Hairy and Enhancer of Split

Hairy and Enhancer of Split

Hairy and Enhancer of Split

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  • View Data Sheet

    Description:

    Hairy and Enhancer of Split 7 Human Recombinant

    Hairy and enhancer of split 7, bHLHb37, SCDO4, Transcription factor HES-7, HES7, hHes7, Class B basic helix-loop-helix protein 37, bHLH factor Hes7.

    Product # :

    PRO-1306

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    • description
    • source
    • formulation
    • purity
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    Description

    HES7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 253 amino acids (1-230) and having a molecular mass of 27kDa. HES7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The HES7 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      HES7 belongs to the Hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of the HES7 is set by Notch signaling. The protein serves as a transcriptional repressor, and is involved in correct patterning of the axial skeleton. A mutation in HES7 has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been discovered for HES7.

    • Synonyms

      Hairy and enhancer of split 7, bHLHb37, SCDO4, Transcription factor HES-7, HES7, hHes7, Class B basic helix-loop-helix protein 37, bHLH factor Hes7.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMVTRDRA ENRDGPKMLK PLVEKRRRDR INRSLEELRL LLLERTRDQN LRNPKLEKAE ILEFAVGYLR ERSRVEPPAA AAPGVPRSPV QDAEALASCY LSGFRECLLR LAAFAHDASP AARAQLFSAL HGYLRPKPPR PKPVDPRPPA PRPSLDPAAP ALGPALHQRP PVHQGHPSPR CAWSPSLCSP RAGDSGAPAP LTGLLPPPPP PHRQDGAPKA PLPPPPAFWR PWP.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Hes7 Human
  • View Data Sheet

    Description:

    Hairy and Enhancer of Split 2 Human Recombinant

    bHLHb40, hairy and enhancer of split 2, Class B basic helix-loop-helix protein 40, HES2.

    Product # :

    PRO-1649

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    • description
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    Description

    HES2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-173 a.a.) and having a molecular mass of 20.9kDa.HES2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    HES2 protein solution (1mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.4M Urea and 10% glycerol.

    Purity

    Greater than 80.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Hairy and enhancer of split 2 (HES2) belongs to the HES family and comprised of one basic helix-loop-helix (bHLH) domain and one orange domain. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. Activation of Notch signaling pathway initiates activation of HES family genes throughout the interaction between Notch intracellular domain and RBPSUH (CSL). HES2 is expressed in placenta, pancreatic cancer, colon cancer with RER, cervical cancer, and in head and neck tumors.

    • Synonyms

      bHLHb40, hairy and enhancer of split 2, Class B basic helix-loop-helix protein 40, HES2.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMGLPRRA GDAAELRKSL KPLLEKRRRA RINQSLSQLK GLILPLLGRE NSNCSKLEKA DVLEMTVRFL QELPASSWPT AAPLPCDSYR EGYSACVARL ARVLPACRVL EPAVSARLLE HLWRRAASAT LDGGRAGDSS GPSAPAPAPA SAPEPASAPV PSPPSPPCGP GLWRPW.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Hes2 Human

About HES / Hairy and Enhancer of Split:

Hairy and Enhancer of Split, also known as the HES2 or HES7 gene, is a member of the Basic helix-loop-helix proteins group that is found at chromosome 17 of the human body at position 13.1, with a start code of 8,120,592 bp and end code of 8,124,106 bp.
The Hairy and Enhance of Split gene is also known as SCDO4, bHLHb37, hes family bHLH transcription factor 7, HES7 gene. Its Entrez ID is 84667 and its Ensembl genome database project coding is ENSG00000179111. The molecular weight is 24898.78.

HES Function
Expressed cyclically in the presomitic mesoderm, it is a part of the Notch signalling pathway and the self-regulated gene, which can bind to its own promote, is involved in the segmentation of somites. When the HES7 gene is mutated, it can lead to Spondylocostal dysostosis, as well as problems with the heart, ribs, and spine.

Hairy and Enhancer of Split Mechanism
HES7 has 62 known orthologues and expression rates can fluctuate significantly over oscillating two-hour cycles, which are regulated by a negative feedback loop. It, subsequently, represses the expression of N-box or E-box containing genes, as well as genes that have an E47 transcription factor. The function of the HES7 gene is determined primarily by the protein’s half-life. Inaccuracy of expression over the two-hour cycles is the activator for mutations.
In addition to the Notch signalling pathway, the bHLH-type repressor gene additionally shows a PI3K-Akt signaling pathway while it is capable of protein dimerization activity, as well as the factor binding properties.