APOB Protein

APOB, APO-B, Apolipoprotein B.

The APOBHuman is created as a recombinant protein with a 4kda N-terminal fusion of His Tag. The APOBHis-Tagged Fusion Protein, produced in E. coli, is a 31kDa protein containing 201 amino acid residues of the APOBHuman, 1406-1606 amino acids.

Escherichia Coli.

Filtered White lyophilized (freeze-dried) powder.

Each mg was lyophilized with 1xPBS, 0.4% SDS and 4mM DTT.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Store lyophilized APOBat -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Greater than 80% as determined by SDS-PAGE.

SDS

Apolipoprotein-B (ApoB) is the main apolipoprotein of LDL, VLDL, IDL and chylomicrons particles which serves as the carrier of lipids in the water surrounding the cells in every tissue across the body. Apolipoprotein B acts as the key organizing protein of all other carriers of lipids. across LDL membranes, ApoB also plays a role as a ligand for LDL receptors in many cells across the body, meaning, it shows that lipid carriers that are set to cross into cells with Apolipoprotein B receptors, this is how lipids are transported within and into cells.