Cofilin

Cofilin

Cofilin

Name

Description

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  • View Data Sheet

    Description:

    Cofilin-2 Human Recombinant

    Cofilin-2, Cofilin- muscle isoform, CFL2, NEM7.

    Product # :

    PRO-912

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    Quantity :

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    • description
    • source
    • formulation
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    Description

    CFL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 186 amino acids (1-166 a.a.) and having a molecular mass of 20.9kDa.CFL2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CFL2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 50mM NaCl.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      CFL2 protein is a member of the actin-binding proteins ADF family which contains 1 ADF-H domain. Cofilin is a broadly distributed intracellular actin-modulating protein which binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in the CFL2 gene are the cause of nemaline myopathy type 7 (NEM7).

    • Synonyms

      Cofilin-2, Cofilin- muscle isoform, CFL2, NEM7.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cfl2 Human
  • View Data Sheet

    Description:

    Cofilin-1 Human Recombinant

    CFL-1, CFL1, Cofilin1, Cofilin-1, Cofilin, non-muscle isoform, 18 kDa phosphoprotein, p18.

    Product # :

    PRO-591

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    Cofilin1 Human Recombinant (aa 1-166) fused to a 20 aa N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing a total of 186 amino acids and having a molecular mass of 20kDa.

    Source

    Escherichia Coli.

    Formulation

    The protein solution contains 20mM Tris pH-8 and 10% glycerol.

    Purity

    Greater than 95.0% as determined by Analysis by SDS-PAGE.

    More Info

    • Introduction

      Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

    • Synonyms

      CFL-1, CFL1, Cofilin1, Cofilin-1, Cofilin, non-muscle isoform, 18 kDa phosphoprotein, p18.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCLSEDKKNIILE EGKEILVGDV GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLVFIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cfl1 Human

About CFL / Cofilin:

cofilin plays a role in the depolymerization of actin microfilaments.

CFL Structure
Cofilin or CFL as it is also known is a protein similar to destrin that is part of the ADF/cofilin family of small ADP-binding proteins. There are two types of cofilin CFL1, coding for cofilin 1 (non-muscle, or n-cofilin) and CFL2, coding for cofilin 2 (found in muscle: m-cofilin).

Cofilin Function
Cofilin is associated with several functions including cell transcription regulation, cell motility, and muscle contraction. Cofilin is important to these processes because it prompts dynamic instability of the actin microfilaments. This is because cofilin is an actin-binding protein associated with rapid depolymerization of actin microfilaments.
Indeed, cofilin is responsible for the depolymerization at the minus end of filaments, thus preventing their reassembly.

CFL Mechanism
Cofilin prevents filament reassembly in two ways. The first is by depolymerizing old actin filaments. While the second is by creating new ends for polymerization. In essence, what this process does is cause the molecule to twist. This, then adds strain to the molecule and therefore destabilizes it.
This twisting process is then enhanced as the actin-depolymerizing factor then binds to the destabilized F-actin. This is a process that is made possible because the actin-depolymerizing factor can insert the central helix into the cleft between the first and third actin subunits.
This change in the F-actin causes the molecule to twist further and become even more destabilized. Therefore, effectively severing the bond between the actins and effectively depolymerizing the filament.