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3 results found for “CD73”

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  • View Data Sheet

    Description:

    5'-Nucleotidase Ecto (CD73) Human Recombinant

    NT, eN, NT5, NTE, eNT, CD73, E5NT, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E.

    Product # :

    PRO-1853

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    Description

    NT5E (CD73) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 247amino acids (27-252 a.a) and having a molecular mass of 27kDa. NT5E (CD73) is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    E.coli.

    Formulation

    NT5E (CD73) protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

    Purity

    Greater than 90% as determined by SDS-PAGE.

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    • Introduction

      NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.

    • Synonyms

      NT, eN, NT5, NTE, eNT, CD73, E5NT, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, NT5E.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MWELTILHTN DVHSRLEQTS EDSSKCVNAS RCMGGVARLF TKVQQIRRAE PNVLLLDAGD QYQGTIWFTV YKGAEVAHFM NALRYDAMAL GNHEFDNGVE GLIEPLLKEA KFPILSANIK AKGPLASQIS GLYLPYKVLP VGDEVVGIVG YTSKETPFLS NPGTNLVFED EITALQPEVD KLKTLNVNKI IALGHSGFEM DKLIAQKVRG VDVVVGGHSN TFLYTGN.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nt5E Cd73 Human
  • View Data Sheet

    Description:

    5'-Nucleotidase Ecto (CD73) Mouse Recombinant

    5'-nucleotidase (EC:3.1.3.5), 5'-NT, Ecto-5'-nucleotidase, CD73, Nt5e, Nt5, Nte.

    Product # :

    PRO-2386

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    Description

    NT5E Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 531 amino acids (29-551a.a.) and having a molecular mass of 59.1kDa. NT5E is expressed with a 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    NT5E protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 90% as determined by SDS-PAGE.

    Biological Activity

    Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25°C.

    More Info

    • Introduction

      NT5E, also known as CD73, is a plasma membrane protein which catalyzes the conversion of extracellular nucleotides into membrane-permeable nucleosides. NT5E is functions as a determinant of lymphocyte differentiation. Defects in NT5E can cause the calcification of joints and arteries.

    • Synonyms

      5'-nucleotidase (EC:3.1.3.5), 5'-NT, Ecto-5'-nucleotidase, CD73, Nt5e, Nt5, Nte.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFSLEHHHHH H.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Nt5E Cd73 Mouse
  • View Data Sheet

    Description:

    CD73, Mouse Anti Human

    5'-nucleotidase, Ecto-5'-nucleotidase, 5'-NT, CD73, NT5E, NT5, NTE, NT, eN, eNT, E5NT.

    Product # :

    ANT-414

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    Formulation

    1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

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    • Introduction

      Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP at a neutral pH. CD73, which consists of a dimer of 2 identical 70kDa subunits, is bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Additional forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.

    • Synonyms

      5'-nucleotidase, Ecto-5'-nucleotidase, 5'-NT, CD73, NT5E, NT5, NTE, NT, eN, eNT, E5NT.

    • Physical Appearance

      Sterile filtered colorless solution.

    • Immunogen

      Anti-human CD73 mAb , is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CD73 amino acids 27-252 purified from E. coli.

    • Ig Subclass

      Mouse IgG2b heavy chain and κ light chain.

    • Clone

      P2B6AT.

    • Applications

      CD73 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

    • Type

      Mouse Anti Human Monoclonal.

    • Storage Procedures

      For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

    • Purification Method

      CD73 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Cd73 Antibody