Inhibitor of DNA Binding

Inhibitor of DNA Binding

Inhibitor of DNA Binding

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  • View Data Sheet

    Description:

    Inhibitor of DNA Binding 2 Human Recombinant

    DNA-binding protein inhibitor ID-2, bHLHb26, GIG8, ID2A, ID2H, MGC26389, Class B basic helix-loop-helix protein 26, Inhibitor of DNA binding 2.

    Product # :

    PRO-1383

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    • description
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    Description

    ID2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids (1-134 a.a.) and having a molecular mass of 17kDa. ID2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    ID2 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.

    Purity

    Greater than 85% as determined by SDS-PAGE.

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    • Introduction

      Inhibitor of DNA Binding 2 (ID2) is a part of the inhibitor of DNA binding family, whose members are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative way by suppressing their heterodimerization partners through the HLH domains. ID2 play a role in negatively regulating cell differentiation and may be an inhibitor of tissue-specific gene expression.

    • Synonyms

      DNA-binding protein inhibitor ID-2, bHLHb26, GIG8, ID2A, ID2H, MGC26389, Class B basic helix-loop-helix protein 26, Inhibitor of DNA binding 2.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF PSELMSNDSK ALCG.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Id2 Human
  • View Data Sheet

    Description:

    Inhibitor of DNA Binding 1 Human Recombinant

    bHLHb24, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, ID1.

    Product # :

    PRO-1426

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    Description

    ID1 Human Recombinant produced in E. coli is a single polypeptide chain containing 178 amino acids (1-155) and having a molecular mass of 18.5kDa. ID1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The ID1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      Inhibitor of DNA Binding 1 (ID1) is a helix-loop-helix protein which can form heterodimers with members of the basic HLH family of transcription factors. ID1 is lacking DNA binding activity and thus can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. ID1 participates in cell growth, senescence, and differentiation.

    • Synonyms

      bHLHb24, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, ID1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMKVASGS TATAAAGPSC ALKAGKTASG AGEVVRCLSE QSVAISRCAG GAGARLPALL DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL ELNSESEVGT PGGRGLPVRA PLSTLNGEIS ALTAEAACVP ADDRILCR.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Id1 Human

About ID / Inhibitor of DNA Binding:

Inhibitor of DNA-binding/differentiation proteins, also known as ID proteins, are a family of proteins that heterodimerize with basic helix-loop-helix (bHLH) transcription factors to inhibit DNA binding of bHLH proteins. ID proteins also contain the HLH-dimerization domain. However, they lack the basic DNA-binding domain, thus regulating bHLH transcription factors when they heterodimerize with bHLH proteins.

ID Function
ID proteins regular development where they function to prevent premature differentiation of stem cells. ID proteins regulate the timing of differentiation of stem cells during development by inhibiting E-protein dimer formations that cause differentiation. ID proteins also promote cell cycle progression, delaying senescence, and assist with cell migration.
Inappropriate regulation of ID proteins in differentiated cells can contribute to tumorigenesis, also known as carcinogenesis or the formation of cancer where normal cells are transformed into cancer cells. When ID proteins are overexpressed, it enhances cell proliferation and cells become insensitive to growth factor depletion.

Inhibitor of DNA Binding Subtypes
Humans express four types of Id proteins called ID1, ID2, ID3, and ID4.
Publications have shown that ID1 can be used to mark endothelial progenitor cells which are critical to tumour growth and angiogenesis. Targeting ID1 can result in decreased tumour growth which could potentially be used to design a novel cancer therapy.
Studies have recorded the phenotypic effects of each ID gene when knocked out in mice:
●A knockout of ID1 showed a defect in T-cell migration.
●A knockout of ID2 showed that 25% of mice died perinatally while the born lacked lymph nodes and showed defects in mammary proliferation.
●A knockout of ID3 showed normal development, but they had a defect in B-cell proliferation.
●A knockout of ID4 showed neural defects and premature differentiation.
●A knockout of both ID1 and ID3 resulted in embryonic lethality due to brain haemorrhaging and abnormalities in cardiac development.