Selenoprotein

Selenoprotein

Selenoprotein

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  • View Data Sheet

    Description:

    Selenoprotein X 1 Human Recombinant

    Methionine-R-sulfoxide reductase B1, MsrB1, Selenoprotein X, SelX, SEPX1, SELR, SELX, HSPC270, MGC3344.

    Product # :

    PRO-260

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    Description

    SEPX1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 136 amino acids (1-116 a.a.) and having a molecular mass of 14.8kDa. In bacteria, the selenocystein (Sec/U) element is positioned directly following the UGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. The SEPX1 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The SEPX1 solution (0.5 mg/ml) contains 20mM Tris-HCl Buffer (pH 7.5), 1mM DTT, 0.1mM PMSF, 2mM EDTA and 10% Glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Methionine sulfoxide reductase B1 (SEPX1 or MSRB1), is a selenoprotein that contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that usually signals translation termination. SEPX1 is a member of the methionine sulfoxide reductase B (MsrB) family, and is expressed in an assortment of adult and fetal tissues. MSRs (Methionine sulfoxide reductases) catalyze the reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS creates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two separate enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO.

    • Synonyms

      Methionine-R-sulfoxide reductase B1, MsrB1, Selenoprotein X, SelX, SEPX1, SELR, SELX, HSPC270, MGC3344.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sepx1 Human
  • View Data Sheet

    Description:

    Selenoprotein W 1 Human Recombinant

    Selenoprotein W, 1, SelW, Selenoprotein W.

    Product # :

    PRO-2082

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    Description

    SEPW1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids (1-87 a.a) and having a molecular mass of 11.8kDa. SEPW1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    SEPW1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Selenoprotein W 1, also known as SEPW1 is a selenoprotein, which has a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded through the UGA codon which normally signals translation termination. The 3' UTR of selenoprotein genes share a common stem-loop structure, the sec insertion sequence (SECIS), which is essential for the recognition of UGA as a Sec codon instead of as a stop signal. SEPW1 shows highest expression in skeletal muscle and heart, and also involved in oxidation-reduction reactions.

    • Synonyms

      Selenoprotein W, 1, SelW, Selenoprotein W.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sepw1 Human
  • View Data Sheet

    Description:

    15 KDa Selenoprotein Human Recombinant

    15 KDa Selenoprotein, SEP15.

    Product # :

    PRO-1468

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    Description

    SEP15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 160 amino acids (29-165 a.a) and having a molecular mass of 17.7kDa.SEP15 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    SEP15 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      15 KDa Selenoprotein (SEP15) is a protein-coding gene which contains a selenocysteine (Sec) residue at its active site. Theselenocysteine is encoded by the UGA codon that usually signals translation termination. The 3' UTR ofselenoprotein genes have a conventional stem-loop structure, the sec insertion sequence (SECIS), which isnecessary for the recognition of UGA as a Sec codon rather than as a stop signal. Studies in mouse propose thatthis selenoprotein may have redox function and may be implicated in the quality control of protein folding. This geneis localized on chromosome 1p31, a genetic locus usually mutated or deleted in human cancers.Diseases associated with SEP15 include lung cancer susceptibility, and chronic lymphocytic leukemia.

    • Synonyms

      15 KDa Selenoprotein, SEP15.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSVSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Sep15 Human

About SEP / Selenoprotein:

There are three principal families of selenoprotein, and they each have different identified functions.
●Glutathione Peroxidases: There are four known enzymes in this family. These enzymes all catabolize hydroperoxide, removing glutathione. This means that they have antioxidant functions.
●Iodothyronine Deiodinases: There are three known enzymes in this family. They are known to have antioxidant properties, and they regulate cellular protein thiol redox status by reducing thioredoxin.
●Thioredoxin Reductases: There are three known enzymes in this family. Collectively they regulate thyroid hormone and are thought to be important in metabolic regulation and embryonic development.
Among other types of selenoprotein are:
●Selenoprotein W, which has antioxidant properties.
●Methionine sulfoxide reductase B, which may remove an oxygen species.
●Selenoprotein P, which is thought to play a role in selenium transport and be an antioxidant.

SEP Function
Together and separately, selenoproteins are hypothesised to play a role in neutralization of free radicals and potentially the slowing of the aging process ; inflammation and immunity responses ; preventing and repairing damage from muscular dystrophy, cardiovascular disease, neurological disease (such as Alzheimer’s and Parkinson’s), and cancer . They may play an important role in the endocrine system regulating thyroid hormone and in male fertility through spermatogenisis . They are also understood to have important roles in cell maintenance, skeletal muscle regeneration, and calcium homeostasis.
On a normal diet, the highest selenium levels are found in the kidney and liver, with decreasing levels in the spleen, pancreas, heart, and brain. But when dietary selenium is scarce, the brain retains its selenium longest. This may indicate that selenium, and thus the selenoproteins, play a significant role in the central nervous system