Gastrokine

Gastrokine

Gastrokine

Name

Description

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  • View Data Sheet

    Description:

    Gastrokine 3 Human Recombinant

    Gastrokine 3 Pseudogene, Gastrokine-3, GKN3P.

    Product # :

    PRO-2033

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    Description

    GKN3P Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Met21-Leu181) containing 171 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 19.4kDa.

    Source

    Escherichia Coli.

    Formulation

    GKN3P filtered (0.4µm) solution at a concentration of 0.3mg/ml in 0.03M acetate buffer, pH 4.0 and 20% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Gastrokine 3 (GKN3P) is a Pseudogene, which May inhibit gastric epithelial cell proliferation.

    • Synonyms

      Gastrokine 3 Pseudogene, Gastrokine-3, GKN3P.

    • Physical Appearance

      Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MKHHHHHHASMMNIRFNHPL YGSFGTQIIH IGAFQGMVSI RDNNIFSEWD GILDYKNALL VAKVFNKMAC VLARMDKAVF PSLDDISKAL DKQAFKYYPS TRGLTYTVLP SWVKNLAQYG KPIKNMCRDD PTYFAQQQKE GTALAIDSNS CFEIQLLSFM GLFICGETPG L.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Gkn3P Human
  • View Data Sheet

    Description:

    Gastrokine 2 Human Recombinant

    Gastrokine-2, Blottin, Down-regulated in gastric cancer, Trefoil factor interactions(z) 1, GKN2, BLOT, GDDR, TFIZ1, PRO813, BRICD1B, VLTI465.

    Product # :

    PRO-1588

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    Description

    Gastrokine 2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 21-184) containing 174 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 19.6kDa (calculated).

    Source

    Escherichia Coli.

    Formulation

    GKN2 filtered (0.4 µm) and lyophilized from 0.5mg/ml in 0.05M Acetate buffer pH-4.0.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Gastrokine-2 (GKN2) is expressed in the gastric mucosa. GKN2 forms a heterodimer with TFF1- disulfide linked. GKN2 interacts with TFF2.

    • Synonyms

      Gastrokine-2, Blottin, Down-regulated in gastric cancer, Trefoil factor interactions(z) 1, GKN2, BLOT, GDDR, TFIZ1, PRO813, BRICD1B, VLTI465.

    • Physical Appearance

      Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

    • Solubility

      It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. GKN2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

    • Amino Acid Sequence

      MKHHHHHHASYEVFNIISPS NNGGNVQETV TIDNEKNTAI INIHAGSCSS TTIFDYKHGY IASRVLSRRA CFILKMDHQN IPPLNNLQWY IYEKQALDNM FSSKYTWVKY NPLESLIKDV DWFLLGSPIE KLCKHIPLYK GEVVENTHNV GAGGCAKAGL LGILGISICA DIHV.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Gkn2 Human
  • View Data Sheet

    Description:

    Gastrokine 1 Human Recombinant

    BRICHOS domain containing 1, 18 kDa antrum mucosa protein, gastrokine 1, Protein CA11, AMP-18, BRICD1, foveolin, FOV.

    Product # :

    PRO-1206

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    Description

    GKN1 Human Recombinant produced in E. coli is a single polypeptide chain containing 223 amino acids (1-199) and having a molecular mass of 24.5 kDa.GKN1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The GKN1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      Gastrokine-1 (GKN1) is a member of the gastrokine family. GKN1 has mitogenic activity and is involved in preserving the integrity of the gastric mucosal epithelium. GKN1 is down-regulated in human gastric cancer tissue in comparison to normal gastric mucosa. GKN1 is expressed in the stomach; however no expression is detected in cancer tissue or gastric cancer cell lines.

    • Synonyms

      BRICHOS domain containing 1, 18 kDa antrum mucosa protein, gastrokine 1, Protein CA11, AMP-18, BRICD1, foveolin, FOV.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSH MLAYSS VHCFREDKMK FTIVFAGLLG VFLAPALANY NINVNDDNNN AGSGQQSVSV NNEHNVANVD NNNGWDSWNS IWDYGNGFAA TRLFQKKTCI VHKMNKEVMP SIQSLDALVK EKKLQGKGPG GPPPKGLMYS VNPNKVDDLS KFGKNIANMC RGIPTYMAEE MQEASLFFYS GTCYTTSVLW IVDISFCGDT VEN.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Gkn1 Human

About GKN / Gastrokine:

Gastrokine, also known as Gastrokine-1 or GKN1, is a naturally-occurring protein within the human body and is a member of the CCDS set and found in the stomach. The protein of 181-184 amino acids is composed of a hydrophobic signal peptide, a BRICHOS domain, and a COOH-terminal domain.

GKN Function
GKN1 is a protein that helps maintain and regulate gastric homeostasis by suppressing tumors and inhibiting inflammation. It is expressed on the surface mucosa cells located in the gastric antrum and fundus, and is decreased when infected by Helicobacter pylori or suffering from inflammation. As a member of the BRICHOS superfamily of proteins, which includes proteins linked to cancer development, it is additionally found to be downregulated in gastric cancer. The protein has subsequently become a potential theragnostic target for gastric cancer.

Gastrokine Mechanism
In healthy patients, the Gastrokine protein is expressed on the surface of the cells but not at depth. It inhibits the gastrin/cholecystokinin type B receptor while activating both the p16/Rb and p21 signaling pathways leading the cells to senescence. Gastrokine-1 also prevents epithelial gastric cells from developing into cancerous cells by regulating cytokines attributed to the gastric carcinogenesis. It brings an increase to interleukin (IL)-8 and IL-17 and reduction to (NF)-κB, IL-6 and IL-10.
GKN1’s other aliases include AMP18, BRICD1, CA11, FOV, and foveolin. Its Entrez coding is 56287 while its Ensembl genome database project code is ENSG00000169605. It is joined in the gastrokine family by GKN2 and GKN3, with the latter only being found in the previous decade.