MAD2

MAD2

MAD2

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  • View Data Sheet

    Description:

    MAD2L1 Binding Protein Human Recombinant

    MAD2L1-binding protein, Caught by MAD2 protein, MAD2L1BP, CMT2, KIAA0110, RP1-261G23.6.

    Product # :

    PRO-1052

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    Description

    MAD2L1BP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-274 a.a) and having a molecular mass of 33.6kDa (Molecular weight on SDS-PAGE will appear higher).MAD2L1BP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    MAD2L1BP protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 100mM NaCl.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      MAD2L1-binding protein (MAD2L1BP) is a member of the MAD2L1BP family.MAD2L1BP is a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a fundamental factor of the spindle checkpoint which delays the onset of anaphase until all the kinetochores are connected to the spindle. The MAD2L1BP protein acts to curb the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 once it dissociated from the MAD2L1-CDC20 complex. For the duration of the cell cycle, MAD2L1BP levels increase and then remain constant until late mitosis after which they drop.

    • Synonyms

      MAD2L1-binding protein, Caught by MAD2 protein, MAD2L1BP, CMT2, KIAA0110, RP1-261G23.6.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMAAPEA EVLSSAAVPD LEWYEKSEET HASQIELLET SSTQEPLNAS EAFCPRDCMV PVVFPGPVSQ EGCCQFTCEL LKHIMYQRQQ LPLPYEQLKH FYRKPSPQAE EMLKKKPRAT TEVSSRKCQQ ALAELESVLS HLEDFFARTL VPRVLILLGG
      NALSPKEFYE LDLSLLAPYS VDQSLSTAAC LRRLFRAIFM ADAFSELQAP PLMGTVVMAQ GHRNCGEDWF RPKLNYRVPS RGHKLTVTLS CGRPSIRTTA WEDYIWFQAP VTFKGFRE.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Mad2L1Bp Human
  • View Data Sheet

    Description:

    MAD2 Mitotic Arrest Deficient-Like 1 Human Recombinant

    Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2.

    Product # :

    PRO-934

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    • description
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    Description

    MAD2L1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 25.7kDa.MAD2L1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    MAD2L1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      MAD2L1-binding protein (MAD2L1) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is vital for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

    • Synonyms

      Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MALQLSREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG LTLLVTTDLE LIKYLNNVVE QLKDWLYKCS VQKLVVVISN IESGEVLERW QFDIECDKTA KDDSAPREKS QKAIQDEIRS VIRQITATVT FLPLLEVSCS FDLLIYTDKD LVVPEKWEES GPQFITNSEE VRLRSFTTTI HKVNSMVAYK IPVND.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Mad2L1 Human

About MAD2:

MAD2, standing for mitotic arrest deficient 2, is a spindle checkpoint protein that plays an integral role in the human body. The makeup and biological functionality of this protein is integral to the way the body works, and plays a massive role in the way we live our lives,

MAD2 Mechanism
The mechanism that prevents separation and transition into anaphase is known as spindle checkpoint. This exists primarily as a safeguard against chromosome segregation errors, and delays anaphase until all of the sister chromatid pairs have correctly attached.

MAD2 Interactions
There are several interactions that MAD2 shares with others, specifically, binding with Cdc20, in a vital protein-to-protein interaction. This happens until all chromosomes are aligned at the metaphase plate. In addition to this interaction, MAD2 also interacts with Chk1, which is integral across multiple points of the cell cycle process, and this is one of the key interactions that plays a massive role in the process.

MAD2 Function
The primary function of MAD2 is to accumulate at kinetochores, and generate a wait signal that functions to prevent the cell from progressing to the anaphase of the cell cycle until the appropriate moment, and this is integral to the process.
This is integral to the whole process, and there is much to think about in the future by way of things like spindle checkpoint signaling, as well as the contribution of other proteins that play a role in the process.