RAD51

RAD51

RAD51

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  • View Data Sheet

    Description:

    RAD51D Human Recombinant

    BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.

    Product # :

    PRO-1832

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    Description

    RAD51D Human Recombinant produced in E. coli is. a single polypeptide chain containing 239 amino acids (1-216 a.a) and having a molecular mass of 25.7kDa. RAD51D is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    RAD51D solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

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    • Introduction

      RAD51D is a member of the RAD51 protein family whose members are extremely similar to bacterial RecA and Saccharomyces cerevisiae Rad51. RAD51 protein family members take part in the homologous recombination and repair of DNA. RAD51D forms a complex with several other members of the RAD51 family which catalyzes homologous pairing between single- and double-stranded DNA, and participates in the early stage of recombinational repair of DNA.

    • Synonyms

      BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KAEALRRIQV VHAFDIFQML DVLQELRGTV AQQVTGSSGT VKVVVVDSVT AVVSPLLGGQ QREGLALMMQ LARELKTLAR DLGMAVVVTN HITRDRDSGR LKPALGRSWS FVPSTRILLD TIEGAGASGG RRMACLAKSS RQPTGFQEMV DIGTWGTSEQ SATLQGDQT.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rad51D Human
  • View Data Sheet

    Description:

    RAD51D (1-328 a.a.) Human Recombinant

    BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.

    Product # :

    PRO-1861

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    Description

    RAD51D (1-328) Human Recombinant produced in E. coli is. a single polypeptide chain containing 351 amino acids and having a molecular mass of 37.4kDa. RAD51D (1-328) is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    RAD51D (1-328) solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      RAD51D is a member of the RAD51 protein family whose members are extremely similar to bacterial RecA and Saccharomyces cerevisiae Rad51. RAD51 protein family members take part in the homologous recombination and repair of DNA. RAD51D forms a complex with several other members of the RAD51 family which catalyzes homologous pairing between single- and double-stranded DNA, and participates in the early stage of recombinational repair of DNA.

    • Synonyms

      BROVCA4, R51H3, RAD51L3, TRAD, DNA repair protein RAD51 homolog 4, RAD51 homolog D, RAD51-like protein 3, RAD51D.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMGVLRVG LCPGLTEEMI QLLRSHRIKT VVDLVSADLE EVAQKCGLSY KALVALRRVL LAQFSAFPVN GADLYEELKT STAILSTGIG SLDKLLDAGL YTGEVTEIVG GPGSGKTQVC LCMAANVAHG LQQNVLYVDS NGGLTASRLL QLLQAKTQDE EEQAEALRRI QVVHAFDIFQ MLDVLQELRG TVAQQVTGSS GTVKVVVVDS VTAVVSPLLG GQQREGLALM MQLARELKTL ARDLGMAVVV TNHITRDRDS GRLKPALGRS WSFVPSTRIL LDTIEGAGAS GGRRMACLAK SSRQPTGFQE MVDIGTWGTS EQSATLQGDQ T.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rad51D 1 328 Human
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    Description:

    RAD51B Human Recombinant

    RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, Rad51B, RAD51-like protein 1, RAD51B.

    Product # :

    PRO-1804

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    Description

    RAD51B Human Recombinant produced in E. coli is. a single polypeptide chain containing 373 amino acids (1-350) and having a molecular mass of 40.6kDa. RAD51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The RAD51B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      RAD51 family members are evolutionarily conserved proteins vital for DNA repair by homologous recombination. RAD51B is a part of the RAD51 protein family. RAD51B is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks occurring during DNA replication or induced by DNA-damaging agents. RAD51B forms a stable heterodimer with the family member RAD51C, which interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of RAD51B leads to cell cycle G1 delay and cell apoptosis.

    • Synonyms

      RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, Rad51B, RAD51-like protein 1, RAD51B.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Rad51B Human

About RAD51:

RAD51 is a gene that controls a cell's ability to repair damage to DNA. It does this by binding to single-stranded DNA and helping it form a double helix, which is the necessary first step in the process of replication. RAD51 is part of an important family of genes called recombinases, which allow for precise copying and pasting of genetic material.
The best-known recombinase is probably encoded by the chicken (or avian) papillomavirus (CPV). This virus inserts its genome into that of its host cell and needs RAD51's ability to copy itself before infecting new cells. It is often shared with TRAD, which has the potential to aid with DNA binding. It has been found in all known living organisms, from bacteria to humans.

RAD51 Function
The RAD51 gene's protein product is a key component of DNA repair, which is how our cells maintain their stability and prevent mutations from accumulating. In the event that the cell does sustain damage to its DNA, such as occurs from ultraviolet radiation or certain chemicals, this protein then aids in repairing the damage.

RAD51 Interaction
RAD51 directly interacts with XRCC3, which is a protein that helps RAD51 bind more strongly to the broken strands of DNA; XRCC5, which is another protein RAD51 relies on for strand alignment; CDK6, which prevents the cell from committing suicide when it detects a chromosome break; and PARP1, which repairs damage caused by radiation exposure.

RAD51 Structure
RAD51 consists of two alpha-helices and three beta-strands. This is an important structure as it assists in DNA repair. It does this by binding to both single-stranded and double-stranded DNA molecules and then interacting with other proteins to form a helicase which unwinds the DNA for repair.

RAD51 Mechanism
While its mechanism isn't fully understood, it is thought that to deal with breaks and other errors in our DNA, since we have cells that are constantly searching for a way to fix them. When they find one, they move on it quickly, like a band-aid pulled off quickly or a zipper unzipped quickly. There are two different types of errors that can be repaired: insertion/deletion loops and nucleotide mismatches - which can be thought of as broken.