TIMP

TIMP

TIMP

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  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 4 Human Recombinant

    TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor Of Metalloproteinases 4, Tissue Inhibitor Of Metalloproteinase 4, TIMP-4, Metalloproteinase Inhibitor 4.

    Product # :

    ENZ-976

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    Description

    TIMP4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 204 amino acids (30-224 a.a.) and having a molecular mass of 23.5kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). TIMP4 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    TIMP4 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Metalloproteinase inhibitor 4 (TIMP4) is a member of a family of secreted proteins which regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases. The four known members of this family are TIMP1, 2, 3 and 4. TIMP4 is produced by various tissues, particularly the brain, heart, ovary and skeletal muscle. Limited studies have shown that the TIMP4 protein has a tumor suppressive effect against Wilm92s tumor, exhibiting a negative correlation with glioma malignancy and is found in breast carcinoma cells.

    • Synonyms

      TIMP Metallopeptidase Inhibitor 4, Tissue Inhibitor Of Metalloproteinases 4, Tissue Inhibitor Of Metalloproteinase 4, TIMP-4, Metalloproteinase Inhibitor 4.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADPCSCAPAH PQQHICHSAL VIRAKISSEK VVPASADPAD TEKMLRYEIK QIKMFKGFEK VKDVQYIYTP FDSSLCGVKL EANSQKQYLL TGQVLSDGKV FIHLCNYIEP WEDLSLVQRE SLNHHYHLNC GCQITTCYTV PCTISAPNEC LWTDWLLERK LYGYQAQHYV CMKHVDGTCS WYRGHLPLRK EFVDIVQPHH HHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp4 Human
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 2 Human Recombinant, Sf9

    TIMP Metallopeptidase Inhibitor 2, Tissue Inhibitor Of Metalloproteinases 2, CSC-21K, Tissue Inhibitor Of Metalloproteinase 2, TIMP-2, DDC8, Metalloproteinase inhibitor 2.

    Product # :

    ENZ-936

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    Description

    TIMP2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 200 amino acids (27-220a.a.) and having a molecular mass of 22.5kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). TIMP2 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    TIMP2 protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.

    • Synonyms

      TIMP Metallopeptidase Inhibitor 2, Tissue Inhibitor Of Metalloproteinases 2, CSC-21K, Tissue Inhibitor Of Metalloproteinase 2, TIMP-2, DDC8, Metalloproteinase inhibitor 2.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDPHHHHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp2 Human Sf9
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 2 Human Recombinant, His Tag

    TIMP metallopeptidase inhibitor 2, metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP-2, CSC-21K.

    Product # :

    ENZ-646

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    Description

    TIMP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 232 amino acids (27-220) and having a molecular mass of 26.1 kDa.TIMP2 is fused to a 38 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The TIMP2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.

    • Synonyms

      TIMP metallopeptidase inhibitor 2, metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP-2, CSC-21K.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHM CS CSPVHPQQAF CNADVVIRAK AVSEKEVDSG NDIYGNPIKR IQYEIKQIKM FKGPEKDIEF IYTAPSSAVC GVSLDVGGKK EYLIAGKAEG DGKMHITLCD FIVPWDTLST TQKKSLNHRY QMGCECKITR CPMIPCYISS PDECLWMDWV TEKNINGHQA KFFACIKRSD GSCAWYRGAA PPKQEFLDIE DP

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp2 Human
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 2 Human Recombinant, HEK

    TIMP metallopeptidase inhibitor 2, CSC-21K, tissue inhibitor of metalloproteinase 2, TIMP-2, metalloproteinase inhibitor 2.

    Product # :

    ENZ-120

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    Description

    TIMP2 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-2 (amino acids Cys27-Pro220) and is fused to a polyhistidine tag at the C-terminus.

    Source

    HEK293 Cells.

    Formulation

    The TIMP2 protein was lyophilized after extensive dialysis against PBS.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    The IC50 of 3 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.

    More Info

    • Introduction

      TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMPfamily members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.

    • Synonyms

      TIMP metallopeptidase inhibitor 2, CSC-21K, tissue inhibitor of metalloproteinase 2, TIMP-2, metalloproteinase inhibitor 2.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5) not less than 100µg/ml.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp2 Human Hek
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 2 Human Recombinant

    Fibroblast growth factor 17, FGF-17, FGF17, FGF-13, HH20.

    Product # :

    ENZ-782

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    Description

    TIMP2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 194 amino acids and having a molecular mass of 21.8kDa.

    Source

    Escherichia Coli.

    Formulation

    TIMP2 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 3 % trehalose.

    Purity

    Greater than 95.0% as determined by: (a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

    Biological Activity

    Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.

    More Info

    • Introduction

      TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.

    • Synonyms

      Fibroblast growth factor 17, FGF-17, FGF17, FGF-13, HH20.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized TIMP2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    • Amino Acid Sequence

      CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp 2 Human
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 1 Rat Recombinant

    Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1.

    Product # :

    ENZ-922

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    Description

    TIMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (24-217 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 200 amino acids and having a molecular mass of 22.3kDa.TIMP1 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    TIMP1 Ligand protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells.
      The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds.
      TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.
      Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.

    • Synonyms

      Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      CSCAPTHPQT AFCNSDLVIR AKFMGSPEII ETTLYQRYEI KMTKMLKGFD AVGNATGFRF AYTPAMESLC GYVHKSQNRS EEFLIAGRLR NGNLHITACS FLVPWHNLSP AQQKAFVKTY SAGCGVCTVF PCSAIPCKLE SDSHCLWTDQ ILMGSEKGYQ SDHFACLPRN PDLCTWQYLG VSMTRSLPLA KAEAHHHHHH

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp1 Rat
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 1 Mouse Recombinant

    Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1.

    Product # :

    ENZ-924

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    Description

    TIMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (25-205 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 187 amino acids and having a molecular mass of 21kDa.TIMP1 is purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    TIMP1 Ligand protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells.
      The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds.
      TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.
      Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.

    • Synonyms

      Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      CSCAPPHPQT AFCNSDLVIR AKFMGSPEIN ETTLYQRYKI KMTKMLKGFK AVGNAADIRY AYTPVMESLC GYAHKSQNRS EEFLITGRLR NGNLHISACS FLVPWRTLSP AQQRAFSKTY SAGCGVCTVF PCLSIPCKLE SDTHCLWTDQ VLVGSEDYQS RHFACLPRNP GLCTWRSLGA RHHHHHH

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp1 Mouse
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 1 Human Recombinant, Sf9

    TIMP Metallopeptidase Inhibitor 1, TIMP, EPA, Tissue Inhibitor Of Metalloproteinases 1, Fibroblast Collagenase Inhibitor, Erythroid-Potentiating Activity, Collagenase Inhibitor, TIMP-1, CLGI, Tissue Inhibitor Of Metalloproteinase 1 (Erythroid Potentiating Activity, Collagenase Inhibitor), Erythroid Potentiating Activity, Metalloproteinase Inhibitor 1, EPO, HCI, Metalloproteinase inhibitor 1.

    Product # :

    ENZ-876

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    Description

    TIMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 190 amino acids (24-207a.a.) and having a molecular mass of 21.5kDa. TIMP1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    TIMP1 protein solution (0.2mg/ml) contains phosphate buffered saline (pH7.4).

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells. The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds. TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.
      Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.

    • Synonyms

      TIMP Metallopeptidase Inhibitor 1, TIMP, EPA, Tissue Inhibitor Of Metalloproteinases 1, Fibroblast Collagenase Inhibitor, Erythroid-Potentiating Activity, Collagenase Inhibitor, TIMP-1, CLGI, Tissue Inhibitor Of Metalloproteinase 1 (Erythroid Potentiating Activity, Collagenase Inhibitor), Erythroid Potentiating Activity, Metalloproteinase Inhibitor 1, EPO, HCI, Metalloproteinase inhibitor 1.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp1 Human Sf9
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 1 Human Recombinant, HEK

    Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.

    Product # :

    ENZ-508

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    • description
    • source
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    • More Info

    Description

    TIMP1 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-1 (amino acids Cys24-Ala207) and fused to a polyhistidine tag at the C-terminus.

    Source

    HEK293 Cells.

    Formulation

    The TIMP1 protein was lyophilized after extensive dialysis against PBS.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    The IC50 of 2.5-4 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.

    More Info

    • Introduction

      TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells. The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds. TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones. Increased TIMP1 levels are connecte

    • Synonyms

      Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized TIMP1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Timp1 Hek
  • View Data Sheet

    Description:

    Tissue Inhibitor of Metalloprotease 1 Human Recombinant

    Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.

    Product # :

    ENZ-442

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    Description

    TIMP1 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 184 amino acids fragment (24-207) having a total molecular mass of 25.21kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The TIMP1 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    TIMP1 protein is supplied in 25mM Sodium acetate pH4.8 and 50% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells.
      The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds.
      TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.
      Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.

    • Synonyms

      Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

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    Timp1 Human

About TIMP / Tissue Inhibitor of Metalloprotease:

TIMP, or tissue inhibitor of metalloproteinase, is a family of protease inhibitors, which regulate matrix metalloproteinases or MMPs.
There are four TIMPs, known as: TIMP-1, TIMP-2, TIMP-3, TIMP-4.

TIMP Function
TIMPS are specific protease inhibitors, which regulate and control MMPs. MMPs are zinc-dependent endopeptidases, which play an instrumental role in extracellular matrix degradation and subsequently impact multiple processes, including: Tissue repair, Cirrhosis, Wound healing, Tumour invasion and metastasis, Morphogenesis, angiogenesis.
TIMPs play a crucial role in maintaining good health, and an imbalance between TIMPs and MMPs has been linked to a host of medical issues, including: Rheumatoid and osteoarthritis, Cardiovascular disease and degradation of the aortic wall, Cancer metastasis and Endometriosis.
In addition to their inhibiting role, TIMPs can also promote cell development across a broad spectrum of cell types, and studies suggest that TIMP-1 can have anti-apoptotic effects. Apoptosis is a molecular process, which contributes to the death of cancerous cells.

Tissue Inhibitor of Metalloprotease Mechanism
The TIMP family, which comprises four members, contains three secreted proteins, TIMP-1, TIMP-2 and TIMP-4, and one membrane-bound protein, TIMP-4. TIMPs bind with MMPs, prohibiting access to the catalytic domain. As TIMP-3 is a membrane-bound protein, it can only target the extracellular matrix. TIMPs can be expressed from several different tissues, and expression is controlled by hormones and cytokines. In healthy individuals, the expression of MMPs should be minimal.

TIMP Structure
TIMPs in human beings have two key domains: an N-terminal and a C-terminal. The N-terminal comprises up to 125 amino acids, while the C-terminal comprises around 65 residues. The four TIMP family members are approximately 40% identical in sequence to each other. TIMP-2 and TIMP-4 are the most similar, sharing around 50% of the same structure.