Serine Peptidase Inhibitor

Serine Peptidase Inhibitor

Serine Peptidase Inhibitor

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  • View Data Sheet

    Description:

    Serine Peptidase Inhibitor, Kunitz Type 2 Human Recombinant, Sf9

    Serine Peptidase Inhibitor, Kunitz Type, 2, Placental Bikunin, Hepatocyte Growth Factor Activator Inhibitor Type 2, Serine Protease Inhibitor, Kunitz Type, 2, HAI-2, HAI2, Kop, Kunitz-Type Protease Inhibitor 2, Placental, Bikunin, DIAR3, PB, Kunitz-type protease inhibitor 2.

    Product # :

    PRO-2219

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    Description

    SPINT2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 176 amino acids (28-197 a.a.) and having a molecular mass of 20kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SPINT2 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    SPINT2 protein solution (0.2mg/ml) contains phosphate buffered saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 80.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      SPINT2 is a transmembrane protein acts as an inhibitor of HGF activator. SPINT2 inhibits plasmin, plasma and tissue kallikrein, and factor XIa. SPINT2 has two extracellular Kunitz domains that inhibit few serine proteases. SPINT2 is assumed tumor suppressor, mutations in SPINT2 leads to a congenital sodium diarrhea.

    • Synonyms

      Serine Peptidase Inhibitor, Kunitz Type, 2, Placental Bikunin, Hepatocyte Growth Factor Activator Inhibitor Type 2, Serine Protease Inhibitor, Kunitz Type, 2, HAI-2, HAI2, Kop, Kunitz-Type Protease Inhibitor 2, Placental, Bikunin, DIAR3, PB, Kunitz-type protease inhibitor 2.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADRERSIHDF CLVSKVVGRC RASMPRWWYN VTDGSCQLFV YGGCDGNSNN YLTKEECLKK CATVTENATG DLATSRNAAD SSVPSAPRRQ DSEDHSSDMF NYEEYCTANA VTGPCRASFP RWYFDVERNS CNNFIYGGCR GNKNSYRSEE ACMLRCFRQQ ENPPLPLGSK HHHHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Spint2 Human Sf9
  • View Data Sheet

    Description:

    Serine Peptidase Inhibitor, Kunitz Type 2 Human Recombinant

    DIAR3, FLJ45571, HAI-2, HAI2, Kop, PB, Kunitz-type protease inhibitor 2, Hepatocyte growth factor activator inhibitor type 2, Placental bikunin, SPINT2.

    Product # :

    PRO-1296

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    Description

    SPINT2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids (28-197 a.a.) and having a molecular mass of 21.8kDa.SPINT2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    SPINT2 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      SPINT2 is a transmembrane protein acts as an inhibitor of HGF activator. SPINT2 inhibits plasmin, plasma and tissue kallikrein, and factor XIa. SPINT2 has two extracellular Kunitz domains that inhibit few serine proteases. SPINT2 is assumed tumor suppressor, mutations in SPINT2 leads to a congenital sodium diarrhea.

    • Synonyms

      DIAR3, FLJ45571, HAI-2, HAI2, Kop, PB, Kunitz-type protease inhibitor 2, Hepatocyte growth factor activator inhibitor type 2, Placental bikunin, SPINT2.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMADRER SIHDFCLVSK VVGRCRASMP RWWYNVTDGS CQLFVYGGCD GNSNNYLTKE ECLKKCATVT ENATGDLATS RNAADSSVPS APRRQDSEDH SSDMFNYEEY CTANAVTGPC RASFPRWYFD VERNSCNNFI YGGCRGNKNS YRSEEACMLR CFRQQENPPL PLGSK.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Spint2 Human
  • View Data Sheet

    Description:

    Serine Peptidase Inhibitor Kazal Type 7 Human Recombinant

    Serine peptidase inhibitor Kazal type 7, ECG2, ECRG2, Serine protease inhibitor Kazal-type 7, Esophagus cancer-related gene 2 protein, ECRG-2, SPINK7.

    Product # :

    PRO-1507

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    Description

    SPINK7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 89 amino acids (20-85) and having a molecular mass of 9.6 kDa.SPINK7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The SPINK7 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 2mM DTT.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Serine peptidase inhibitor Kazal type 7 (SPINK7) which includes one Kazal-like domain is most likely serine protease inhibitor. Diseases linked with SPINK7 include esophageal cancer, and oral squamous cell carcinoma

    • Synonyms

      Serine peptidase inhibitor Kazal type 7, ECG2, ECRG2, Serine protease inhibitor Kazal-type 7, Esophagus cancer-related gene 2 protein, ECRG-2, SPINK7.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSSEAASLS PKKVDCSIYK KYPVVAIPCP ITYLPVCGSD YITYGNECHL CTESLKSNGR VQFLHDGSC.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Spink7 Human

About SPIN / Serine Peptidase Inhibitor:

Serine peptidase inhibitor, also known as SPIN, encoded by the SPINT2 gene, and also called Kunitz-type protease inhibitor 2, is an enzyme inhibitor.

SPIN Function
SPIN is a transmembrane protein, meaning it spans the entirety of the cell membrane, acting as gateways to inhibit the movement of serine proteases. Serine proteases cleave peptide bonds in proteins. As such, SPIN may act as a tumor suppressor, preventing the passages of HGF activator which, in turn, prevents the formation of hepatocyte growth factor, which is secreted by mesenchymal cells and targets epithelial cells, endothelial cells, hemopoietic progenitor cells, and T cells.

Serine Peptidase Inhibitor Mechanism
SPIN mechanisms have been observed in suppressing OSCC cell migration and invasion, where it was seen that it contains gelatinolytic bands sensitive to GM6001, suggesting the presence of pro-matrix metalloprotease (MMP)-2 and -9. There is also evidence that the primary target protease for HAI-2 in the epithelial cell is prostasin.

SPIN Interactions
SPIN interacts with a range of other proteins, including AP5M1 AP-5 complex subunit mu-1 ), PRSS3 (Serine Protease 3), C19orf33 (Chromosome 19 Open Reading Frame 33), SLC26A3 (Solute Carrier Family 26 Member 3), EPCAM (Epithelial Cell Adhesion Molecule), MYO5B (Myosin VB), PRSS8 (Serine Protease 8), ST14 (ST14 Transmembrane Serine Protease Matriptase), and MST1 (Macrophage Stimulating 1) .

Serine Peptidase Inhibitor Structure
SPIN has a size of 252 amino acids and a molecular mass of 28228 Da.