RAN Binding Protein

RAN Binding Protein

RAN Binding Protein

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  • View Data Sheet

    Description:

    RAN Binding Protein 3 Human Recombinant

    RAN binding protein 3, RanBP3.

    Product # :

    PRO-1986

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    Description

    RANBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (235-445 a.a) and having a molecular mass of 25.5kDa. RANBP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    RANBP3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      RAN Binding Protein 3, also known as RANBP3, is found in the nucleus and cytoplasm. RANBP3 which owns RanBD1 domain participates in nuclear export as part of a heteromeric complex. RANBP3 is also negatively regulates TGF-beta signaling by interacting with the R-SMAD proteins, SMAD2 and SMAD3.

    • Synonyms

      RAN binding protein 3, RanBP3.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSISSSLEN STNSADASSN KFVFGQNMSE RVLSPPKLNE VSSDANRENA AAESGSESSS QEATPEKESL AESAAAYTKA TARKCLLEKV EVITGEEAES NVLQMQCKLF VFDKTSQSWV ERGRGLLRLN DMASTDDGTL QSRLVMRTQG SLRLILNTKL WAQMQIDKAS EKSIRITAMD TEDQGVKVFL ISASSKDTGQ LYAALHHRIL ALRS.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Ranbp3 Human
  • View Data Sheet

    Description:

    RAN Binding Protein 1 Human Recombinant

    RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1.

    Product # :

    PRO-1064

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    Quantity :

    Shipping Method :

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    • description
    • source
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    • More Info

    Description

    RANBP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 225 amino acids (1-201) and having a molecular mass of 25.8kDa (molecular size on SDS-PAGE will appear higher).RANBP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The RANBP1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      RANBP1 is a member of a family of proteins which bind Ran GTPase. Ran GTPase is vital for cell cycle control, mRNA processing, nuclear transport, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 does not stimulate GTPase activity of RAN however RANBP1 does distinctly raise GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). Additionally, RANBP1 operates as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

    • Synonyms

      RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Ranbp1 Human

About RANBP / RAN Binding Protein:

The RAN binding protein is an enzyme. It is present in humans, where it is encoded by the RANBP1 gene. The RANBP will interact exclusively with GTP-charged Ran. It encodes the 23-kD protein, which binds to RAn complexed -- not with GDP, but with GTP. It will not activate the GTPase activity of RAN. However, it will have a big impact on GTP hydrolysis by the RANGTPase-activating protein. Furthermore, the RANBP1 cDNA will encode a 201-amino acid protein if it is 92% similar to its mouse homolog. The RANBP1 will act as a negative regulator of RCC1 by inhibiting RCC!-stimulate guanine nucleotide release from RAN. It will bind to two different types of effector that you’ll find within the cell. The RAN-binding proteins play a role in ending export processes from the nucleus to the cytoplasm. Researchers have also found that they have importin-beta-like molecules -- these help to bind cargo proteins during nuclear transport.

RANBP Structure
You’ll find the ran-binding domain, which is a conserved sequence motif, in several proteins that are involved in the transport processes. RanBP2 contains four domains, which means it is responsible for a significant portion of the cytoplasmic fibrils, which come from the nuclear-pore complex. In terms of structure, the ran bound to a non-hydrolysable GTP analogue in complex with the first ran-binding domain -- in the human RanBP2 -- shows that the RanBD1 has a pleckstrin-homology domain fold. It has also been shown that the switch-I region of Ran x GppNHp is similar to the canonical Ras GppNHp structure.