HRP

HRP

Enzymes

Name

Description

Product #

Price

Quantity

Shipping Method

  • Name :

    HRP

    Description:

    Horseradish Peroxidase

    Horseradish Peroxidase, HRP, EC 1.11.1.7.

    Product # :

    ENZ-321

    Price :

    Quantity :

    Shipping Method :

    Room Temp Icon

    Shipped at Room temp

    Add :

  • description
  • source
  • purity
  • biological activity
  • More Info

Description

HRP consists of the basic isoenzyme having a molecular weight of 44 kDa.The Horseradish Peroxidase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

Source

Root extracts of horseradish.

Purity

(A403/A275) = RZ: 3.0.

Biological Activity

276 U/mg (25°C, guaiacol as the hydrogen donor, pH-7 and H2O2 as substrates).

More Info

  • Introduction

    The enzyme horseradish peroxidase, found in horseradish, is used extensively in molecular biologyand in antibody amplification and detection, among other things. For example, "In recent years the technique of marking neurons with the enzyme horseradish peroxidase (HRP) has become a major tool. In its brief history, this method has probably been used by more neurobiologists than have used the Golgi stainsince its discovery in 1870." Horseradish peroxidase is also highly used in techniques such as Western blottingand ELISAs.
    HRP is widely used as an enzymatic label in immunoassays. Usually, the enzyme is coupled to antibodies, lectins or haptens. Coupling to antibodies etc. may be performed through the carbohydrate side chains of the HRP.

  • Synonyms

    Horseradish Peroxidase, HRP, EC 1.11.1.7.

  • Physical Appearance

    Sterile Filtered red-brown lyophilized powder.

  • Stability

    Lyophilized HRP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HRP should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized HRP in sterile 18MΩ-cm H2O not less than 100 µg/ml.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Horseradish Peroxidase

You may also like