I-309 (CCL1)

I-309 (CCL1)

I-309 (CCL1)

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  • View Data Sheet

    Description:

    I-309 (CCL1) Human Recombinant, His Tag

    Small inducible cytokine A1, CCL1, T lymphocyte-secreted protein I-309, chemokine (C-C motif) ligand 1, P500, SISe, TCA3, I-309, SCYA1.

    Product # :

    CHM-254

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    Shipped with Ice Packs

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    • description
    • source
    • formulation
    • purity
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    Description

    I-309 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 94 amino acids (24-96 a.a.) and having a molecular mass of 10.8kDa. The I-309 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The I-309 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 2mM DTT, 10% glycerol and 50mM NaCl.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.

    • Synonyms

      Small inducible cytokine A1, CCL1, T lymphocyte-secreted protein I-309, chemokine (C-C motif) ligand 1, P500, SISe, TCA3, I-309, SCYA1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    I 309 Human His
  • View Data Sheet

    Description:

    I-309 Human Recombinant (CCL1)

    Small inducible cytokine A1, CCL1, T lymphocyte-secreted protein I-309, chemokine (C-C motif) ligand 1, P500, SISe, TCA3, I-309, SCYA1.

    Product # :

    CHM-312

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    Quantity :

    Shipping Method :

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    Shipped at Room temp

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    More Info

    • description
    • source
    • formulation
    • purity
    • biological activity
    • More Info

    Description

    I-309 Human Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8504 Dalton. The I-309 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The CCL1 protein was lyophilized with no additives.

    Purity

    Greater than 99.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    Biological Activity

    The Biological activity is calculated by its ability to chemoattract human T cells at 10-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

    More Info

    • Introduction

      Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. This chemokine resides in a large cluster of CC chemokines on human chromosome 17.

    • Synonyms

      Small inducible cytokine A1, CCL1, T lymphocyte-secreted protein I-309, chemokine (C-C motif) ligand 1, P500, SISe, TCA3, I-309, SCYA1.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized I-309 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized I-309 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    • Amino Acid Sequence

      The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Ser-Met-Gln.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    I 309 Human

About CCL1/ I-309: 

Mechanism
● CCL1 stands for “chemokine (C-C motif) ligand 1”.
● CCL1 is a small glycoprotein.
● It is secreted by activated T-cells.
● CCL1 belongs to the chemokines family of cytokines. Chemokines are known for their role in the inflammatory response and immunoregulatory processes.
● CCL1 is part of a larger cluster of similar chemokines, which are located on human chromosome 17.
● CCL1 is structurally related to the CXC group of proteins.

CCL1 is also known as:
❖ I-309; T lymphocyte-secreted protein
❖ Inflammatory cytokine I-309
❖ Small inducible cytokine A1

Function
● CCL1 is considered to be an antimicrobial gene.
● CCL1 has been found present in a number of organs, with the highest presentation found in the colon.
● Other organs where CCL1 is present at significant levels include the brain, the testes, appendix, and gallbladder.
● CCL1 has an established role in cellular aggregate formation in vitro.
● CCL1 has been found in synovial fluids and macrophages in those suffering from juvenile idiopathic arthritis.

Interactions
● CCL1 is able to attract a number of cells by interacting with CCR8; a cell surface chemokine receptor.
● The cells that CCL1 attracts include: monocytes, immature B cells, dendritic cells, and NK cells.
● It is notable that the list of cells that experience chemotactic activity does not include neutrophils.

Significant notes
● Studies have been conducted regarding elevated levels of CCL1 for people suffering from bullous pemphigoid, an ulcerative disorder that is thought to be autoimmune in nature.
● Elevated levels of CCL1 correlate with higher levels of pruritus in patients with cutaneous T-cell lymphoma.
● The interaction of CCL1 with receptor CCR8 have been found to have an influential role in lymphocytic recruitment in IgG4-SC/AIP. It is believed that this leads to duct-centered inflammation and obliterative phlebitis. This has been noted in patients with type 1 autoimmune pancreatitis.
● Peritoneal adhesions are a significant complication in the aftermath of surgery. Studies have found that Anti-CCL1 mAb or a disruption of the CCR8 receptor has shown decreased peritoneal adhesions.
● CCL1 overexpression has been found to enhance granuloma formation following mycobacterial treatment. It has been speculated this happens due to enhancing endoplasmic reticulum stress.
● It has also been theorized that the activation of CCR8 by its ligand, CCL1, may play an important role in a number of illnesses. Illnesses that may benefit from further investigation into this relationship include asthma, multiple sclerosis, and cancers.