Calcyphosine

Calcyphosine

Calcyphosine

Name

Description

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  • View Data Sheet

    Description:

    Calcyphosine-Like Human Recombinant

    Calcyphosine-like protein, CAPSL, MGC26610.

    Product # :

    PRO-185

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    • description
    • source
    • formulation
    • purity
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    Description

    CAPSL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (1-208) and having a molecular mass of 26.3 kDa.The CAPSL is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    CAPSL protein at 1mg/ml in 20mM Tris-HCL, pH-8, 0.2M NaCl, 5mM DTT and 20% Glycerol.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      CAPSL is a calcium-binding protein holding two conserved calcium-binding motifs (EF-hands) which are found in a superfamily of calcium sensors and calcium signal modulators. In addition, the CAPSL gene is in the same linkage disequilibrium (LD) block as the IL7R gene and there is well known association between the CAPSL-IL7R locus and type 1 diabetes.

    • Synonyms

      Calcyphosine-like protein, CAPSL, MGC26610.

    • Physical Appearance

      CAPSL is supplied as a sterile filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MAGTARHDRE MAIQAKKKLT TATDPIERLR LQCLARGSAG IKGLGRVFRI MDDDNNRTLD FKEFMKGLND YAVVMEKEEV EELFQRFDKD GNGTIDFNEF LLTLRPPMSR ARKEVIMQAF RKLDKTGDGV ITIEDLREVY NAKHHPKYQN GEWSEEQVFR KFLDNFDSPY DKDGLVTPEE FMNYYAGVSA SIDTDVYFII MMRTAWKL

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Capsl Human
  • View Data Sheet

    Description:

    Calcyphosine Human Recombinant

    Calcyphosin, Calcyphosine, CAPS, CAPS1, MGC126562.

    Product # :

    PRO-117

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    Quantity :

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    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    CAPS Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 209 amino acids (1-189 a.a.) and having a molecular mass of 23.1kDa. The CAPS is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The CAPS solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Calcyphosine (CAPS) is a calcium-binding protein containing four EF-hand domains. CAPS protein was originally identified as thyroid protein p24 which is found in a number of epithelium and in some cells of the central nervous system. CAPS may have a role in the regulation of ion transport. In the thyroid follicular cells, CAPS is synthesized and phosphorylated in response to stimulation by thyrotropin and cAMP agonists.

    • Synonyms

      Calcyphosin, Calcyphosine, CAPS, CAPS1, MGC126562.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MDAVDATMEK LRAQCLSRGA SGIQGLARFF RQLDRDGSRS LDADEFRQGL AKLGLVLDQA EAEGVCRKWD RNGSGTLDLE EFLRALRPPM SQAREAVIAA AFAKLDRSGD GVVTVDDLRG VYSGRAHPKV RSGEWTEDEV LRRFLDNFDS SEKDGQVTLA EFQDYYSGVS ASMNTDEEFV AMMTSAWQL.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Caps Human

About CAPS / Calcyphosine:

Calcyphosine (CAPS) is a gene that encodes proteins in humans. It can also contribute to the regulation of ion transport. Examples of diseases that are linked with CAPS are Malignant Ependymoma and Pediatric Ependymoma. One protein that was described as an essential protein in the dog thyroid, was previously referred to as antigen R2D5 in rabbits. Alternative splicing of CAPS genes generates transcript variants.
Calcyphosine for calcium binding is a target for cyclic AMP and Ca2+ phosphatidylinositol cascades that were first isolated from a dog's thyroid, rabbit and the human brain.

CAPS Function
As a calcium-binding protein with four EF-hand domains, calcyphosine was initially identified as a thyroid protein p24, usually present in epithelium cells and the central nervous system. In the follicular cells of the thyroid, synthesis and phosphorylation of calcyphosine occur as a result of stimulation by thyrotropin and cAMP agonists.

Calcyphosine Mechanism
To confirm whether human recombinant calcyphosine undergoes a calcium-2 (Ca2+) dependent conformational change, researchers loaded purified CAPS onto a column of Phenyl Sepharose in the presence of Ca2+ ions. Elution results showed that CAPS bound to the matrix could be eluted with EGTA. Spectroscopic studies of the calcyphosine monomer’s secondary structure showed that CAPS monomers change their conformation in a calcium-dependent manner.

CAPS Analytical and therapeutic concept
Northern blot analysis has shown that calcyphosine messenger RNA is relatively less abundant in humans than canine thyrocytes. While the exact function of calcyphosine is not fully understood, CAPS can be implicated in cross-signaling between cascades to contact cellular proliferation and differentiation.