SH2 Domain

SH2 Domain

SH2 Domain

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  • View Data Sheet

    Description:

    SH2 domain containing 1B Human Recombinant

    SH2 Domain Containing 1B, EWS/FLI1-Activated Transcript 2, EAT-2, EAT2, SH2 Domain-Containing Molecule EAT2, SH2 Domain-Containing Protein 1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2.

    Product # :

    PRO-2470

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    Description

    SH2D1B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 141 amino acids (1-132a.a.) and having a molecular mass of 16.4kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SH2D1B is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    SH2D1B protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 20% glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

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    • Introduction

      SH2 domain-containing protein 1B, also known as SH2D1B activates NK cells. SH2D1B participates in controlling signal transduction through at least 4 receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. Two isoforms of the human SH2D1B are produced by alternative splicing. Superior Mesenteric Artery Syndrome is one of the diseases which is associated with SH2D1B.

    • Synonyms

      SH2 Domain Containing 1B, EWS/FLI1-Activated Transcript 2, EAT-2, EAT2, SH2 Domain-Containing Molecule EAT2, SH2 Domain-Containing Protein 1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADPMDLPYYH GRLTKQDCET LLLKEGVDGN FLLRDSESIP GVLCLCVSFK NIVYTYRIFR EKHGYYRIQT AEGSPKQVFP SLKELISKFE KPNQGMVVHL LKPIKRTSPS LRWRGLKLEL ETFVNSNSDY VDVLPHHHHH H.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sh2D1B Human
  • View Data Sheet

    Description:

    SH2 domain containing 1A Human Recombinant

    SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP, SH2D1A, DSHP, SAP, LYP, XLP, EBVS, IMD5, XLPD, MTCP1, FLJ18687, FLJ92177, SAP/SH2D1A.

    Product # :

    PRO-006

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    Description

    SH2D1A Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 148 amino acids (1-128 a.a.) and having a molecular mass of 16.3kDa. The SH2D1A is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The SH2D1A solution (1 mg/ml) contains 20mM Tris-HCl Buffer (pH 7.5), 1mM DTT and 10% Glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      SH2D1A acts as an inhibitor of the signaling lymphocyte activation molecule (SLAM) self-association. The SH2D1A protein is expressed at a high level in the thymus and the lung, with a lower level of expression in the spleen and the liver. The SH2D1A protein contains an SH2 domain and a short tail. SH2D1A has a key role in the bidirectional stimulation of T and B cells. Defects in the SH2D1A gene cause the X-linked lymphoproliferative disease (XLPD), aka Duncan disease. The XLPD is distinguished by a rare congenital immunodeficiency following an EBV infection (Epstein-Barr virus).

    • Synonyms

      SH2 domain-containing protein 1A, Duncan disease SH2-protein, Signaling lymphocytic activation molecule-associated protein, SLAM-associated protein, T-cell signal transduction molecule SAP, SH2D1A, DSHP, SAP, LYP, XLP, EBVS, IMD5, XLPD, MTCP1, FLJ18687, FLJ92177, SAP/SH2D1A.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MDAVAVYHGK ISRETGEKLL LATGLDGSYL LRDSESVPGV YCLCVLYHGY IYTYRVSQTE TGSWSAETAP GVHKRYFRKI KNLISAFQKP DQGIVIPLQY PVEKKSSARS TQGTTGIRED PDVCLKAP.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sh2D1A Human

About SH2D1 / SH2 Domain Protein:

A protein usually found within the Src oncoprotein, Src Homology 2 is a conserved protein domain. While it’s primarily seen in the Src oncoprotein, it also turns up in some specific intracellular signal-transducing proteins. Finding it outside of these is rare, and they shouldn’t be found outside the adaptor proteins.

SH2D1 Binding
SH2 domains are only know to bind phosphorylated tyrosine residue, although this is typically if a target protein with a longer peptide motif. Out of the currently known pTyr-recognition domains, that makes SH2 the largest one to behave in such a way.

SH2 Domain Protein Diversity
The prominence of SH2 domains isn’t too diverse. While they don’t turn up in yeast and things of a similar makeup, they can be found in the boundary between animalia and protozoa. It can also be found in Dictyostelium discoideum.
Within the human body, studies have shown that there are 120 domains across 115 proteins in the human genome. That highlights a rapid rate of expansion across the domains.

SH2D1 Function
SH2 domains focus on recognizing tyrosine residues’ phosphorylated state. In doing so, it allows for the localization of these sites. There are multiple reactions involved in this process, such as sending a signal through membranes, which are then “sensed” by receptors. These will then result in other actions that focus on activating and localizing the site.
Once the process is completed, gene expressions and other responses should have altered patterns. In essence, the SH2 domain can be seen as a regulatory module that focuses on intracellular signalling cascades.
It typically interacts well with target peptides that contain high volumes of phosphotyrosine.