Cysteine-Rich Secretory Protein

Cysteine-Rich Secretory Protein

Cysteine-Rich Secretory Protein

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    Description:

    Cysteine-Rich Secretory Protein 2 Human Recombinant

    Cysteine-Rich Secretory Protein 2, Cancer/Testis Antigen 36, TPX1, Testis Specific Protein 1 (Probe H4-1 P3-1), Testis-Specific Protein TPX-1, CRISP-2, GAPDL5, TSP1, CT36, Glyceraldehyde-3-Phosphate Dehydrogenase-Like 5, CRISP2.

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    PRO-2030

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    Description

    CRISP2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (22-243) containing 232 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 26.32kDa.

    Source

    Escherichia Coli.

    Formulation

    CRISP2 filtered (0.4µm) solution at a concentration of 0.3mg/ml in 50mM acetate buffer, pH 4, 5mM DTT and 20%glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

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    • Introduction

      Cysteine-Rich Secretory Protein 2 (CRISP2) regulates some ion channels activity and thereby regulates calcium fluxes during sperm capacitation. Amongst its related pathways are p53 signaling. Diseases related with CRISP2 include epididymitis and orchitis.

    • Synonyms

      Cysteine-Rich Secretory Protein 2, Cancer/Testis Antigen 36, TPX1, Testis Specific Protein 1 (Probe H4-1 P3-1), Testis-Specific Protein TPX-1, CRISP-2, GAPDL5, TSP1, CT36, Glyceraldehyde-3-Phosphate Dehydrogenase-Like 5, CRISP2.

    • Physical Appearance

      Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MKHHHHHHASKDPAFTALLT TQLQVQREIV NKHNELRKAV SPPASNMLKM EWSREVTTNA QRWANKCTLQ HSDPEDRKTS TRCGENLYMS SDPTSWSSAI QSWYDEILDF VYGVGPKSPN AVVGHYTQLV WYSTYQVGCG IAYCPNQDSL KYYYVCQYCP AGNNMNRKNT PYQQGTPCAG CPDDCDKGLC TNSCQYQDLL SNCDSLKNTA GCEHELLKEK CKATCLCENK IY.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Crisp2 Human
  • View Data Sheet

    Description:

    Cysteine-Rich Secretory Protein 1 Human Recombinant

    Cysteine-rich secretory protein 1, ARP, CRISP-1, AEGL1, Acidic epididymal glycoprotein-like 1, HSCRISP1D, HSCRISP1G, HUMARP, AEG-like protein, cysteine-rich secretory protein-1 delta.

    Product # :

    PRO-473

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    • description
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    Description

    The CRISP1 Protein Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (Lys 22 - Lys 249) having a molecular mass of 27kDa.CRISP1 is fused to 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The protein was lyophilized from a 0.5mg/ml solution containing 20mM TRIS, 50mM NaCl, pH 7.5.

    Purity

    Greater than 85% as determined by SDS-PAGE.

    More Info

    • Introduction

      Insemination involves of a series of specific cell-cell interactions concluding in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion take place through the interface of complementary molecules which are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene belongs to the cysteine-rich secretory protein (CRISP) family. CRISP1 is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head, where it takes part in sperm-egg fusion.

    • Synonyms

      Cysteine-rich secretory protein 1, ARP, CRISP-1, AEGL1, Acidic epididymal glycoprotein-like 1, HSCRISP1D, HSCRISP1G, HUMARP, AEG-like protein, cysteine-rich secretory protein-1 delta.

    • Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Lyophilized CRISP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CRISP1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized CRISP1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    • Amino Acid Sequence

      MKHHHHHHAS KKKSARDQFN KLVTDLPNVQ EEIVNIHNAL RRRVVPPASN MLKMSWSEEA AQNARIFSKY CDMTESNPLE RRLPNTFCGE NMHMTSYPVS WSSVIGVWYS ESTSFKHGEW TTTDDDITTD HYTQIVWATS YLIGCAIASC RQQGSPRYLY VCHYCHEGND PETKNEPYKT GVPCEACPSN CEDKLCTNPC IYYDEYFDCD IQVHYLGCNH STTILFCKAT CLCDTEIK

    • Applications

      Western blotting, ELISA.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Crisp1 Human

About CRISP / Cysteine-Rich Secretory Protein:

Cysteine-rich secretory proteins are also often referred to as “CRISPs”. They are a group of glycoproteins that are a subgroup of the CRISP, antigen 5 and Pr-1 (CAP) protein superfamily. All cysteine-rich secretory proteins contain a domain related to the ShK toxins.

CRISP Structure
These proteins are glycoproteins. They have a number of carbohydrates glycans covalently attached to amino acid side-chains on their surface via glycosylation. In regards to structure, cysteine-rich secretory proteins contain two domains which are joined by a hinge region. The larger of these two domains is a CAP-like 'Pathogenesis-related 1' domain. This is commonly referred to as “PR-1”. The smaller region is an ShK-like 'Cysteine-Rich Domain'. This is also referred to as a “CRD”. The primary structure is also rich in cysteine that form disulfide bonds, particularly in the hinge region and CRD.

Cysteine-Rich Secretory Protein Function
Cysteine-rich secretory proteins play a major role in the functioning of the mammalian reproductive system. They are also found in a number of different snake venoms. Within these venoms, they can be found to inhibit both smooth muscle contraction and cyclic nucleotide-gated ion channels.
When it comes to mammalian reproduction, cysteine-rich secretory proteins can be found in the testes and epididymis of mammals. They are also involved in the process of fertilisation and the spermatogenesis process - this is the development of the spermatozoa in the testis. Here, the CRISP2 protein is incorporated into the acrosome where it is believed to be involved in the adhesion of germ cells with Sertoli cells. CRISP2 can also form part of the sperm tail. In the sperm tail, CRISP2 is believed to help regulate flagellar beating. Proteins CRISP1 and CRISP4 can also be found in the epididymis, where they are also incorporated within the spermatozoa as it matures. Then, protein CRISP3 is found in seminal fluid. It is excreted from the prostate. CRISP3’s function is unknown.