Surfeit

Surfeit

Surfeit

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  • View Data Sheet

    Description:

    Surfeit-2 Human Recombinant

    Surfeit 2, SURF-2, SURF2, Surfeit locus protein 2, Surfeit-2.

    Product # :

    PRO-1470

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    • description
    • source
    • formulation
    • purity
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    Description

    SURF2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 279 amino acids (1-256) and having a molecular mass of 32 kDa. SURF2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The SURF2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Surfeit-2 (SURF2) is a member of the SURF2 family. SURF2 interacts with beta-1, 4-Gal-T3, uPAR and WDR20. SURF2 is located in the surfeit gene cluster, a group of extremely tightly linked genes that don't share sequence similarity. The SURF2 gene maps to human chromosome 9q34.2 and shares a bidirectional promoter with SURF1 that is found on the opposite strand. The intergenic region between the SURF1 and SURF2 genes is expected to have bidirectional promoter activity, as is found in mice.

    • Synonyms

      Surfeit 2, SURF-2, SURF2, Surfeit locus protein 2, Surfeit-2.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSELPGD VRAFLREHPS LRLQTDARKV RCILTGHELP CRLPELQVYT RGKKYQRLVR ASPAFDYAEF EPHIVPSTKN PHQLFCKLTL RHINKCPEHV LRHTQGRRYQ RALCKYEECQ KQGVEYVPAC LVHRRRRRED QMDGDGPRPR EAFWEPTSSD EGGAASDDSM TDLYPPELFT RKDLGSTEDG DGTDDFLTDK EDEKAKPPRE KATDEGRRET TVYRGLVQKR GKKQLGSLKK KFKSHHRKPK SFSSCKQPG.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Surf2 Human
  • View Data Sheet

    Description:

    Surfeit-1 Human Recombinant

    SURF1, SURF-1, Surfeit locus protein 1, Surfeit 1.

    Product # :

    PRO-764

    Price :

    Quantity :

    Shipping Method :

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    • description
    • source
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    • More Info

    Description

    Surfeit-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 215 amino acids (80-273 a.a.) and having a molecular mass of 24.3 kDa. The Surfeit-1 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The Surfeit-1 solution contains 20 mM Tris-HCl buffer (pH 8.0), 0.1M Nacl, 1mM DTT and 20% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      SURF1 plays a role in the biogenesis of the COX complex. SURF1 is found in the inner mitochondrial membrane and takes part in the biogenesis of the cytochrome c oxidase complex. SURF1 is shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this SURF1 cause Leigh syndrome, a rigorous neurological disorder that is generally associated with systemic cytochrome c oxidase deficiency.

    • Synonyms

      SURF1, SURF-1, Surfeit locus protein 1, Surfeit 1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MQVQRRKWKL NLIAELESRV LAEPVPLPAD PMELKNLEYR PVKVRGCFDH SKELYMMPRT MVDPVREARE GGLISSSTQS GAYVVTPFHC TDLGVTILVN RGFVPRKKVN PETRQKGQIE GEVDLIGMVR LTETRQPFVP ENNPERNHWH YRDLEAMARI TGAEPIFIDA NFQSTVPGGP IGGQTRVTLR NEHLQ.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Surf1 Human

About SURF / Surfeit:

Surfeit locus protein 1 (SURF1) is a multi-pass protein believed to be used in the mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complexes (also known as Complex IV, COX, and/or MITRAC complex). It thus plays a role in the regulation of the cytochrome c oxidase assembly.
Mutations in SURF1 are strongly linked with Leigh syndrome. This in turn is linked with systemic cytochrome c oxidase (complex IV) deficiency, and Charcot-Marie-Tooth disease 4K (CMT4K).

SURF Function
The SURF1 protein is found in the inner mitochondrial membrane. It is believed to play a role in the biogenesis of Complex IV. Complex IV plays a vital role in oxidative phosphorylation. It attracts electrons created through previous steps in oxidative phosphorylation and protons from inside the mitochondrion. Using these, it converts oxygen to water.
Additionally, Complex IV sends protons across the specialized membrane inside the mitochondrion. This creates energy which is then converted into adenosine triphosphate (ATP). This is the form of energy mainly used for cellular processes.

Surfeit Structure
The SURF1 gene forms part of the surfeit gene cluster. These genes are very tightly linked but do not have similar sequences. The SURF1 gene is located in position 34.2 of the q arm of chromosome 9 (9q34.2). It has 9 exons. The SURF1 protein is a 33.3 kDa protein. It consists of 300 amino acids.

SURF Interactions
SURF1 has a total of 11 binary protein-protein interactions. Of these, 8 are co-complex interactions. Genes known to interact with SURF1 include:
COA1
COA3
COX6C
COX14
PTGES3
SLC25A5