Fragile Histidine Triad

Fragile Histidine Triad

Fragile Histidine Triad

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    Description:

    Fragile Histidine Triad Human Recombinant, GST Tag

    Bis(5'-adenosyl)-triphosphatase, EC 3.6.1.29, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, AP3A hydrolase, AP3Aase, Fragile histidine triad protein, FHIT, FRA3B.

    Product # :

    PRO-297

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    Description

    FHIT Human Recombinant full length protein expressed in E.coli, shows a 43 kDa band on SDS-PAGE. The FHIT is fused to GST-Tag and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    FHIT in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.

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    • Introduction

      FHIT is a member of the histidine triad gene family. FHIT gene encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. FHIT gene includes the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and abnormal transcripts of this gene. The FHIT protein is a tumor suppressor with reduced or no expression in numerous types of cancer. FHIT may also act as a tumor suppressor in normal cells. Alterations and deletions of the FHIT (Fragile Histidine Triad) gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach, and pancreas. The expression of FHIT is more often lost in cancers of individuals with familial mutations causing deficiency in DNA repair genes such as BRCA1, BRCA2 and MSH2. In vitro FHIT functions as a hydrolase that cleaves diadenosine triphosphate (Ap3A) to ADP and AMP. The FHIT -Ap3A enzyme-substrate complex seems to be the tumor suppressor signal. The restoration of FHIT expression in FHIT -deficient cancer cells leads to apoptosis, involving the intrinsic caspase pathway, in cancer-derived cells and in tumor xenografts.

    • Synonyms

      Bis(5'-adenosyl)-triphosphatase, EC 3.6.1.29, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, AP3A hydrolase, AP3Aase, Fragile histidine triad protein, FHIT, FRA3B.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles.

    • Applications

      • ELISA
      • Inhibition Assays
      • Western Blotting.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Fhit Human Gst
  • View Data Sheet

    Description:

    Fragile Histidine Triad Human Recombinant

    EC 3.6.1.29, Dinucleosidetriphosphatase, Bis (5''-adenosyl)-triphosphatase , AP3Aase, AP3A hydrolase, Diadenosine 5'',5''''''-P1,P3-triphosphate hydrolase, FRA3B.

    Product # :

    PRO-828

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    • description
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    Description

    FHIT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids (1-147 a.a.) and having a molecular mass of 17.9 kDa. FHIT protein is fused to an 8 amino acid His tag at C-terminus and is purified by standard chromatography.

    Source

    Escherichia Coli.

    Formulation

    FHIT Human solution containing 20mM Tris-HCl pH-8 & 10% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      FHIT enzyme cleaves adenosine 5'' PPP 5'' A to yield AMP and ADP. FHIT gene includees the regular fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are highly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT functions as a tumor suppressor and physically relates with ubiquitin conjugating enzyme 9.

    • Synonyms

      EC 3.6.1.29, Dinucleosidetriphosphatase, Bis (5''-adenosyl)-triphosphatase , AP3Aase, AP3A hydrolase, Diadenosine 5'',5''''''-P1,P3-triphosphate hydrolase, FRA3B.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Fhit Human

About FHIT / Fragile Histidine Triad:

The fragile histidine triad protein (FHIT) enzyme is encoded by the FHIT gene in humans. It is also known as the human accelerated region and may have played an important role in distinguishing humans from apes.

FHIT Expression
The FHIT protein is a tumor suppressor that is expressed infrequently or not at all in many forms of cancer. FHIT expression is more commonly lost in cancers of people with familial mutations that cause deficiencies in DNA repair genes including BRCA1 and BRCA2 as well as MSH2.

Fragile Histidine Triad Function
In vitro, FHIT acts as a hydrolase, converting adenosine triphosphate (Ap3A) to ADP and AMP. The tumor suppressor signal appears to be the FHIT-Ap3A enzyme-substrate complex. In cancer-derived cells and tumor xenografts, restoring FHIT expression in FHIT-deficient cancer cells triggers apoptosis via the intrinsic caspase pathway.

FHIT Family
FHIT is a member of the broad HIT family of proteins characterized by the histidine triad motif, HxHxHxx, and is comparable to a yeast enzyme, diadenosine tetraphosphate (Ap4A) hydrolase (where x is a hydrophobic residue).

Fragile Histidine Triad Structure
The FHIT gene stretches over 1.6 Mb of genomic DNA and is made up of ten exons.
The FHIT gene encodes a 1.1 kb mRNA that is generated at low levels in the majority of tissues. FHIT includes the common fragile site FRA3B, where carcinogen-induced damage may result in deletions, translocations, and erroneous transcripts. This gene's erroneous transcripts have been detected in about half of all esophageal carcinomas, stomach carcinomas, and other carcinomas. On chromosome 1, there is a pseudogene with sequences that are almost similar to the 5'UTR of FHIT.