CEBP

CEBP

CEBP

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  • View Data Sheet

    Description:

    CCAAT/enhancer binding protein C/EBP Gamma Recombinant Human

    CCAAT/enhancer-binding protein gamma, C/EBP gamma, CEBPG, GPE1BP, IG/EBP-1.

    Product # :

    PRO-434

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    CEBP-g Recombinant Human His-Tag fusion protein produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 146 (aa 39-147) and having a molecular mass of 16.5 kDa. The DNA binding domain of CEBP-g was purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The protein contains 20mM Tris-HCl pH7.5, 0.1M NaCl and 5mM b-Mercaptoethanol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      CCAAT/enhancer binding protein(C/EBP) g is a family of transcription factors all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP a,b,g,d, that form homodimers and/or form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBP g may cooperate with Fos to bind PRE- enhancer elements.

    • Synonyms

      CCAAT/enhancer-binding protein gamma, C/EBP gamma, CEBPG, GPE1BP, IG/EBP-1.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMPGG GGKAVAPSKQ SKKSSPMDRN SDEYRQRRER NNMAVKKSRL KSKQKAQDTL QRVNQLKEEN ERLEAKIKLL TKELSVLKDL FLEHAHNLAD NVQSISTENT TADGDN.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cebpg Human
  • View Data Sheet

    Description:

    CCAAT/enhancer binding protein CEBP Alpha Human Recombinant

    CCAAT/enhancer-binding protein alpha, C/EBP alpha, CEBPA, CEBP.

    Product # :

    PRO-433

    Price :

    Quantity :

    Shipping Method :

    Ice Icon

    Shipped with Ice Packs

    Add To Cart

    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    CEBP-a Human Recombinant His-Tag fusion protein produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 126 (aa 270-358) and having a molecular mass of 14.5 kDa. The Human CEBP-a Human is fused to a 37 amino acids His-Tag at N-terminus and is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The protein contains 20mM Tris-HCl pH7.5, 0.1M NaCl and 5mM b-Mercaptoethanol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      CCAAT/enhancer binding protein(C/EBP) a is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP a,b,g,d, that form homodimers and that form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBPs differ significantly in their physiological functions and in their downstream target genes. For example, mice lacking C/EBPa die shortly after birth due to severe hypoglycemia and the absence of glycogen storage in liver, whereas knockout of C/EBPb causes defects in female reproduction.

    • Synonyms

      CCAAT/enhancer-binding protein alpha, C/EBP alpha, CEBPA, CEBP.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGAG KAKKSVDKNS NEYRVRRERN NIAVRKSRDK AKQRNVETQQ KVLELTSDND RLRKRVEQLS RELDTLRGIF RQLPESSLVKAMGNCA.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cebpa Human

About CEBP / CCAAT-Enhancer-Binding Proteins:

These proteins are a six-member transcription factor-family that comprises CEBPs from CEBPα (CEBP alpha) to CEBPζ (CEBPS zeta).
These proteins are heavily involved in gene binding and dimerization for the different regulatory body system functions.

CEBP Mechanism
CEBP uses various mechanisms to regulate its interaction with different factors and proteins: activation, acetylation, phosphorylation, and auto regulation. It uses these complex mechanisms to regulate its expression in different physiological levels regulated by hormones, cytokines, nutrients, and others.

CCAAT-Enhancer-Binding Proteins Structure
All CEBP members bar the CEBPϒ have a functional activation domain that initiates transcription for essential interaction. Four of the family's six elements do not have introns, while the CEBP gamma contains four exons.
These diverse gen combinations within the family have different DNA and RNA binding interactions.
Different types of CEBPs produce different types of polypeptides. It is vital to note the types that make non-functional heterodimers like the LIP from the beta variation.

CEBP Interactions
The presence of CCAAT binds with the CEBP and is present in some gen promoters. The bZip domain, which is highly conserved at the C terminus of CEBP and plays a primary role in DNA binding and dimerization, characterizes the interaction of the CBEP.

CCAAT-Enhancer-Binding Proteins Function
CEBP is an enhanced binding protein that has various uses in the medical field. The primary function of CEBP is to regulate the body's metabolism and inflammation and other types of responses, and build immunity in the body system.
Every kind of CEBPs performs a particular role in proteolysis during the interaction and activation.
CEBP's importance to the nervous system is unmatched as it helps develop the nerve cells and ensure proper functionality. It takes adequate interaction through the different mechanisms to achieve this functionality.