synonyms

      TRAF2, I-TRAF, TANK, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, ITRAF.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MDKNIGEQLN KAYEAFRQAC MDRDSAVKEL QQKTENYEQR IREQQEQLSL QQTIIDKLKS QLLLVNSTQD NNYGCVPLLE DSETRKNNLT LDQPQDKVIS GIAREKLPKV DIASAESSI.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Traf2 Human

About TRAF / TNF receptor-Associated Factor:

TRAFs, or TNF receptor-associated factors, are a family of proteins that are mainly engaged in the regulation of inflammation, antiviral responses, and apoptosis. There have been seven of these found in mammals: TRAF1, TRAF2, TRAF3, TRAF4, TRAF5, TRAF6, and TRAF7. All of them, except for TRAF7, have a relatively conserved secondary structure.

TRAF Function
Each TRAF protein has its own function. The function of TRAF1 and TRAF2, for instance, is to form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. This complex also interacts with IAP and mediates the anti-apoptotic signals from TNF receptors. Meanwhile, TRAF3 participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response and is critical in the lymphotoxin-beta receptor signaling complex.

TNF receptor-Associated Factor Mechanism
The mechanisms for the TRAF proteins are still undergoing study and not all of them have been fully discovered.

TRAF Interactions
Each of the TRAF proteins has its own series of interactions. TRAF1 interacts with TRAF2, as well as CD40 (CD40 Molecule), BIRC3 (Baculoviral IAP Repeat Containing 3), CASP8 (Caspase 8), and SRC (SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase). Meanwhile, TRAF3 interacts with CD40 (CD40 Molecule), TBK1 (TANK Binding Kinase 1), MAVS (Mitochondrial Antiviral Signaling Protein), IKBKE (Inhibitor of Nuclear Factor Kappa B Kinase Subunit Epsilon), and TICAM1 (Toll Like Receptor Adaptor Molecule 1).

TNF receptor-Associated Factor Structure
The TRAF domain is composed of 7–8 anti-parallel β-strand folds followed by a coiled-coil region that mediates protein interactions, usually forms mushroom-like trimeric structures in solution. This structure applies to all TRAF proteins.