MOB1 Mps One Binder Kinase Activator

MOB1 Mps One Binder Kinase Activator

MOB1 Mps One Binder Kinase Activator

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  • Name :

    MOBKL1B Human

    Description:

    MOB1, Mps One Binder Kinase Activator-Like 1B Human Recombinant

    Mps one binder kinase activator-like 1B, Mob1 alpha, Mob1A, Mob1 homolog 1B, Protein Mob4B, MOBKL1B, C2orf6, MOB4B, MOBK1B, MOB1, MATS1, FLJ10788, FLJ11595.

    Product # :

    PRO-068

    Price :

    Quantity :

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    • description
    • source
    • formulation
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    Description

    MOBKL1B Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 236 amino acids (1-216 a.a.) and having a molecular mass of 27.2kDa. The MOBKL1B is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The MOBKL1B solution (0.5mg/ml) 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT and 30% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      MOBKL1B is a member of the MOB1/phocein family. MOBKL1B stimulates the kinase activity of STK38 and STK38L. MOBKL1B also binds to and regulates downstream targets such as the NDR-family protein kinases and LATS1 kinase. Thus, MOBKL1B participates in cell cycle checkpoint control and tumor inhibition.

    • Synonyms

      Mps one binder kinase activator-like 1B, Mob1 alpha, Mob1A, Mob1 homolog 1B, Protein Mob4B, MOBKL1B, C2orf6, MOB4B, MOBK1B, MOB1, MATS1, FLJ10788, FLJ11595.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MSFLFSSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRQAVMLPEG EDLNEWIAVN TVDFFNQINM LYGTITEFCT EASCPVMSAG PRYEYHWADG TNIKKPIKCS APKYIDYLMT WVQDQLDDET LFPSKIGVPF PKNFMSVAKT ILKRLFRVYA HIYHQHFDSV MQLQEEAHLN TSFKHFIFFV QEFNLIDRRE LAPLQELIEK LGSKDR.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Mobkl1B Human

  • Name :

    MOBKL3 Human

    Description:

    MOB1, Mps One Binder kinase Activator-Like 3 Human Recombinant

    Mps one binder kinase activator-like 3, 2C4D, Class II mMOB1, Mob1 homolog 3, Mob3, Preimplantation protein 3, MOBKL3, MOB3, PREI3, MOB1, PREI3, CGI-95, MGC12264.

    Product # :

    PRO-049

    Price :

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    Shipping Method :

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    • description
    • source
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    • More Info

    Description

    MOBKL3 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 245 amino acids (1-225 a.a.) and having a molecular mass of 28.1kDa. The MOBKL3 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The MOBKL3 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0) containing 0.2M Nacl, 5mM DTT, 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Mps one binder kinase activator-like 3 (MOBKL3) is a member of the MOB1/Phocein family and is phosphorylated on serine residues. MOBKL3 is usually linked with membranes but can be present in the cytosol, where it behaves as a protein complex. MOBKL3 is the major partner of the striatin family members, which are scaffolding proteins involved in signaling and trafficking.

    • Synonyms

      Mps one binder kinase activator-like 3, 2C4D, Class II mMOB1, Mob1 homolog 3, Mob3, Preimplantation protein 3, MOBKL3, MOB3, PREI3, MOB1, PREI3, CGI-95, MGC12264.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD CSNIDKILEP PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM TATEQWIFLC AAHKTPKECP AIDYTRHTLD GAACLLNSNK YFPSRVSIKE SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE YENETFLCHR FTKFVMKYNL MSKDNLIVPI LEEEVQNSVS GESEA.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Mobkl3 Human

About MOBKL / MOB1, Mps One Binder Kinase Activator:

MOB1 is a multifunctional protein best known for its integrative role in regulating metazoan Hippo and NDR pathway signaling and yeast Mitotic Exit Network signaling. Human MOB1 binds the upstream kinases MST1 and MST2 and the downstream AGC group kinases LATS1, LATS2, NDR1, and NDR2. MOB1 binds to MST1 and MST2 via its phosphopeptide-binding infrastructure, the specificity of which matches MST1 and MST2 phosphorylation consensus.

MOBKL Mechanism
MOB1 binding to the LATS and NDR kinases, on the other hand, is mediated by a distinct interaction surface on MOB1. MOB1 facilitates the activation of downstream kinases by upstream kinases via a trans-phosphorylation event by assembling both upstream and downstream kinases into a single complex. MOB1 binding to its upstream partners also makes MOB1 a substrate, which allows it to regulate its two protein interaction activities differentially (at least in vitro). In addition to associating with MST1 (and MST2), previous interaction proteomics analysis revealed that MOB1A and MOB1B could associate in a phosphorylation-dependent manner with at least two other signaling complexes, one containing the Rho guanine exchange factors (DOCK6-8) and the other containing the serine/threonine phosphatase PP6.

MOB1, Mps One Binder Kinase Activator Structure
The MPS One Binder (MOB) 1 protein is an evolutionarily conserved eukaryotic protein family (with sizes ranging from 210–314 amino acids), the founding member discovered in yeast via interaction screens the kinase Mps1 and shown to be critical for mitotic exit. S. cerevisiae contains two yeast MOB proteins, Mob1 and Mob2. They act as AGC group kinase regulators, with Mob1 facilitating Dbf2 kinase activation in the Mitotic Exit Network pathway and Mob2 facilitating Cbk1 kinase activation in the RAM (regulation of Ace2 and morphogenesis) network pathway.