DNase Human

DNase Human

Enzymes

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  • Name :

    DNase Human

    Description:

    Deoxyribonuclease I Human Recombinant

    EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1.

    Product # :

    ENZ-319

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    Quantity :

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Description

Deoxyribonuclease I Human Recombinant produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1999N339O396S9. DNase is purified by proprietary chromatographic techniques.

Source

Chinese Hamster Ovary Cells.

Formulation

Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.

More Info

  • Introduction

    Deoxyribonuclease I Human Recombinant (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2 .
    Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.

  • Synonyms

    EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1.

  • Physical Appearance

    Sterile liquid colorless solution at a concentration of 1mg/ml.

  • Stability

    2 years when stored at 4°C, three weeks at 15°C, pH-6.3.

  • Unit Definition

    Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1µgof lambda phage DNA in 10 minutes at 37°C in50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.

  • Specific Activity

    1000IU/1mg.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Dnase I Human

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