Calponin

Calponin

Calponin

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  • View Data Sheet

    Description:

    Calponin 2 Human Recombinant

    Calponin 2, Calponin H2, Smooth Muscle, Neutral Calponin, calponin-2.

    Product # :

    PRO-1882

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    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    CNN2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids (1-131 a.a) and having a molecular mass of 16.9kDa.CNN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CNN2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, and 10% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      Calponin 2 also known as CNN2 protein is capable of binding actin, calmodulin, troponin C, and tropomyosin, and also function in the structural organization of actin filaments. Furthermore, the interaction of CNN2 with actin inhibits the actomyosin Mg-ATPase activity. CNN2 takes part in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene.

    • Synonyms

      Calponin 2, Calponin H2, Smooth Muscle, Neutral Calponin, calponin-2.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMSSTQFN KGPSYGLSAE VKNRLLSKYD PQKEAELRTW IEGLTGLSIG PDFQKGLKDG TILCTLMNKL QPGSVPKINR SMQNWHQLEN LSNFIKAMVS YGMNPVDLFE ANDLFESGNM TQVQVSLLAL AGKA

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cnn2 Human
  • View Data Sheet

    Description:

    Calponin 1, Basic, Smooth Muscle Human Recombinant

    Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, SMCC, Basic calponin, Sm-Calp, calponin-1.

    Product # :

    PRO-1129

    Price :

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    • description
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    • More Info

    Description

    CNN1 Human Recombinant produced in E. coli is a single polypeptide chain containing 305 amino acids (1-297) and having a molecular mass of 34.2 kDa.CNN1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    The CNN1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 30% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      CNN1 is a member of the calponin family. CNN1 is a thin filament-associated protein which is involved in the regulation and modulation of smooth muscle contraction. CNN1 is able to bind to actin, calmodulin, troponin C and tropomyosin. Prevention of actomyosin Mg-ATPase activity is a result of interaction between calponin and actin.

    • Synonyms

      Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, SMCC, Basic calponin, Sm-Calp, calponin-1.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF MDGLKDGIIL CEFINKLQPG SVKKINESTQ NWHQLENIGN FIKAITKYGV KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER KFEPGKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LGEPAHNHHA HNYYNSALEH HHHHH

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cnn1 Human

About Calponin:

Calponin is a calcium binding protein that is known for binding to a number of things, including actin, myosin, and tropomyosin. In smooth muscle, it also inhibits the ATPase activity of myosin.

Calponin Structure
Calponin typically consists of α-helices and hydrogen bond turns. A binding protein composed of three domains, these domains are Click-23, regulatory domain (referred to as RD), and Calponin Homology (referred to as CH). The CH domain calponin will bind to α-actin and filamin, and when it’s in conjunction with the RD domain, will bind to actin. Calmodulin can sometimes bind weakly to the CH domain if it is activated by calcium, and may also inhibit calponin binding with α-actin. Within the body, it is responsible for a number of things, including binding actin proteins, as well as phospholipids. It’s also in charge of regulating the actin/myosin interaction.

Calponin Function
If the Calponin is phosphorylated by a protein kinase, then the Calponin’s inhibition of the smooth muscle ATPase will be released. It was first discovered in 1986, when Calponin was isolated from both chicken gizzard and bovine aorta and was found to be stable under heat. It is found in humans and other mammals. It is represented in both smooth muscle and non-muscle cell, as well as three different forms that have been identified in the vertebrae.