Alkaline Phosphatase

Alkaline Phosphatase

Alkaline Phosphatase

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  • View Data Sheet

    Description:

    Alkaline Phosphatase Placental Human Recombinant, BioActive

    3 ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALP, PLAP, Alkaline phosphatase placental type, EC 3.1.3.1, PLAP-1, Alkaline phosphatase Regan isozyme.

    Product # :

    ENZ-1133

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    Description

    ALPP Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 494 amino acids (23-506 a.a.) and having a molecular mass of 53.9kDa. ALPP is expressed with a 10 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    ALPP protein solution (0.5mg/ml) containing Phosphate-Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 2,500unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of pnitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

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    • Introduction

      Placental alkaline phosphatase also known as PLAP is a membranal siaglycoprotein enzyme typicallyfoundin high concentration in syncytiotrophoblasts in the placenta amid the 3th trimester of gestation. The expression of PLAP was at firstconsidered to be onlyin the term placenta, though, a human PLAP-like variant has been found,thathas more than 85% homology with PLAP itself. PLAP is expressed strictly in normal term placenta, endocervix & fallopian tube and in ovarian and proximal gastrointestinal tumors. It is also widely expressed in germ cell tumors and more recently found in seminomas.

    • Synonyms

      3 ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALP, PLAP, Alkaline phosphatase placental type, EC 3.1.3.1, PLAP-1, Alkaline phosphatase Regan isozyme.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADLMIIPVEE ENPDFWNREA AEALGAAKKL QPAQTAAKNL IIFLGDGMGV STVTAARILK GQKKDKLGPE LPLAMDRFPY VALSKTYNVD KHVPDSGATA TAYLCGVKGN FQTIGLSAAA RFNQCNTTRG NEVISVMNRA KKAGKSVGVV TTTRVQHASP AGTYAHTVNR NWYSDADVPA SARQEGCQDI ATQLISNMDI DVILGGGRKY MFRMGTPDPE YPDDYSQGGT RLDGKNLVQE WLAKRQGARY VWNRTELMQA SLDPSVTHLM GLFEPGDMKY EIHRDSTLDP SLMEMTEAAL RLLSRNPRGF FLFVEGGRID HGHHESRAYR ALTETIMFDD AIERAGQLTS EEDTLSLVTA DHSHVFSFGG YPLRGSSIFG LAPGKARDRK AYTVLLYGNG PGYVLKDGAR PDVTESESGS PEYRQQSAVP LDEETHAGED VAVFARGPQA HLVHGVQEQT FIAHVMAFAA CLEPYTACDL APPAGTTDHH HHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Alpp Human
  • View Data Sheet

    Description:

    Alkaline Phosphatase Placental Human Recombinant

    ALP, PLAP, Alkaline phosphatase placental type, EC 3.1.3.1, PLAP-1, Alkaline phosphatase Regan isozyme.

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    ENZ-333

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    Description

    Placental Alkaline Phosphatase Human Recombinant encoding 154-287 amino acids expressed in E.coli, shows a 41kDa band on SDS-PAGE (including GST tag).PLAP Human Recombinant is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    PLAP in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.

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    • Introduction

      Placental alkaline phosphatase (PLAP) is a membrane-associated siaglycoprotein enzyme normally present at high concentration in syncytiotrophoblasts within the placenta during the third trimester of gestation. The expression of PLAP was originally thought to be restricted to term placenta but a human PLAP-like variant has been described which shares more than 85% homology with PLAP itself. PLAP is expressed only in normal term placenta, endocervix and fallopian tube and also in ovarian and proximal gastrointestinal tumors. It is also commonly expressed in germ cell tumors and more recently described in seminomas.

    • Synonyms

      ALP, PLAP, Alkaline phosphatase placental type, EC 3.1.3.1, PLAP-1, Alkaline phosphatase Regan isozyme.

    • Physical Appearance

      Sterile filtered liquid.

    • Stability

      Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Plap Human
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    Description:

    Alkaline Phosphatase Liver/Bone/Kidney Mouse Recombinant

    Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2.

    Product # :

    ENZ-1008

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    Description

    ALPL produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) and having a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    ALPL protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.

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    • Introduction

      Alkaline Phosphatase Liver/Bone/Kidney (Alpl) is a part of the alkaline phosphatases family which comprises 4 related alkaline phosphatases. Alpl is a membrane-bound glycosylated enzyme which is not expressed in any particular tissue. Alpl takes part in skeletal mineralization.

    • Synonyms

      Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS CIGANLDHCA WAGSGLEHHH HHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Alpl Mouse
  • View Data Sheet

    Description:

    Alkaline Phosphatase Human Recombinant

    Alkaline phosphatase liver/bone/kidney isozyme, phosphoamidase, Phosphocreatine phosphatase, aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP.

    Product # :

    ENZ-1190

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    Description

    ALPL Human Recombinant produced in HEK293 is a single, glycosylated polypeptide chain containing 493 amino acids (18-501 a.a) and having a molecular mass of 54.3kDa. ALPL is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

    Source

    HEK293.

    Formulation

    ALPL protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4) & 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 40,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 37C.

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    • Synonyms

      Alkaline phosphatase liver/bone/kidney isozyme, phosphoamidase, Phosphocreatine phosphatase, aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      DGSLVPEKEK DPKYWRDQAQ ETLKYALELQ KLNTNVAKNV IMFLGDGMGV STVTAARILK GQLHHNPGEE TRLEMDKFPF VALSKTYNTN AQVPDSAGTA TAYLCGVKAN EGTVGVSAAT ERSRCNTTQG NEVTSILRWA KDAGKSVGIV TTTRVNHATP SAAYAHSADR DWYSDNEMPP EALSQGCKDI AYQLMHNIRD IDVIMGGGRK YMYPKNKTDV EYESDEKARG TRLDGLDLVD TWKSFKPRYK HSHFIWNRTE LLTLDPHNVD YLLGLFEPGD MQYELNRNNV TDPSLSEMVV VAIQILRKNP KGFFLLVEGG RIDHGHHEGK AKQALHEAVE MDRAIGQAGS LTSSEDTLTV VTADHSHVFT FGGYTPRGNS IFGLAPMLSD TDKKPFTAIL YGNGPGYKVV GGERENVSMV DYAHNNYQAQ SAVPLRHETH GGEDVAVFSK GPMAHLLHGV HEQNYVPHVM AYAACIGANL GHCAPAS HHHHHH.

    • Background

      The ALPL human recombinant, a variant of the alkaline phosphatase enzyme, has emerged as a significant focus of biomedical research due to its diverse biological functions and potential therapeutic applications. Alkaline phosphatase (ALPL) is an essential enzyme involved in various physiological processes, including bone mineralization, liver function, and immune regulation. The ALPL human recombinant, generated through recombinant DNA technology, offers a unique platform to explore the molecular complexity and therapeutic implications of this enzyme.

      Understanding the molecular characteristics of ALPL is crucial to unravel its functional diversity. ALPL belongs to a family of enzymes that hydrolyze phosphate esters under alkaline conditions. The structural features, post-translational modifications, and molecular interactions of ALPL contribute to its complexity and enable its participation in multiple biological processes.

      ALPL plays diverse roles in different tissues and physiological contexts. In bone, ALPL is involved in the regulation of mineralization, ensuring proper skeletal development and maintenance. In the liver, ALPL participates in bile acid metabolism and detoxification processes. Furthermore, ALPL has been implicated in immune regulation and inflammation modulation.

      The therapeutic potential of the ALPL human recombinant is vast, offering opportunities for the diagnosis, treatment, and management of various diseases. ALPL-based therapies hold promise for addressing skeletal disorders, such as hypophosphatasia, where ALPL deficiency leads to impaired bone mineralization. ALPL's involvement in liver function also presents avenues for therapeutic interventions in liver diseases. Moreover, the immunomodulatory properties of ALPL highlight its potential role in immune-related disorders.

      This research aims to provide a comprehensive analysis of the ALPL human recombinant, focusing on its molecular characteristics, biological functions, and therapeutic implications. By exploring the intricate nature of ALPL, we aim to shed light on its therapeutic potential and pave the way for future research in this exciting field.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Alpl Protein
  • View Data Sheet

    Description:

    Alkaline Phosphatase Bovine Intestinal

    EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase.

    Product # :

    ENZ-322

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    Description

    The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

    Source

    Calf Intestine.

    Formulation

    50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.

    Purity

    95% pure by Gel Filtration.

    Biological Activity

    >1500 U/mg (pH 9.6), 25°C and 0.025M glycin, 10% glycerol as buffer.

    More Info

    • Synonyms

      EC 3.1.3.1, IAP, AP, ALPI, Intestinal-type alkaline phosphatase, Intestinal alkaline phosphatase.

    • Physical Appearance

      Sterile Filtered clear colorless solution.

    • Stability

      AP should be stored at 4°C. Please Do-Not freeze.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Alkaline Phosphatase

About Alkaline Phosphatase:

Alkaline phosphatase also known as basic phosphatase is anenzyme with homodimer structure. Every monomer unit holds 5 cysteine residues, 2 zinc atoms & a magnesium atom which are critical to the catalytic function of the enzyme, also, it is important for the enzyme’s activity in alkaline pH. Alkaline phosphatase dephosphorylates compounds as its main role. The enzyme is active in a large quantity of prokaryotes and eukaryotes organisms, with a constant function but in various structural forms suited to the environment they are active in.