Regulator of Calcineurin

Regulator of Calcineurin

Regulator of Calcineurin

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  • View Data Sheet

    Description:

    Regulator of Calcineurin 3 Human Recombinant

    DSCR1L2, hRCN3, MCIP3, RCN3, Calcipressin-3, Down syndrome candidate region 1-like protein 2, Myocyte-enriched calcineurin-interacting protein 3, Regulator of calcineurin 3.

    Product # :

    PRO-1284

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    Description

    RCAN3 Human Recombinant produced in E. coli is a single polypeptide chain containing 209 amino acids (56-241) and having a molecular mass of 23.5 kDa. RCAN3 is fused to 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The RCAN3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 20% glycerol and 0.2M NaCl.

    Purity

    Greater than 90% as determined by SDS-PAGE.

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    • Introduction

      Calcipressin-3 (RCAN3) takes part in central nervous system development. RCAN3 inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Overexpression of calcipressin-3 results in inhibition of calcineurin activity towards the nuclear factor of activated T-cells (NFAT) transcription factors and also downregulates NFAT-dependent cytokine gene expression in activated Jurkat T-cells. Highest expression takes place s in heart, skeletal muscle kidney, liver and peripheral blood leukocytes.

    • Synonyms

      DSCR1L2, hRCN3, MCIP3, RCN3, Calcipressin-3, Down syndrome candidate region 1-like protein 2, Myocyte-enriched calcineurin-interacting protein 3, Regulator of calcineurin 3.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSEAVFEAR EQKERFEALF TIYDDQVTFQ LFKSFRRVRI NFSKPEAAAR ARIELHETDF NGQKLKLYFA QVQMSGEVRD KSYLLPPQPV KQFLISPPAS PPVGWKQSED AMPVINYDLL CAVSKLGPGE KYELHAGTES TPSVVVHVCE SETEEEEETK NPKQKIAQTR RPDPPTAALN EPQTFDCAL.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rcan3 Human
  • View Data Sheet

    Description:

    Regulator of Calcineurin 2 Human Recombinant

    Regulator of calcineurin 2, DSCR1L1, MCIP2, Down syndrome critical region gene 1-like 1, ZAKI4, Myocyte-enriched calcineurin-interacting protein 2, Thyroid hormone-responsive protein ZAKI-4, CSP2, RCN2, thyroid hormone-responsive (skin fibroblasts).

    Product # :

    PRO-990

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    Description

    RCAN2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 217 amino acids (1-197 a.a.) and having a molecular mass of 24.1kDa.RCAN2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    RCAN2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 1mM DTT and 20% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      RCAN2 is a member of the RCAN (Regulator of calcineurin) family that controls the activity of Calcineurin using a valine-rich region placed in their carboxyl tail. RCANs race against NFAT (nuclear factor of activated T-cells) to bind to the same docking site in calcineurin, and consequently inhibits or activates calcineurin activity. RCAN2 is a Ca(2+) dependent protein phosphatase which takes part in numerous neuornal functions.

    • Synonyms

      Regulator of calcineurin 2, DSCR1L1, MCIP2, Down syndrome critical region gene 1-like 1, ZAKI4, Myocyte-enriched calcineurin-interacting protein 2, Thyroid hormone-responsive protein ZAKI-4, CSP2, RCN2, thyroid hormone-responsive (skin fibroblasts).

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MPAPSMDCDV STLVACVVDV EVFTNQEVKE KFEGLFRTYD DCVTFQLFKS FRRVRINFSN PKSAARARIE LHETQFRGKK LKLYFAQVQT PETDGDKLHL APPQPAKQFL ISPPSSPPVG WQPINDATPV LNYDLLYAVA KLGPGEKYEL HAGTESTPSV VVHVCDSDIE EEEDPKTSPK PKIIQTRRPG LPPSVSN

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rcan2 Human
  • View Data Sheet

    Description:

    Regulator of Calcineurin 1 Human Recombinant

    Calcipressin-1, Regulator of calcineurin 1, Down syndrome critical region protein 1, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Adapt78, RCAN1, ADAPT78, CSP1, DSC1, DSCR1, RCN1.

    Product # :

    PRO-319

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    • description
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    Description

    RCAN1 Isoform-b Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 117 amino acids and having a molecular mass of 13 kDa.

    Source

    Escherichia Coli.

    Formulation

    The protein solution (1mg/ml) contains 20mM Tris-HCl pH-7.5, 100mM NaCl and 1mM DTT.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      RCAN1 encoded by a gene located in the human chromosome 21, interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. RCAN1 is located in the minimal candidate region for the Down syndrome phenotype, and is over expressed in the brain of Down syndrome fetuses. Chronic over expression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene.
      DSCR1 Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A, possibly affecting central nervous system development.

    • Synonyms

      Calcipressin-1, Regulator of calcineurin 1, Down syndrome critical region protein 1, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Adapt78, RCAN1, ADAPT78, CSP1, DSC1, DSCR1, RCN1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MKLYFAQTLH IGSSHLAPPN PDKQFLISPP ASPPVGWKQV EDATPVINYD LLYAISKLGPGEKYELHAAT DTTPSVVVHV CESDQEKEEE EEMERMRRPK PKIIQTRRPE YTPIHLS.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rcan1 Human

About RCAN / Regulator of Calcineurin:

RCAN1 is a protein that's involved in some complicated processes. It's related to degenerative nerve disease, inflammation, and protein glycosylation (meaning that it attaches sugar molecules to proteins). Down syndrome and Alzheimer's disease are two diseases where RCAN1 is thought to play an important role.

RCAN Interaction
Researchers have recently looked at the interaction between calcineurin and other substrates, such as targeting proteins and regulators of calcineurin activity. They found that RCAN binds to calcineurin, and when it does, the dephosphorylation of proteins by calcineurin is inhibited. When RCANs bind to Calcines, they inhibit their function, which then prevents the activation or suppression of gene transcription by

Regulator of Calcineurin Function
The Regulator of Calcineurin performs an important function in the body by regulating calcium homeostasis. It helps to maintain calcium ion concentration and prevents hypercalcemia by dephosphorylating calcineurin. Calcineurin is an enzyme found in mammals that regulates calcium homeostasis by dephosphorylating proteins. It functions as a phosphatase and plays a critical role in controlling the concentration of calcium ions and prevents hypercalcemia.

RCAN Structure
The structure of the Regulator of Calcineurin is not yet known; however, it is thought that it forms a pentameric protein complex. The calcineurin structure, also known as the calcium-dependent phosphohydrolase, has two subunits: subunit A contains a Fe2+ ion and a Zn2+ ion and is the catalytic site of the enzyme's function, while subunit B is involved in its regulatory mechanism by binding calcium-binding protein calmodulin.

Regulator of Calcineurin Mechanism
There are innate mechanisms that limit how far you can go with neural sequence transplants. But we don't know them very well. It shows that RCAN1, a feedback inhibitor of calcineurin, suppresses two distinct mechanisms of non-shivering thermogenesis.