Nucleopurin

Nucleopurin

Nucleopurin

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  • View Data Sheet

    Description:

    Nucleopurin 62kDa C-Terminal Like Human Recombinant

    FLJ20130, RP13-383K5.2, Nucleoporin-62 C-terminal-like protein, NUP62L, NUP62CL, Nucleopurin 62kDa C-Terminal Like.

    Product # :

    PRO-1906

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    Description

    NUP62CL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 95 amino acids (1-72 a.a) and having a molecular mass of 10.3kDa.NUP62CL is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    NUP62CL protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

    Purity

    Greater than 80.0% as determined by SDS-PAGE.

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    • Introduction

      Nucleopurin 62kDa C-Terminal Like, which is also known as NUP62CL, is a protein-coding gene. NUP62CL contains a domain found in nucleoporins which are glycoproteins located in nuclear pore complexes.

    • Synonyms

      FLJ20130, RP13-383K5.2, Nucleoporin-62 C-terminal-like protein, NUP62L, NUP62CL, Nucleopurin 62kDa C-Terminal Like.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMQFTSIS NSLTSTAAIG LSFTTSTTTT ATFTTNTTTT ITSGFTVNQN QLLSRGFENL VPYTSTVRFV FYMEK.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nup62Cl Human
  • View Data Sheet

    Description:

    Nucleopurin 62kDa Human Recombinant

    Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62, p62, IBSN, SNDI.

    Product # :

    PRO-999

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    Description

    NUP62 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66 kDa. NUP62 is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9 insect cells.

    Formulation

    NUP62 is supplied in 20mM HEPES buffer pH-8, 200mM NaCl and 20% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      Nucleopurin 62kDa (NUP62) belongs to the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. Nucleoporins are the principal components of the nuclear pore complex in eukaryotic cells. This complex is a colossal structure which extends across the nuclear envelope, forming an entryway that regulates the stream of macromolecules between the nucleus and the cytoplasm. NUP62 associates with the importin alpha/beta complex that is involved in the import of proteins containing nuclear localization signals. Defects in the NUP62 are the cause of SNDI (infantile striatonigral degeneration), aka infantile bilateral striatal necrosis (IBSN) or familial striatal degeneration.

    • Synonyms

      Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62, p62, IBSN, SNDI.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

    • Immunological Functions

      1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera), immunodot analysis with positive/negative samples.

    • coating concentration

      0.5-1.0 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

    • Applications

      Western blot with Polyclonal anti-Nup62 antibodies.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nup62 Human
  • View Data Sheet

    Description:

    Nucleopurin 210kDa Recombinant Human

    Nucleoporin 210kDa, KIAA0906, GP210, Nuclear envelope pore membrane protein POM 210, Nuclear pore protein gp210, nuclear pore membrane glycoprotein 210.

    Product # :

    PRO-109

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    Description

    Recombinant NUP210 protein is a construct carrying multiple concatenated copies of the short cytoplasmic gp210 C-terminus (which contains the autoreactive epitopes) and having a molecular mass of 27kDa (pH 9.8). NUP210 protein is fused to a hexa-histidine purification tag.

    Source

    Sf9 insect cells.

    Formulation

    NUP210 is supplied in 20mM HEPES buffer pH-7.9 and 6M Urea.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      The NUP210 complex is a immense structure which spreads through the nuclear envelope, forming a doorway which regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the core components of the nuclear pore complex in eukaryotic cells. NUP210 is a membrane-spanning glycoprotein and is a major component of the nuclear pore complex.

    • Synonyms

      Nucleoporin 210kDa, KIAA0906, GP210, Nuclear envelope pore membrane protein POM 210, Nuclear pore protein gp210, nuclear pore membrane glycoprotein 210.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

    • Immunological Functions

      1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test.

    • coating concentration

      0.4-1.0 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

    • Applications

      Western blot with PBC sera or monoclonal anti-hexa-His-tag antibody.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nup210 Human

About NUP / Nucleoporin:

NUP Mechanism and Interactions
Nucleoporins (NUP) are the regulators when it comes to the moving of macromolecules through the nuclear envelope through interaction with karyopherins molecules. Karyopherins will bind to their contents. It will also mingle with the FG-repeats that some nucleoporins contain. Until they can diffuse through nuclear pores, Karyopherins and their contents repeatedly move between the FG-repeats and then through the nuclear pore complex. Karyopherins can either transport protein into the nucleus out of the nucleus. Then, driven by the G protein (Ran), which is so tiny that it can diffuse through nuclear pores down, Karyopherins release their contents.

Nucleoporin Function
Nucleoporins (NUP) are fragments of the nuclear pore complex. It regulates the movement of macromolecules across the nuclear envelope. Due to their molecular weights, they got their names from. The protein RANBD (RAN), NTD (N-terminal domain), and CTD (C-terminal domain) is bound to a domain contained in them.
●For the Nuclear protein import, maintenance of nuclear architecture, and mRNA export, NUP1P (a yeast nucleoprotein) is required.
●Bidirectional transport of importin a requires NUP2P (a yeast nucleoprotein)
●mRNA export requires NUP Gle1 (a human and yeast nucleoprotein)

NUP Structure
NUP is the cumulative name for the 30 different nuclear proteins which are included in the eightfold symmetrical structure of the nuclear pore complexes (NPC). The NPS is an eight-sided ring that crosses the nuclear envelope. Another eight scaffold sub-complexes consist of the eight-sided ring. Some proteins that line the pore are interlayered with two structural layers of COPII-like coating. There are three layers (cytoplasm, nucleoplasm, and inner pore) of the ring complex, which can be found from the cytoplasm to the nucleoplasm.