NHP2

NHP2

NHP2

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  • View Data Sheet

    Description:

    NHP2 non-histone chromosome protein 2-like Human Recombinant

    FA-1, SPAG12, SNRNP15-5, SNU13, 15.5K, U4/U6.U5 tri-snRNP 15.5 kDa protein, NHP2 Non-Histone Chromosome protein 2-like 1 (S. cerevisiae), Sperm Specific Antigen 1, SSFA1, NHPX, FA1, OTK27, High Mobility Group-like Nuclear Protein 2 homolog 1.

    Product # :

    PRO-038

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    • description
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    • formulation
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    Description

    NHP2L1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-128a.a.) and having a molecular mass of 16.7kDa.NHP2L1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The NHP2L1 protein solution (0.25mg/1ml) is formulated in In 20 mM Tris-HCl Buffer (pH 7.5), 100 mM NaCl, and 10% Glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      NHP2L1 is a member of the ribosomal protein L7Ae family. NHP2L1 protein limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 directly binds to the 5' stem-loop of U4 snRNA and has a vital part in the late stage of spliceosome assembly, prior to step I of splicing catalysis.

    • Synonyms

      FA-1, SPAG12, SNRNP15-5, SNU13, 15.5K, U4/U6.U5 tri-snRNP 15.5 kDa protein, NHP2 Non-Histone Chromosome protein 2-like 1 (S. cerevisiae), Sperm Specific Antigen 1, SSFA1, NHPX, FA1, OTK27, High Mobility Group-like Nuclear Protein 2 homolog 1.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nhp2L1 Human
  • View Data Sheet

    Description:

    NHP2 Ribonucleoprotein Homolog Human Recombinant

    H/ACA ribonucleoprotein complex subunit 2, Nucleolar protein family A member 2, snoRNP protein NHP2, NHP2, NOLA2, NHP2P.

    Product # :

    PRO-067

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    • description
    • source
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    • purity
    • More Info

    Description

    NHP2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 173 amino acids (1-153 a.a.) and having a molecular mass of 19.3kDa. The NHP2 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The NHP2 solution (0.25 mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      NHP2 belongs to the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been organized into 2 families: C/D and H/ACA. NHP2 (NOLA2) forms a small ribonucleoprotein particle with GAR1 (NOLA1), Nop10 for the isomerization of uridine to pseudouridine. NHP2 is involved in a variety of aspects of rRNA processing and modification. NHP2 localizes to the dense fibrillar component of the nucleolus and in nuclear Cajal bodies.

    • Synonyms

      H/ACA ribonucleoprotein complex subunit 2, Nucleolar protein family A member 2, snoRNP protein NHP2, NHP2, NOLA2, NHP2P.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MTKIKADPDG PEAQAEACSG ERTYQELLVN QNPIAQPLAS RRLTRKLYKC IKKAVKQKQI RRGVKEVQKF VNKGEKGIMV LAGDTLPIEV YCHLPVMCED RNLPYVYIPS KTDLGAAAGS KRPTCVIMVK PHEEYQEAYD ECLEEVQSLP LPL.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nhp2 Human

About NHP2:

The function of NHP2 (NHP2 ribonucleoprotein) is to be a non-catalytic component of the H/ACA small nucleolar ribonucleoprotein. It is essential for ribosome biogenesis and catalyses pseudouridylation of rRNA. Part of this is the isomerization of uridine.


NHP2 Gene
NHP2 is a gene that codes for protein. Dyskeratosis congenita, autosomal recessive 2, and autosomal recessive 1 are diseases linked to NHP2. Telomere C-strand (lagging strand) synthesis and ribosome biogenesis are two related processes in eukaryotes. Annotations in the gene ontology (GO) for this gene include snoRNA binding. RPL7A is a significant paralog of this gene.

NHP2 Function
NHP2 is essential for ribosome synthesis and the preservation of telomeres. It is a component of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex that catalyzes rRNA pseudouridylation. This entails isomerizing uridine such that the ribose is linked to C5 rather than the usual N1. Each rRNA can have up to 100 pseudouridine ('psi') residues, which may help to maintain the rRNA's shape. TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme, may also need proper processing or intranuclear trafficking.
H/ACA ribonucleoprotein complex subunit 2 is a protein that is encoded by the NHP2 gene in humans. The H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family includes this gene. SnoRNPs are engaged in several areas of rRNA processing and modification and are divided into two families: C/D and H/ACA.