sRAGE

sRAGE

sRAGE

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    Description:

    Advanced Glycosylation End Product-Specific Receptor Human Recombinant, Sf9

    Advanced Glycosylation End-Product Specific Receptor, Advanced Glycosylation End Product-Specific Receptor, RAGE, Receptor for Advanced Glycation End-Products Variant 20, Receptor for Advanced Glycosylation End Products, Receptor for Advanced Glycation End-Products, RAGE Isoform NtRAGE-Delta, RAGE Isoform SRAGE-Delta, SCARJ1.

    Product # :

    PRO-2645

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    Description

    RAGE Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 561 amino acids (24-342 a.a) and having a molecular mass of 61.2kDa. RAGE is fused to a 242 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    The RAGE solution (0.25mg/1ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    Biological Activity

    Measured by the ability of the binding activity in a functional ELISA. The ED50 range ≤15ug/ml.

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    • Introduction

      Advanced Glycosylation End Product-Specific Receptor or RAGE is an immunoglobulin, part of the immunoglobulin transmembrane proteins family. RAGE moderates interactions between advanced glycosylation end products, also known as AGE. These signaling has a crucial part in the regulation of TNFalpha,oxidative stress, endothelial dysfunction in type 2 diabetesproduction and expression. RAGE helps the translocation of ABPP (amyloid-beta peptide) through the membrane of cells to the intracellular area in the cortical neurons.

    • Synonyms

      Advanced Glycosylation End-Product Specific Receptor, Advanced Glycosylation End Product-Specific Receptor, RAGE, Receptor for Advanced Glycation End-Products Variant 20, Receptor for Advanced Glycosylation End Products, Receptor for Advanced Glycation End-Products, RAGE Isoform NtRAGE-Delta, RAGE Isoform SRAGE-Delta, SCARJ1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADLQNITARI GEPLVLKCKG APKKPPQRLE WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LALEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEA HNHYTQKSLS LSPGKHHHHH H.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rage Protein
  • View Data Sheet

    Description:

    Advanced Glycosylation End Product-Specific Receptor Mouse Recombinant

    Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER, SRAGE, RAGE, MGC22357.

    Product # :

    PRO-2781

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    Shipped at Room temp

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    Description

    sRAGE Mouse Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain containing 317 amino acids (Gly23–Asp333) and having a molecular mass of 34.0kDa. sRAGE Mouse is fused to a 6 a.a his tag at C-Terminus and is purified by proprietary chromatographic techniques.

    Source

    HEK 293.

    Formulation

    The filtered (0.4µm) concentrated protein solution was lyophilized with PBS, PH 7.4.

    Purity

    Greater than 95% as determined by SDS-PAGE.

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    • Synonyms

      Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER, SRAGE, RAGE, MGC22357.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.

    • Solubility

      It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.

    • Amino Acid Sequence

      GQNITARIGE PLVLSCKGAP KKPPQQLEWK LNTGRTEAWK VLSPQGGPWD SVARILPNGS LLLPATGIVD EGTFRCRATN RRGKEVKSNY RVRVYQIPGK PEIVDPASEL TASVPNKVGT CVSEGSYPAG TLSWHLDGKL LIPDGKETLV KEETRRHPET GLFTLRSELT VIPTQGGTHP TFSCSFSLGL PRRRPLNTAP IQLRVREPGP PEGIQLLVEP EGGIVAPGGT VTLTCAISAQ PPPQVHWIKD GAPLPLAPSP VLLLPEVGHE DEGTYSCVAT HPSHGPQESP PVSIRVTETG DEGPAEGEGL DHHHHHH.

    • Background

      The soluble receptor for advanced glycation end products, sRAGE, is a multifunctional protein known for its involvement in diverse physiological processes, including inflammation, aging, and chronic diseases. Research using mouse models has been instrumental in unraveling the complexities of sRAGE biology and its implications for health and disease. This study aims to provide a comprehensive exploration of sRAGE in mouse physiology, shedding light on its various functions and potential applications in understanding aging and disease mechanisms.

      The primary objective of this research is to elucidate the impact of sRAGE in mouse models on aging processes. In vivo experiments utilizing genetically modified mice with altered sRAGE expression will be conducted to investigate how sRAGE influences the aging process, including effects on tissue homeostasis, oxidative stress, and longevity. Understanding these mechanisms is fundamental for deciphering the role of sRAGE in age-related diseases.

      The second objective is to assess the clinical relevance of sRAGE in mouse models of chronic diseases. Mouse models of diseases such as diabetes, Alzheimer's disease, and cancer will be employed to explore how sRAGE modulation affects disease progression, inflammation, and tissue damage. These investigations may provide valuable insights into potential therapeutic strategies targeting sRAGE in various chronic diseases.

      The third objective is to explore the broader implications of sRAGE in mouse physiology, including its effects on immunity, tissue repair, and metabolic regulation. Research will investigate its roles in immune cell function, wound healing, and glucose homeostasis. Understanding the multifaceted properties of sRAGE in mouse models may open new avenues for therapeutic interventions in various health and disease contexts.

      By delving into the diverse functions of sRAGE in mouse physiology, this research aims to expand our knowledge of its physiological roles and clinical applications. The findings may contribute to the development of targeted interventions for age-related diseases and chronic conditions influenced by sRAGE.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Srage Mouse
  • View Data Sheet

    Description:

    Advanced Glycosylation End Product-Specific Receptor Human Recombinant

    Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER, SRAGE, RAGE, MGC22357.

    Product # :

    PRO-600

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    Description

    sRAGE Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 339 amino acids and having a molecular mass of 36.5 kDa. The Human sRAGE is fused to a 14 a.a. His tag at N-Terminus.The Human sRAGE is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The sterile filtered (0.4 µm) concentrated (0.5mg/ml) protein solution was lyophilized with 30mM acetate buffer pH-4.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      sRAGE is a member of the immunoglobulin superfamily of cell surface molecules. sRAGE is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. sRAGE lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Alternative splicing results in two transcript variants encoding different isoforms. sRAGE mediates interactions of nonenzymatic glycosylated proteins which accumulate in vascular tissue during aging & at an increasing rate in diabetes. sRAGE is a receptor for amyloid beta peptide.

    • Synonyms

      Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER, SRAGE, RAGE, MGC22357.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.

    • Solubility

      Add 0.1M Acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely.

    • Amino Acid Sequence

      MRGSHHHHHH GMASAQNITA RIGEPLVLKC KGAPKKPPQR LEWKLNTGRT EAWKVLSPQG GGPWDSVARV LPNGSLFLPAV GIQDEGIFRCQ AMNRNGKETKS NYRVRVYQIP GKPEIVDSASE LTAGVPNKVG TCVSEGSYPA GTLSWHLDGKPL VPNEKGVSVK EQTRRHPETG LFTLQSELMV TPARGGDPRP TFSCSFSPGL PRHRALRTAP IQPRVWEPVPL EEVQLVVEPE GGAVAPGGTV TLTCEVPAQP SPQIHWMKDGVP LPLPPSPVLI LPEIGPQDQG TYSCVATHSS HGPQESRAVS ISIIEPGEEG PTAGEGFDKV REAEDSPQHM

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Srage Human

About sRAGE / Advanced Glycosylation End Product-Specific Receptor:

RAGE stands for Receptor for Advanced Glycation Endproducts. They’re sometimes referred to as AGER. It is a 35 kilodalton transmembrane receptor which belongs to the immunoglobulin super family. This was first mapped by Neeper and colleagues back in 1992. It was given its name because of its ability to bind advanced glycation endproducts, such as glycoproteins; the glycine of these have been modified -- non-enzymatically -- through the Maillard reaction.

sRAGE Function
RAGE is commonly characterized as a pattern recognition reception. This is because it can perform an inflammatory function in innate immunity, and also because of its ability to detect a class of ligands through a common structural motif. Researchers have found that isoforms of the RAGE protein are typically hypothesized to counteract the full-length receptor. There is hope in the medical community that they can be used to develop a cure against RAGE-associated diseases.

Advanced Glycosylation End Product-Specific Receptor Structure
In terms of structure, RAGE exists in two different forms. There’s the membrane-found form, which is referred to as mRAGE, and then there’s the soluble form, which is referred to as sRAGE. While mRAGE has three domains, sRAGE only has one -- the extracellular domain. Researchers have noted that sRAGE will be the product of either alternative splicing or a result of proteolytic cleavage of mRAGE. The full receptor will have the five domains. These are the cytosolic domain, which functions to signal transduction; the transmembrane domain, which helps to anchor the receptor in the cell membrane; the variable domain, which helps to bind the RAGE ligands; and then two constant domains.