Karyopherin

Karyopherin

Karyopherin

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  • View Data Sheet

    Description:

    Karyopherin Beta 1 Human Recombinant

    Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97kDa subunit, PTAC97, KPNB1, NTF97.

    Product # :

    PRO-1001

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    Description

    KPNB1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 899 amino acids (1-876 a.a.) and having a molecular mass of 99.6kDa. KPNB1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    KPNB1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 0.1M NaCl.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      KPNB1 is a member of the importin beta family. The KPNB1 protein is engaged in nuclear protein import, either by coupling itself with an adapter protein (e.g., importin-alpha subunit which binds to nuclear localization signals (NLS) in cargo substrates), or by functioning autonomously as a nuclear transport receptor (acts as NLS receptor, docking of the importin/substrate complex to the nuclear pore complex).

    • Synonyms

      Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97kDa subunit, PTAC97, KPNB1, NTF97.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMELITIL EKTVSPDRLE LEAAQKFLER AAVENLPTFL VELSRVLANP GNSQVARVAA GLQIKNSLTS KDPDIKAQYQ QRWLAIDANA RREVKNYVLQ TLGTETYRPS SASQCVAGIA CAEIPVNQWP ELIPQLVANV TNPNSTEHMK ESTLEAIGYI CQDIDPEQLQ DKSNEILTAI IQGMRKEEPS NNVKLAATNA LLNSLEFTKA NFDKESERHF IMQVVCEATQ CPDTRVRVAA LQNLVKIMSL YYQYMETYMG PALFAITIEA MKSDIDEVAL QGIEFWSNVC DEEMDLAIEA SEAAEQGRPP EHTSKFYAKG ALQYLVPILT QTLTKQDEND DDDDWNPCKA AGVCLMLLAT CCEDDIVPHV LPFIKEHIKN PDWRYRDAAV MAFGCILEGP EPSQLKPLVI QAMPTLIELM KDPSVVVRDT AAWTVGRICE LLPEAAINDV YLAPLLQCLI EGLSAEPRVA SNVCWAFSSL AEAAYEAADV ADDQEEPATY CLSSSFELIV QKLLETTDRP DGHQNNLRSS AYESLMEIVK NSAKDCYPAV QKTTLVIMER LQQVLQMESH IQSTSDRIQF NDLQSLLCAT LQNVLRKVQH QDALQISDVV MASLLRMFQS TAGSGGVQED ALMAVSTLVE VLGGEFLKYM EAFKPFLGIG LKNYAEYQVC LAAVGLVGDL CRALQSNIIP FCDEVMQLLL ENLGNENVHR SVKPQILSVF GDIALAIGGE FKKYLEVVLN TLQQASQAQV DKSDYDMVDY LNELRESCLE AYTGIVQGLK GDQENVHPDV MLVQPRVEFI LSFIDHIAGD EDHTDGVVAC AAGLIGDLCT AFGKDVLKLV EARPMIHELL TEGRRSKTNK AKTLATWATK ELRKLKNQA.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Kpnb1 Human
  • View Data Sheet

    Description:

    Karyopherin Alpha 2 Human Recombinant

    Karyopherin subunit alpha 2 (RAG cohort 1 importin alpha 1), RCH1, QIP2, IPOA1, SRP1 alpha, RAG cohort protein 1, importin alpha 1, importin subunit alpha-2, importin-alpha-P1, pendulin.

    Product # :

    PRO-1032

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    • description
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    Description

    KPNA2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 553 amino acids (1-529) and having a molecular mass of 60.5kDa.KPNA2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The KPNA2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      KPNA2 belongs to the karyopherin alpha family. KPNA2 has a vital part in the nuclear import of proteins with a conventional nuclear localization signal (NLS).
      In vitro, KPNA2 bound specifically and directly to substrates having a simple or bipartite NLS motif. KPNA2 stimulated docking of import substrates to the nuclear envelope and together with Ran reconstituted complete nuclear protein import.

    • Synonyms

      Karyopherin subunit alpha 2 (RAG cohort 1 importin alpha 1), RCH1, QIP2, IPOA1, SRP1 alpha, RAG cohort protein 1, importin alpha 1, importin subunit alpha-2, importin-alpha-P1, pendulin.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQ MLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNII RAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWAL GNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTL VRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDE QTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKE AVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEE CGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Kpna2 Human

About KPN / Karyopherin:

Karyopherins are proteins that help eukaryotic cells transport molecules between their cytoplasm and nucleus. The karyoplasm is the fluid that surrounds the nucleus (or nucleoplasm). In most cases, karyopherin-mediated transport occurs through nuclear pores, which serve as a portal into and out of the nucleus. To pass through the nuclear pore, most proteins need karyopherins.

Karyopherins Function
Karyopherins may act as imports (helping proteins enter the nucleus) or exports (helping proteins leave the nucleus) (i.e., helping proteins get out of the nucleus). The transporter classification database belongs to the nuclear pore complex family (TCDB). The Ran gradient provides energy for transportation.

KPN Structure
Several karyopherins are sequestered in stress granules, cytoplasmic aggregates of ribonucleoprotein complexes, as they avoid shuttling between the nucleus and the cytoplasm.

Karyopherins Mechanism
Importin beta is a form of karyopherin that helps cargo proteins get into the nucleus. First, it binds importin alpha, a form of karyopherin that binds the cargo protein in the cytoplasm, before the cargo protein is imported into the nucleus through the nuclear pore driven by energy from the Ran gradient. The cargo separates from the karyopherins once within the nucleus.
Without the aid of the importin alpha adapter protein, Importin beta can transport proteins into the nucleus.
Small RNAs are exported using Ran-GTP for directionality by karyopherin transport receptors, but mRNA is exported using a different mechanism that involves various quality controls. mRNA is exported in the form of very large mRNP complexes, which begin to form during RNA processing when the transcription export (TREX) complex binds to the mRNA. These mRNP complexes dock on the pore's inner surface, where they are subjected to quality control by the exosome and other surveillance activities.